+Open data
-Basic information
Entry | Database: PDB / ID: 1lbh | ||||||
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Title | INTACT LACTOSE OPERON REPRESSOR WITH GRATUITOUS INDUCER IPTG | ||||||
Components | INTACT LACTOSE OPERON REPRESSOR WITH GRATUITOUS INDUCER IPTG | ||||||
Keywords | TRANSCRIPTION REGULATION / DNA-BINDING / REPRESSOR | ||||||
Function / homology | Function and homology information DNA-binding transcription repressor activity / cis-regulatory region sequence-specific DNA binding / transcription cis-regulatory region binding / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / regulation of DNA-templated transcription / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 3.2 Å | ||||||
Authors | Lewis, M. / Chang, G. / Horton, N.C. / Kercher, M.A. / Pace, H.C. / Lu, P. | ||||||
Citation | Journal: Science / Year: 1996 Title: Crystal structure of the lactose operon repressor and its complexes with DNA and inducer. Authors: Lewis, M. / Chang, G. / Horton, N.C. / Kercher, M.A. / Pace, H.C. / Schumacher, M.A. / Brennan, R.G. / Lu, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1lbh.cif.gz | 222.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1lbh.ent.gz | 178.5 KB | Display | PDB format |
PDBx/mmJSON format | 1lbh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lb/1lbh ftp://data.pdbj.org/pub/pdb/validation_reports/lb/1lbh | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 38657.051 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: GM1 / Gene: LAC I / Plasmid: PIQ / Gene (production host): LAC I / Production host: Escherichia coli (E. coli) / References: UniProt: P03023 #2: Sugar | ChemComp-IPT / |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.56 Å3/Da / Density % sol: 51.9 % | |||||||||||||||||||||||||||||||||||||||||||||
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Crystal | *PLUS Density % sol: 65.5 % | |||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 22 ℃ / pH: 8.5 / Method: macro seeding | |||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: Nov 18, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 26094 / % possible obs: 97 % / Rmerge(I) obs: 0.091 |
Reflection | *PLUS Highest resolution: 3.2 Å / Lowest resolution: 15 Å / Num. measured all: 110526 |
-Processing
Software |
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Refinement | Resolution: 3.2→15 Å / Rfactor Rwork: 0.23 / Rfactor obs: 0.23 / σ(F): 0 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 22 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.2→15 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.23 / Rfactor Rwork: 0.23 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |