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Yorodumi- PDB-1lcd: STRUCTURE OF THE COMPLEX OF LAC REPRESSOR HEADPIECE AND AN 11 BAS... -
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-Basic information
Entry | Database: PDB / ID: 1lcd | ||||||
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Title | STRUCTURE OF THE COMPLEX OF LAC REPRESSOR HEADPIECE AND AN 11 BASE-PAIR HALF-OPERATOR DETERMINED BY NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY AND RESTRAINED MOLECULAR DYNAMICS | ||||||
Components |
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Keywords | GENE REGULATION/DNA / GENE REGULATION-DNA complex | ||||||
Function / homology | Function and homology information DNA-binding transcription repressor activity / cis-regulatory region sequence-specific DNA binding / transcription cis-regulatory region binding / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / regulation of DNA-templated transcription / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR | ||||||
Authors | Chuprina, V.P. / Rullmann, J.A.C. / Lamerichs, R.M.J.N. / Van Boom, J.H. / Boelens, R. / Kaptein, R. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1993 Title: Structure of the complex of lac repressor headpiece and an 11 base-pair half-operator determined by nuclear magnetic resonance spectroscopy and restrained molecular dynamics. Authors: Chuprina, V.P. / Rullmann, J.A. / Lamerichs, R.M. / van Boom, J.H. / Boelens, R. / Kaptein, R. #1: Journal: Eur.J.Biochem. / Year: 1990 Title: Assignment of the 1H-NMR Spectrum of a Lac Repressor Headpiece-Operator Complex in H2O and Identification of Noes. Consequences for Protein-DNA Interaction Authors: Lamerichs, R.M.J.N. / Boelens, R. / Van Der Marel, G.A. / Van Boom, J.H. / Kaptein, R. #2: Journal: Biochem.Pharm. / Year: 1990 Title: Two-Dimensional NMR Study of a Protein-DNA Complex. Lac Repressor Headpiece-Operator Interaction Authors: Kaptein, R. / Lamerichs, R.M.J.N. / Boelens, R. / Rullmann, J.A.C. #3: Journal: Biochemistry / Year: 1989 Title: 1H NMR Study of a Complex between the Lac Repressor Headpiece and a 22 Base Pair Symmetric Lac Operator Authors: Lamerichs, R.M.J.N. / Boelens, R. / Van Der Marel, G.A. / Van Boom, J.H. / Kaptein, R. / Buck, F. / Fera, B. / Rueterjans, H. #4: Journal: Ucla Symp.Mol.Cell.Biol., New Ser. / Year: 1989 Title: NMR Based Docking Studies of Lac Repressor Headpiece on a Lac Operator Fragment Authors: Rullmann, J.A.C. / Boelens, R. / Kaptein, R. #5: Journal: Protein Seq.Data Anal. / Year: 1988 Title: The Interaction of Lac Repressor Headpiece with its Operator: An NMR View Authors: Boelens, R. / Lamerichs, R.M.J.N. / Rullmann, J.A.C. / Van Boom, J.H. / Kaptein, R. #6: Journal: Proteins / Year: 1988 Title: Combined Procedure of Distance Geometry and Restrained Molecular Dynamics Techniques for Protein Structure Determination from Nuclear Magnetic Resonance Data: Application to the DNA Binding ...Title: Combined Procedure of Distance Geometry and Restrained Molecular Dynamics Techniques for Protein Structure Determination from Nuclear Magnetic Resonance Data: Application to the DNA Binding Domain of Lac Repressor from Escherichia Coli Authors: De Vlieg, J. / Scheek, R.M. / Van Gunsteren, W.F. / Berendsen, H.J.C. / Kaptein, R. / Thomason, J. #7: Journal: Nato Asi Ser.,Ser.A / Year: 1987 Title: A Two-Dimensional NMR Study of the Complex of Lac Repressor Headpiece with a 14 Base Pair Lac Operator Fragment Authors: Boelens, R. / Scheek, R.M. / Lamerichs, R.M.J.N. / De Vlieg, J. / Van Boom, J.H. / Kaptein, R. #8: Journal: J.Mol.Biol. / Year: 1987 Title: Complex of Lac Repressor Headpiece with a 14 Base Pair Lac Operator Fragment Studied by Two-Dimensional Nuclear Magnetic Resonance Authors: Boelens, R. / Scheek, R.M. / Van Boom, J.H. / Kaptein, R. #9: Journal: Isr.J.Chem. / Year: 1986 Title: Restrained Molecular Dynamics Procedure for Protein Tertiary Structure Determination from NMR Data: A Lac Repressor Headpiece Structure Based on Information on J-Coupling and from Presence and Absence of Noes Authors: De Vlieg, J. / Boelens, R. / Scheek, R.M. / Kaptein, R. / Van Gunsteren, W.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1lcd.cif.gz | 78.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1lcd.ent.gz | 56.5 KB | Display | PDB format |
PDBx/mmJSON format | 1lcd.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lc/1lcd ftp://data.pdbj.org/pub/pdb/validation_reports/lc/1lcd | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: DNA chain | Mass: 3413.246 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: DNA chain | Mass: 3293.174 Da / Num. of mol.: 1 / Source method: obtained synthetically |
#3: Protein | Mass: 5693.446 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / References: UniProt: P03023 |
#4: Chemical | ChemComp-NA / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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-Sample preparation
Crystal grow | *PLUS Method: other / Details: NMR |
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-Processing
NMR ensemble | Conformers submitted total number: 3 |
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