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Yorodumi- PDB-1efx: STRUCTURE OF A COMPLEX BETWEEN THE HUMAN NATURAL KILLER CELL RECE... -
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-Basic information
Entry | Database: PDB / ID: 1efx | ||||||
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Title | STRUCTURE OF A COMPLEX BETWEEN THE HUMAN NATURAL KILLER CELL RECEPTOR KIR2DL2 AND A CLASS I MHC LIGAND HLA-CW3 | ||||||
Components |
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Keywords | IMMUNE SYSTEM / MHC / HLA / class I / KIR / NK cell receptor / Immunoglobulin fold / receptor-MHC complex | ||||||
Function / homology | Function and homology information Sensing of DNA Double Strand Breaks / regulation of DNA recombination / entry of viral genome into host nucleus through nuclear pore complex via importin / positive regulation of viral life cycle / NS1 Mediated Effects on Host Pathways / NLS-dependent protein nuclear import complex / host cell / nuclear import signal receptor activity / CREB1 phosphorylation through the activation of CaMKII/CaMKK/CaMKIV cascasde / nuclear localization sequence binding ...Sensing of DNA Double Strand Breaks / regulation of DNA recombination / entry of viral genome into host nucleus through nuclear pore complex via importin / positive regulation of viral life cycle / NS1 Mediated Effects on Host Pathways / NLS-dependent protein nuclear import complex / host cell / nuclear import signal receptor activity / CREB1 phosphorylation through the activation of CaMKII/CaMKK/CaMKIV cascasde / nuclear localization sequence binding / DNA metabolic process / CaMK IV-mediated phosphorylation of CREB / NLS-bearing protein import into nucleus / TAP binding / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent / antigen processing and presentation of endogenous peptide antigen via MHC class Ib / secretory granule membrane / positive regulation of ferrous iron binding / positive regulation of transferrin receptor binding / early endosome lumen / positive regulation of receptor binding / Nef mediated downregulation of MHC class I complex cell surface expression / DAP12 interactions / negative regulation of receptor binding / lumenal side of endoplasmic reticulum membrane / Endosomal/Vacuolar pathway / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / cellular response to iron(III) ion / negative regulation of forebrain neuron differentiation / ER to Golgi transport vesicle membrane / response to molecule of bacterial origin / regulation of erythrocyte differentiation / regulation of iron ion transport / MHC class I peptide loading complex / HFE-transferrin receptor complex / T cell mediated cytotoxicity / ISG15 antiviral mechanism / cellular response to iron ion / antigen processing and presentation of endogenous peptide antigen via MHC class I / positive regulation of T cell cytokine production / MHC class I protein complex / multicellular organismal-level iron ion homeostasis / positive regulation of T cell mediated cytotoxicity / peptide antigen assembly with MHC class II protein complex / negative regulation of neurogenesis / MHC class II protein complex / positive regulation of receptor-mediated endocytosis / protein import into nucleus / cellular response to nicotine / histone deacetylase binding / recycling endosome membrane / phagocytic vesicle membrane / specific granule lumen / peptide antigen binding / SARS-CoV-1 activates/modulates innate immune responses / positive regulation of cellular senescence / antigen processing and presentation of exogenous peptide antigen via MHC class II / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / Interferon gamma signaling / positive regulation of immune response / negative regulation of epithelial cell proliferation / Modulation by Mtb of host immune system / positive regulation of T cell activation / Interferon alpha/beta signaling / sensory perception of smell / negative regulation of neuron projection development / tertiary granule lumen / DAP12 signaling / MHC class II protein complex binding / late endosome membrane / T cell differentiation in thymus / positive regulation of protein binding / ER-Phagosome pathway / iron ion transport / protein refolding / early endosome membrane / protein homotetramerization / nuclear membrane / Estrogen-dependent gene expression / intracellular iron ion homeostasis / amyloid fibril formation / adaptive immune response / learning or memory / immune response / Amyloid fiber formation / lysosomal membrane / endoplasmic reticulum lumen / external side of plasma membrane / Golgi membrane / signaling receptor binding / focal adhesion / innate immune response / Neutrophil degranulation / endoplasmic reticulum membrane / SARS-CoV-2 activates/modulates innate and adaptive immune responses / Golgi apparatus / cell surface / endoplasmic reticulum / protein homodimerization activity Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3 Å | ||||||
Authors | Boyington, J.C. / Motyka, S.A. / Schuck, P. / Brooks, A.G. / Sun, P.D. | ||||||
Citation | Journal: Nature / Year: 2000 Title: Crystal structure of an NK cell immunoglobulin-like receptor in complex with its class I MHC ligand. Authors: Boyington, J.C. / Motyka, S.A. / Schuck, P. / Brooks, A.G. / Sun, P.D. #1: Journal: Proc.Natl.Acad.Sci.USA / Year: 1999 Title: Crystal Structure of the HLA-Cw3 Allotype-specific Killer Cell Inhibitory Receptor KIR2DL2 Authors: Snyder, G.A. / Brooks, A.G. / Sun, P.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1efx.cif.gz | 157.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1efx.ent.gz | 125.1 KB | Display | PDB format |
PDBx/mmJSON format | 1efx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ef/1efx ftp://data.pdbj.org/pub/pdb/validation_reports/ef/1efx | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | The biological assembly is the 1:1 complex between KIR2DL2 and HLA-Cw3 observed in the asymmetric unit The biological assembly is the 1:1 complex between KIR2DL2 and HLA-Cw3 observed in the asymmetric unit |
-Components
#1: Protein | Mass: 32185.391 Da / Num. of mol.: 1 Fragment: EXTRACELLULAR ALPHA-1, ALPHA-2 AND ALPHA-3 DOMAINS Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: MODIFIED PET30A / Production host: Escherichia coli (E. coli) / References: GenBank: 495038, UniProt: P10321*PLUS | ||
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#2: Protein | Mass: 11879.356 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: MATURE FORM / Source: (gene. exp.) Homo sapiens (human) / Plasmid: MODIFIED PET30A / Production host: Escherichia coli (E. coli) / References: UniProt: P61769 | ||
#3: Protein/peptide | Mass: 868.029 Da / Num. of mol.: 1 / Fragment: RESIDUES 204-212 / Source method: obtained synthetically Details: This peptide was chemically synthesized. The sequence is naturally found in homo sapiens (human). References: UniProt: P52292 | ||
#4: Protein | Mass: 22050.682 Da / Num. of mol.: 2 / Fragment: EXTRACELLULAR D1 AND D2 DOMAINS Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: MODIFIED PET30A / Production host: Escherichia coli (E. coli) / References: UniProt: P43627 #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.64 Å3/Da / Density % sol: 65.9 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: PEG 20000, calcium chloride, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 93 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X9B / Wavelength: 1.0358 |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jul 25, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0358 Å / Relative weight: 1 |
Reflection | Resolution: 3→30 Å / Num. all: 25779 / Num. obs: 24517 / % possible obs: 92.1 % / Observed criterion σ(I): -0.5 / Redundancy: 4.2 % / Biso Wilson estimate: 63.6 Å2 / Rmerge(I) obs: 0.073 / Net I/σ(I): 15.6 |
Reflection shell | Resolution: 3→3.11 Å / Redundancy: 2.2 % / Rmerge(I) obs: 0.327 / Mean I/σ(I) obs: 2 / Num. unique all: 2321 / % possible all: 75.4 |
Reflection shell | *PLUS % possible obs: 75.4 % / Num. unique obs: 1976 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: KIR2DL2 (PDB ENTRY 2DL2) and HLA-A2 (PDB ENTRY 1B0G) Resolution: 3→10 Å / Rfactor Rfree error: 0.009 / Data cutoff high absF: 2163178.14 / Data cutoff high rms absF: 2163178.14 / Data cutoff low absF: 0 / Isotropic thermal model: GROUP / Cross valid method: THROUGHOUT / σ(F): 1 / Stereochemistry target values: Engh & Huber / Details: Bulk solvent model used
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Solvent computation | Solvent model: flat model / Bsol: 42.0521 Å2 / ksol: 0.28471 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 63.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3→3.18 Å / Rfactor Rfree error: 0.039 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.9 / Classification: refinement | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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