+Open data
-Basic information
Entry | Database: PDB / ID: 1pc2 | ||||||
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Title | Solution structure of human mitochondria fission protein Fis1 | ||||||
Components | mitochondria fission protein | ||||||
Keywords | UNKNOWN FUNCTION / Mitochondria / Fission | ||||||
Function / homology | Function and homology information negative regulation of fatty acid transport / negative regulation of ATP metabolic process / Class I peroxisomal membrane protein import / positive regulation of mitochondrial calcium ion concentration / peroxisome fission / mitochondrial fragmentation involved in apoptotic process / negative regulation of endoplasmic reticulum calcium ion concentration / protein targeting to mitochondrion / mitochondrial fission / peroxisomal membrane ...negative regulation of fatty acid transport / negative regulation of ATP metabolic process / Class I peroxisomal membrane protein import / positive regulation of mitochondrial calcium ion concentration / peroxisome fission / mitochondrial fragmentation involved in apoptotic process / negative regulation of endoplasmic reticulum calcium ion concentration / protein targeting to mitochondrion / mitochondrial fission / peroxisomal membrane / regulation of mitochondrion organization / : / mitochondrial fusion / autophagy of mitochondrion / positive regulation of mitochondrial fission / positive regulation of cysteine-type endopeptidase activity involved in apoptotic process / calcium-mediated signaling using intracellular calcium source / positive regulation of protein targeting to membrane / positive regulation of intrinsic apoptotic signaling pathway / release of cytochrome c from mitochondria / peroxisome / positive regulation of cytosolic calcium ion concentration / mitochondrial outer membrane / molecular adaptor activity / lipid binding / endoplasmic reticulum / protein-containing complex / mitochondrion / membrane / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / Distance geometry, Simulated annealing, Molecular dynamics | ||||||
Authors | Suzuki, M. / Youle, R.J. / Tjandra, N. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2003 Title: The Solution Structure of Human Mitochondria Fission Protein Fis1 Reveals a Novel TPR-like Helix Bundle Authors: Suzuki, M. / Jeong, S.Y. / Karbowski, M. / Youle, R.J. / Tjandra, N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1pc2.cif.gz | 939.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1pc2.ent.gz | 816.2 KB | Display | PDB format |
PDBx/mmJSON format | 1pc2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pc/1pc2 ftp://data.pdbj.org/pub/pdb/validation_reports/pc/1pc2 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 17229.887 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: Fis1 / Plasmid: pET21 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q9Y3D6 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | Ionic strength: 10mM Tris Acetate / pH: 5.5 / Pressure: AMBIENT / Temperature: 305 K | ||||||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: Distance geometry, Simulated annealing, Molecular dynamics Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |