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Yorodumi- PDB-1t1h: NMR solution structure of the U box domain from AtPUB14, an armad... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1t1h | ||||||
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Title | NMR solution structure of the U box domain from AtPUB14, an armadillo repeat containing protein from Arabidopsis thaliana | ||||||
Components | armadillo repeat containing protein | ||||||
Keywords | LIGASE / UBIQUITIN LIGASE / E3 LIGASE / ARABIDOPSIS / U-BOX | ||||||
Function / homology | Function and homology information transmembrane receptor protein serine/threonine kinase binding / RING-type E3 ubiquitin transferase / ubiquitin-protein transferase activity / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Arabidopsis thaliana (thale cress) | ||||||
Method | SOLUTION NMR / torsion angle dynamics, simulated annealing | ||||||
Authors | Andersen, P. / Kragelund, B.B. / Olsen, A.N. / Larsen, F.H. / Chua, N.-H. / Poulsen, F.M. / Skriver, K. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2004 Title: Structure and Biochemical Function of a Prototypical Arabidopsis U-box Domain Authors: Andersen, P. / Kragelund, B.B. / Olsen, A.N. / Larsen, F.H. / Chua, N.-H. / Poulsen, F.M. / Skriver, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1t1h.cif.gz | 472.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1t1h.ent.gz | 411.2 KB | Display | PDB format |
PDBx/mmJSON format | 1t1h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/t1/1t1h ftp://data.pdbj.org/pub/pdb/validation_reports/t1/1t1h | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8762.994 Da / Num. of mol.: 1 / Fragment: U-box domain (249-321) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Plasmid: pGEX-4T-1 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q8VZ40 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy |
-Sample preparation
Details |
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Sample conditions | Ionic strength: 20 mM sodium phosphate, 0.15M NaCl / pH: 7.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics, simulated annealing / Software ordinal: 1 Details: the structures are based on a total of 1083 NOE-derived distance constraints and 88 dihedral angle contraints | ||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with favorable non-bond energy, structures with the least restraint violations Conformers calculated total number: 200 / Conformers submitted total number: 20 |