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- PDB-1lqc: LAC REPRESSOR HEADPIECE (RESIDUES 1-56), NMR, 32 STRUCTURES -

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Entry
Database: PDB / ID: 1lqc
TitleLAC REPRESSOR HEADPIECE (RESIDUES 1-56), NMR, 32 STRUCTURES
ComponentsLAC REPRESSOR
KeywordsTRANSCRIPTION REGULATION / LAC OPERON / LAC REPRESSOR / HEADPIECE / DNA
Function / homology
Function and homology information


DNA-binding transcription repressor activity / cis-regulatory region sequence-specific DNA binding / transcription cis-regulatory region binding / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / regulation of DNA-templated transcription / identical protein binding / cytosol
Similarity search - Function
Transcriptional regulator LacI/GalR-like, sensor domain / LacI-type HTH domain signature. / Periplasmic binding protein-like domain / LacI-type HTH domain / Bacterial regulatory proteins, lacI family / LacI-type HTH domain profile. / helix_turn _helix lactose operon repressor / lambda repressor-like DNA-binding domains / 434 Repressor (Amino-terminal Domain) / Lambda repressor-like, DNA-binding domain superfamily ...Transcriptional regulator LacI/GalR-like, sensor domain / LacI-type HTH domain signature. / Periplasmic binding protein-like domain / LacI-type HTH domain / Bacterial regulatory proteins, lacI family / LacI-type HTH domain profile. / helix_turn _helix lactose operon repressor / lambda repressor-like DNA-binding domains / 434 Repressor (Amino-terminal Domain) / Lambda repressor-like, DNA-binding domain superfamily / Periplasmic binding protein-like I / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Lactose operon repressor
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodSOLUTION NMR
AuthorsSlijper, M. / Bonvin, A.M.J.J. / Boelens, R. / Kaptein, R.
Citation
Journal: J.Mol.Biol. / Year: 1996
Title: Refined structure of lac repressor headpiece (1-56) determined by relaxation matrix calculations from 2D and 3D NOE data: change of tertiary structure upon binding to the lac operator.
Authors: Slijper, M. / Bonvin, A.M. / Boelens, R. / Kaptein, R.
#1: Journal: To be Published
Title: Backbone and Sidechain Dynamics of Lac Repressor Headpiece (1-56) and its Complex with DNA
Authors: Slijper, M. / Boelens, R. / Davis, A.L. / Konings, R.N.H. / Van Der Marel, G.A. / Van Bloom, J.H.
#2: Journal: J.Magn.Reson.,Ser.B / Year: 1995
Title: Application of Structure Refinement Using 3D Noe-Noe Spectroscopy to Lac Repressor Headpiece (1-56)
Authors: Slijper, M. / Bonvin, M.J.J. / Boelens, R. / Kaptein, R.
#3: Journal: J.Mol.Biol. / Year: 1993
Title: Structure of the Complex of Lac Repressor Headpiece and an 11 Base-Pair Half-Operator Determined by Nuclear Magnetic Resonance Spectroscopy and Restrained Molecular Dynamics
Authors: Chuprina, V.P. / Rullmann, J.A. / Lamerichs, R.M. / Van Boom, J.H. / Boelens, R. / Kaptein, R.
#4: Journal: Biopolymers / Year: 1985
Title: Two-Dimensional 1H-NMR Studies on the Lac Repressor DNA Binding Domain: Further Resonance Assignments and Identification of Nuclear Overhauser Enhancements
Authors: Zuiderweg, E.R. / Scheek, R.M. / Kaptein, R.
#5: Journal: Biopolymers / Year: 1985
Title: Stereospecific Assignments of 1H-NMR Methyl Lines and Conformation of Valyl Residues in the Lac Repressor Headpiece
Authors: Zuiderweg, E.R.P. / Boelens, R. / Kaptein, R.
#6: Journal: Eur.J.Biochem. / Year: 1983
Title: Sequence-Specific Resonance Assignments in the 1H Nuclear-Magnetic-Resonance Spectrum of the Lac Repressor DNA-Binding Domain 1-51 from Escherichia Coli by Two-Dimensional Spectroscopy
Authors: Zuiderweg, E.R. / Kaptein, R. / Wuthrich, K.
History
DepositionAug 13, 1996Processing site: BNL
Revision 1.0Feb 12, 1997Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 23, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site

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Assembly

Deposited unit
A: LAC REPRESSOR


Theoretical massNumber of molelcules
Total (without water)6,2331
Polymers6,2331
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)32 / -
Representative

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Components

#1: Protein LAC REPRESSOR / / LAC HP56


Mass: 6233.070 Da / Num. of mol.: 1 / Fragment: HEADPIECE, RESIDUES 1 - 56
Source method: isolated from a genetically manipulated source
Details: THE PROTEIN CONTAINS THE FIRST 56 N-TERMINAL RESIDUES (I.E., THE DNA BINDING REGION) OF THE COMPLETE LAC REPRESSOR PROTEIN
Source: (gene. exp.) Escherichia coli (E. coli) / Description: T7 RNA POLYMERASE PROMOTER SYSTEM / Gene: LAC I THE PART ENCODING ONLY / Variant: DH9 / Plasmid: PET-HP56
Gene (production host): LAC I, THE PART ENCODING ONLY THE FIRST 56 AMINO ACIDS
Production host: Escherichia coli (E. coli) / References: UniProt: P03023

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Crystal grow
*PLUS
Method: other / Details: NMR

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Processing

NMR softwareName: DINOSAUR / Developer: BONVIN,VIS,BREG,BURGERING,BOELENS,KAPTEIN / Classification: refinement
NMR ensembleConformers submitted total number: 32

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