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- PDB-1nnv: The Solution structure of HI1450 -

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Basic information

Entry
Database: PDB / ID: 1nnv
TitleThe Solution structure of HI1450
ComponentsHypothetical protein HI1450Hypothesis
KeywordsUNKNOWN FUNCTION / HYPOTHETICAL PROTEIN / Structure 2 Function Project / S2F / Structural Genomics
Function / homologydsDNA mimic, putative / dsDNA mimic, putative / Putative dsDNA mimic superfamily / Protein of unknown function, DUF440 / Nuclear Transport Factor 2; Chain: A, / Roll / Alpha Beta / UPF0263 protein HI_1450
Function and homology information
Biological speciesHaemophilus influenzae (bacteria)
MethodSOLUTION NMR
AuthorsParsons, L.M. / Orban, J. / Structure 2 Function Project (S2F)
CitationJournal: Proteins / Year: 2004
Title: Solution structure of the highly acidic protein HI1450 from Haemophilus influenzae, a putative double-stranded DNA mimic.
Authors: Parsons, L.M. / Yeh, D.C. / Orban, J.
History
DepositionJan 14, 2003Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 27, 2004Provider: repository / Type: Initial release
Revision 1.1Apr 29, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 23, 2022Group: Data collection / Database references / Derived calculations
Category: database_2 / pdbx_nmr_software ...database_2 / pdbx_nmr_software / pdbx_struct_assembly / pdbx_struct_oper_list / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_nmr_software.name / _struct_ref_seq_dif.details

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Hypothetical protein HI1450


Theoretical massNumber of molelcules
Total (without water)12,8171
Polymers12,8171
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 65structures with the least restraint violations
RepresentativeModel #4closest to the average

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Components

#1: Protein Hypothetical protein HI1450 / Hypothesis


Mass: 12817.129 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Haemophilus influenzae (bacteria) / Gene: HI1450 / Plasmid: pET15b / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 DE3 / References: UniProt: P44199

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1113D 13C-separated NOESY
1223D 15N-separated NOESY
1312D NOESY
141HNHA

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Sample preparation

Details
Solution-IDContentsSolvent system
150mM phosphate, 100mM NaCl,90% H20, 10% D2090% H2O/10% D2O
250mM phosphate, 100mM NaCl99% D2O
Sample conditionsIonic strength: 50mM phosphate, 100mM NaCl / pH: 6.7 / Pressure: ambient / Temperature: 298 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelengthRelative weight: 1
NMR spectrometerType: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz

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Processing

NMR software
NameVersionDeveloperClassification
CNS1.1Brunger et al.structure solution
NMRPipe5.0.3Bruce A. Johnsonprocessing
Sparky3.106T. D. Goddard and D. G. Knellerdata analysis
NOEID1L. Parsons & J. Orbandata analysis
CNS1.1Brunger et al.refinement
NMR representativeSelection criteria: closest to the average
NMR ensembleConformer selection criteria: structures with the least restraint violations
Conformers calculated total number: 65 / Conformers submitted total number: 20

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