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Yorodumi- PDB-1ps2: HIGH RESOLUTION NMR SOLUTION STRUCTURE OF HUMAN PS2, 19 STRUCTURES -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ps2 | ||||||
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Title | HIGH RESOLUTION NMR SOLUTION STRUCTURE OF HUMAN PS2, 19 STRUCTURES | ||||||
Components | PS2 | ||||||
Keywords | GROWTH FACTOR / CELL MOTILITY / TUMOR SUPPRESSOR / TREFOIL DOMAIN | ||||||
Function / homology | Function and homology information maintenance of gastrointestinal epithelium / response to iron ion / response to immobilization stress / growth factor activity / response to peptide hormone / cell population proliferation / Estrogen-dependent gene expression / cell differentiation / carbohydrate metabolic process / negative regulation of cell population proliferation ...maintenance of gastrointestinal epithelium / response to iron ion / response to immobilization stress / growth factor activity / response to peptide hormone / cell population proliferation / Estrogen-dependent gene expression / cell differentiation / carbohydrate metabolic process / negative regulation of cell population proliferation / extracellular space / extracellular region Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Williams, M.A. / Polshakov, V.I. / Gargaro, A.R. / Feeney, J. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1997 Title: High-resolution solution structure of human pNR-2/pS2: a single trefoil motif protein. Authors: Polshakov, V.I. / Williams, M.A. / Gargaro, A.R. / Frenkiel, T.A. / Westley, B.R. / Chadwick, M.P. / May, F.E. / Feeney, J. #1: Journal: Biochem.J. / Year: 1995 Title: Production and Comparison of Mature Single-Domain 'Trefoil' Peptides Pnr-2/Ps2 Cys58 and Pnr-2/Ps2 Ser58 Authors: Chadwick, M.P. / May, F.E. / Westley, B.R. #2: Journal: Eur.J.Biochem. / Year: 1995 Title: NMR-Based Structural Studies of the Pnr-2/Ps2 Single Domain Trefoil Peptide. Similarities to Porcine Spasmolytic Peptide and Evidence for a Monomeric Structure Authors: Polshakov, V.I. / Frenkiel, T.A. / Westley, B. / Chadwick, M. / May, F. / Carr, M.D. / Feeney, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ps2.cif.gz | 324.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ps2.ent.gz | 280.9 KB | Display | PDB format |
PDBx/mmJSON format | 1ps2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ps/1ps2 ftp://data.pdbj.org/pub/pdb/validation_reports/ps/1ps2 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 6662.300 Da / Num. of mol.: 1 / Mutation: C58S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Tissue: EPITHELIAL / Cell: MCF-7, UACL / Cell line: HB101 / Cellular location: EXTRACELLULARGlossary of biology / Gene: BCEI / Organ: BREAST, STOMACH / Cell line (production host): HB101 / Gene (production host): BCEI / Production host: Escherichia coli (E. coli) / Strain (production host): JM109 / References: UniProt: P04155 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Sample conditions | pH: 6 / Temperature: 298 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
Software |
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NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR ensemble | Conformer selection criteria: TOTAL NOE CONSTRAINT VIOLATIONS < 1.0 A Conformers calculated total number: 20 / Conformers submitted total number: 19 |