+Open data
-Basic information
Entry | Database: PDB / ID: 1rv0 | |||||||||
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Title | 1930 Swine H1 Hemagglutinin complexed with LSTA | |||||||||
Components | (hemagglutinin) x 2 | |||||||||
Keywords | VIRAL PROTEIN / Hemagglutinin / Influenza A Virus | |||||||||
Function / homology | Function and homology information viral budding from plasma membrane / clathrin-dependent endocytosis of virus by host cell / host cell surface receptor binding / apical plasma membrane / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane Similarity search - Function | |||||||||
Biological species | Influenza A virus | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | |||||||||
Authors | Skehel, J.J. / Gamblin, S.J. / Haire, L.F. / Russell, R.J. / Stevens, D.J. / Xiao, B. / Ha, Y. / Vasisht, N. / Steinhauer, D.A. / Daniels, R.S. | |||||||||
Citation | Journal: Science / Year: 2004 Title: The structure and receptor binding properties of the 1918 influenza hemagglutinin. Authors: Gamblin, S.J. / Haire, L.F. / Russell, R.J. / Stevens, D.J. / Xiao, B. / Ha, Y. / Vasisht, N. / Steinhauer, D.A. / Daniels, R.S. / Elliot, A. / Wiley, D.C. / Skehel, J.J. | |||||||||
History |
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Remark 999 | Author states that the sequence of this protein is correct and there is no matched sequence ...Author states that the sequence of this protein is correct and there is no matched sequence database available. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1rv0.cif.gz | 301.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1rv0.ent.gz | 244.5 KB | Display | PDB format |
PDBx/mmJSON format | 1rv0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rv/1rv0 ftp://data.pdbj.org/pub/pdb/validation_reports/rv/1rv0 | HTTPS FTP |
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-Related structure data
Related structure data | 1ru7C 1ruySC 1ruzC 1rvtC 1rvxC 1rvzC C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 36076.352 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Details: Bromelain digested / Source: (natural) Influenza A virus / Genus: Influenzavirus A / Strain: A-SWINE-IOWA-30 / References: UniProt: Q82500 #2: Protein | Mass: 18180.053 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Details: Bromelain digested / Source: (natural) Influenza A virus / Genus: Influenzavirus A / Strain: A-SWINE-IOWA-30 / References: UniProt: Q82500 #3: Sugar | #4: Sugar | ChemComp-DAN / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.38 Å3/Da / Density % sol: 71.9 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6.8 Details: PEG3350, TrisHCl, Sodium citrate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX9.6 / Wavelength: 0.9794 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Aug 6, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9794 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→20 Å / Num. all: 97658 / Num. obs: 85973 / % possible obs: 88.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 10.1 % / Rmerge(I) obs: 0.076 / Net I/σ(I): 15.9 |
Reflection shell | Resolution: 2.5→2.59 Å / Rmerge(I) obs: 0.438 / Mean I/σ(I) obs: 2 / % possible all: 78.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 1RUY Resolution: 2.5→20 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.5→20 Å
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