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Yorodumi- PDB-1iam: STRUCTURE OF THE TWO AMINO-TERMINAL DOMAINS OF HUMAN INTERCELLULA... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1iam | ||||||
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Title | STRUCTURE OF THE TWO AMINO-TERMINAL DOMAINS OF HUMAN INTERCELLULAR ADHESION MOLECULE-1, ICAM-1 | ||||||
Components | INTERCELLULAR ADHESION MOLECULE-1 | ||||||
Keywords | Viral protein receptor / RHINOVIRUS RECEPTOR / CELL ADHESION / INTEGRIN LIGAND / GLYCOPROTEIN / LFA-1 LIGAND / IMMUNOGLOBULIN FOLD / TRANSMEMBRANE | ||||||
Function / homology | Function and homology information regulation of leukocyte mediated cytotoxicity / T cell extravasation / positive regulation of cellular extravasation / regulation of ruffle assembly / T cell antigen processing and presentation / T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell / membrane to membrane docking / adhesion of symbiont to host / establishment of endothelial barrier / cell adhesion mediated by integrin ...regulation of leukocyte mediated cytotoxicity / T cell extravasation / positive regulation of cellular extravasation / regulation of ruffle assembly / T cell antigen processing and presentation / T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell / membrane to membrane docking / adhesion of symbiont to host / establishment of endothelial barrier / cell adhesion mediated by integrin / heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules / leukocyte cell-cell adhesion / leukocyte migration / Interleukin-10 signaling / immunological synapse / Integrin cell surface interactions / negative regulation of endothelial cell apoptotic process / negative regulation of extrinsic apoptotic signaling pathway via death domain receptors / cellular response to leukemia inhibitory factor / cellular response to glucose stimulus / cellular response to amyloid-beta / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / Interferon gamma signaling / transmembrane signaling receptor activity / integrin binding / virus receptor activity / signaling receptor activity / collagen-containing extracellular matrix / Interleukin-4 and Interleukin-13 signaling / receptor-mediated virion attachment to host cell / positive regulation of ERK1 and ERK2 cascade / cell adhesion / membrane raft / external side of plasma membrane / focal adhesion / cell surface / extracellular space / extracellular exosome / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT, MIR, PHASE RECOMBINATION / Resolution: 2.1 Å | ||||||
Authors | Bella, J. / Kolatkar, P.R. / Marlor, C. / Greve, J.M. / Rossmann, M.G. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 1998 Title: The structure of the two amino-terminal domains of human ICAM-1 suggests how it functions as a rhinovirus receptor and as an LFA-1 integrin ligand. Authors: Bella, J. / Kolatkar, P.R. / Marlor, C.W. / Greve, J.M. / Rossmann, M.G. #1: Journal: Proc.Natl.Acad.Sci.USA / Year: 1993 Title: Structure of a Human Rhinovirus Complexed with its Receptor Molecule Authors: Olson, N.H. / Kolatkar, P.R. / Oliveira, M.A. / Cheng, R.H. / Greve, J.M. / Mcclelland, A. / Baker, T.S. / Rossmann, M.G. #2: Journal: Cell(Cambridge,Mass.) / Year: 1989 Title: The Major Human Rhinovirus Receptor is Icam-1 Authors: Greve, J.M. / Davis, G. / Meyer, A.M. / Forte, C.P. / Yost, S.C. / Marlor, C.W. / Kamarck, M.E. / Mcclelland, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1iam.cif.gz | 49.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1iam.ent.gz | 37.8 KB | Display | PDB format |
PDBx/mmJSON format | 1iam.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ia/1iam ftp://data.pdbj.org/pub/pdb/validation_reports/ia/1iam | HTTPS FTP |
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-Related structure data
Related structure data | 1zxqS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 20480.338 Da / Num. of mol.: 1 / Fragment: TWO N-TERMINAL, IMMUNOGLOBULIN DOMAINS / Mutation: N103Q, N118Q, N156Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Cell: LEUKOCYTES, ENDOTHELIAL CELLS / Cell line: SF9 / Plasmid: PVL1393 (PHARMINGEN) / Cell line (production host): SF9 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P05362 |
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#2: Sugar | ChemComp-NAG / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.65 Å3/Da / Density % sol: 53.56 % Description: THERE IS A SIGNIFICANT NUMBER OF OVERLOADS IN THE LOWER RESOLUTION SHELLS (19% BETWEEN 20 AN 5 ANGSTROM). DATA WAS SCALED WITH THE "INCLUDE OVERLOADS" OPTION IN SCALEPACK. LOW RESOLUTION ...Description: THERE IS A SIGNIFICANT NUMBER OF OVERLOADS IN THE LOWER RESOLUTION SHELLS (19% BETWEEN 20 AN 5 ANGSTROM). DATA WAS SCALED WITH THE "INCLUDE OVERLOADS" OPTION IN SCALEPACK. LOW RESOLUTION DATA BETWEEN 20 AND 5 ANGSTROM HAS NOT BEEN USED IN THE REFINEMENT. | ||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.5 Details: PROTEIN IN 10 MM TRIS, PH 7.5, 25 MM NACL, WAS CRYSTALLIZED FROM 20% PEG 4000 IN 10 MM TRIS AS PRECIPITANT | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 120 K |
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Diffraction source | Source: SYNCHROTRON / Site: CHESS / Beamline: A1 / Wavelength: 0.92 |
Detector | Detector: CCD / Date: Feb 26, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→20 Å / Num. obs: 10629 / % possible obs: 81.1 % / Observed criterion σ(I): 2 / Redundancy: 3 % / Biso Wilson estimate: 25.1 Å2 / Rmerge(I) obs: 0.036 / Net I/σ(I): 24.15 |
Reflection shell | Resolution: 2.1→2.17 Å / Redundancy: 0.3 % / Rmerge(I) obs: 0.059 / Mean I/σ(I) obs: 10.21 / % possible all: 33.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT, MIR, PHASE RECOMBINATION Starting model: ICAM-2, PDB ENTRY 1ZXQ Resolution: 2.1→5 Å / Rfactor Rfree error: 0.01 / Data cutoff high absF: 100000 / Data cutoff low absF: 0.001 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 24.6 Å2
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Refinement step | Cycle: LAST / Resolution: 2.1→5 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.1→2.19 Å / Rfactor Rfree error: 0.037 / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.851 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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