+Open data
-Basic information
Entry | Database: PDB / ID: 1hyq | ||||||
---|---|---|---|---|---|---|---|
Title | MIND BACTERIAL CELL DIVISION REGULATOR FROM A. FULGIDUS | ||||||
Components | CELL DIVISION INHIBITOR (MIND-1) | ||||||
Keywords | CELL CYCLE / MinC / FtsZ / bacterial cell division | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Archaeoglobus fulgidus (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.6 Å | ||||||
Authors | Cordell, S.C. / Lowe, J. | ||||||
Citation | Journal: FEBS Lett. / Year: 2001 Title: Crystal structure of the bacterial cell division regulator MinD. Authors: Cordell, S.C. / Lowe, J. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1hyq.cif.gz | 53.5 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1hyq.ent.gz | 39 KB | Display | PDB format |
PDBx/mmJSON format | 1hyq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hy/1hyq ftp://data.pdbj.org/pub/pdb/validation_reports/hy/1hyq | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 27689.512 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Archaeoglobus fulgidus (archaea) / Gene: AF0696 / Plasmid: PHIS17 / Production host: Escherichia coli (E. coli) / Strain (production host): C41 / References: UniProt: O29562 |
---|---|
#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 3.07 Å3/Da / Density % sol: 59.9 % | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.6 Details: 2% PEG6000, 100mM TRIS buffer pH 8.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K | ||||||||||||||||||||
Crystal grow | *PLUS Temperature: 19 ℃ | ||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-4 / Wavelength: 0.97900, 0.97920, 0.93930 | ||||||||||||
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Oct 1, 2000 | ||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
| ||||||||||||
Reflection | Resolution: 2.6→35 Å / Num. all: 11094 / Num. obs: 10622 / % possible obs: 95.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.3 % / Biso Wilson estimate: 60.81 Å2 / Rmerge(I) obs: 0.071 / Net I/σ(I): 12.2 | ||||||||||||
Reflection shell | Resolution: 2.6→2.74 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.281 / Mean I/σ(I) obs: 3.8 / % possible all: 98.6 | ||||||||||||
Reflection shell | *PLUS % possible obs: 98.6 % |
-Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MAD / Resolution: 2.6→35 Å / Isotropic thermal model: isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
| ||||||||||||||||||||
Displacement parameters | Biso mean: 52 Å2
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→35 Å
| ||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||
LS refinement shell | Resolution: 2.6→2.69 Å
|