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Yorodumi- PDB-1mm5: Solution NMR structure of the outer membrane enzyme PagP in OG mi... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1mm5 | ||||||
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Title | Solution NMR structure of the outer membrane enzyme PagP in OG micelles | ||||||
Components | CrcA protein | ||||||
Keywords | TRANSFERASE / eta barrel / Palmitoyltransferase | ||||||
Function / homology | Function and homology information lipid IVA palmitoyltransferase / O-palmitoyltransferase activity / lipid A biosynthetic process / cell outer membrane Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Hwang, P.M. / Choy, W.-Y. / Lo, E.I. / Chen, L. / Forman-Kay, J.D. / Raetz, C.R.H. / Prive, G.G. / Bishop, R.E. / Kay, L.E. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2002 Title: Solution Structure and Dynamics of the Outer Membrane Enzyme PagP by NMR Authors: Hwang, P.M. / Choy, W.-Y. / Lo, E.I. / Chen, L. / Forman-Kay, J.D. / Raetz, C.R.H. / Prive, G.G. / Bishop, R.E. / Kay, L.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1mm5.cif.gz | 1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1mm5.ent.gz | 931.6 KB | Display | PDB format |
PDBx/mmJSON format | 1mm5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mm/1mm5 ftp://data.pdbj.org/pub/pdb/validation_reports/mm/1mm5 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 20198.434 Da / Num. of mol.: 1 / Mutation: signal sequence replaced by Met Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: CrcA / Plasmid: pET21a / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P37001 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 4D 15N-separated NOESY |
-Sample preparation
Details | Contents: 1 mM PagP U-2H,15N,13C; 50 mM phosphate buffer; 200 mM OG Solvent system: 90% H2O, 10% D2O, 1% ethanol |
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Sample conditions | Ionic strength: 50 mM phosphate / pH: 6.0 / Pressure: 1 atm / Temperature: 318 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 250 / Conformers submitted total number: 20 |