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Yorodumi- PDB-1r1t: Crystal structure of the cyanobacterial metallothionein repressor... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1r1t | ||||||
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Title | Crystal structure of the cyanobacterial metallothionein repressor SmtB in the apo-form | ||||||
Components | Transcriptional repressor smtB | ||||||
Keywords | TRANSCRIPTION REPRESSOR / Zinc / transcriptional regulation / winged HTH protein / DNA binding | ||||||
Function / homology | Function and homology information DNA-binding transcription factor activity / DNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | Synechococcus elongatus PCC 7942 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.7 Å | ||||||
Authors | Eicken, C. / Pennella, M.A. / Chen, X. / Koshlap, K.M. / VanZile, M.L. / Sacchettini, J.C. / Giedroc, D.P. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2003 Title: A metal-ligand-mediated intersubunit allosteric switch in related SmtB/ArsR zinc sensor proteins. Authors: Eicken, C. / Pennella, M.A. / Chen, X. / Koshlap, K.M. / VanZile, M.L. / Sacchettini, J.C. / Giedroc, D.P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1r1t.cif.gz | 57.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1r1t.ent.gz | 41.1 KB | Display | PDB format |
PDBx/mmJSON format | 1r1t.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/r1/1r1t ftp://data.pdbj.org/pub/pdb/validation_reports/r1/1r1t | HTTPS FTP |
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-Related structure data
Related structure data | 1r1uC 1r1vC 1r22C 1r23C 1smtS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | The asymmetric unit contains the dimeric biological assembly. |
-Components
#1: Protein | Mass: 13561.474 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Synechococcus elongatus PCC 7942 (bacteria) Species: Synechococcus elongatus / Strain: PCC7942 / Gene: SMTB / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P30340 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.06 Å3/Da / Density % sol: 40.22 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7 Details: Na/K phosphate, Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 14-BM-C / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Mar 7, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→60 Å / Num. all: 24148 / Num. obs: 22325 / % possible obs: 92.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 4.3 % / Rsym value: 0.029 / Net I/σ(I): 19.9 |
Reflection shell | Resolution: 1.7→1.76 Å / Rsym value: 0.185 / % possible all: 58.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1SMT Resolution: 1.7→60 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.7→60 Å
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Refine LS restraints |
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Xplor file |
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