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Yorodumi- PDB-1e17: Solution structure of the DNA binding domain of the human Forkhea... -
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-Basic information
Entry | Database: PDB / ID: 1.0E+17 | ||||||
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Title | Solution structure of the DNA binding domain of the human Forkhead transcription factor AFX (FOXO4) | ||||||
Components | AFX | ||||||
Keywords | DNA BINDING DOMAIN / WINGED HELIX | ||||||
Function / homology | Function and homology information negative regulation of smooth muscle cell differentiation / FOXO-mediated transcription of cell cycle genes / AKT phosphorylates targets in the nucleus / response to water-immersion restraint stress / negative regulation of G0 to G1 transition / Cardiogenesis / muscle organ development / Regulation of FOXO transcriptional activity by acetylation / FOXO-mediated transcription of cell death genes / positive regulation of smooth muscle cell migration ...negative regulation of smooth muscle cell differentiation / FOXO-mediated transcription of cell cycle genes / AKT phosphorylates targets in the nucleus / response to water-immersion restraint stress / negative regulation of G0 to G1 transition / Cardiogenesis / muscle organ development / Regulation of FOXO transcriptional activity by acetylation / FOXO-mediated transcription of cell death genes / positive regulation of smooth muscle cell migration / Constitutive Signaling by AKT1 E17K in Cancer / mitotic G2 DNA damage checkpoint signaling / Regulation of localization of FOXO transcription factors / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / negative regulation of angiogenesis / response to nutrient levels / promoter-specific chromatin binding / stem cell differentiation / beta-catenin binding / sequence-specific double-stranded DNA binding / insulin receptor signaling pathway / DNA-binding transcription activator activity, RNA polymerase II-specific / response to oxidative stress / DNA-binding transcription factor binding / sequence-specific DNA binding / nucleic acid binding / DNA-binding transcription factor activity, RNA polymerase II-specific / Ub-specific processing proteases / nuclear speck / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of cell population proliferation / chromatin / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / enzyme binding / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / nucleus / identical protein binding / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | SOLUTION NMR / SIMULATED ANNEALING IN TORSION ANGLE SPACE | ||||||
Authors | Weigelt, J. / Climent, I. / Dahlman-Wright, K. / Wikstrom, M. | ||||||
Citation | Journal: J.Biomol.NMR / Year: 2000 Title: 1H, 13C and 15N Resonance Assignments of the DNA Binding Domain of the Human Forkhead Transcription Factor Afx Authors: Weigelt, J. / Climent, I. / Dahlman-Wright, K. / Wikstrom, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1e17.cif.gz | 561.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1e17.ent.gz | 465.8 KB | Display | PDB format |
PDBx/mmJSON format | 1e17.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/e1/1e17 ftp://data.pdbj.org/pub/pdb/validation_reports/e1/1e17 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 16611.670 Da / Num. of mol.: 1 / Fragment: DNA BINDING DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Cellular location: NUCLEUS & CYTOPLASM / Plasmid: PET15B / Cellular location (production host): CYTOPLASM / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P98177 |
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Sequence details | GLY 58 - PRO 81 IN PDB ENTRY BELONG TO ARTIFICIAL |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR details | Text: EXPERIMENTAL DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE |
-Sample preparation
Details | Contents: 10% WATER/90% D2O |
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Sample conditions | Ionic strength: 100 mM / pH: 6.3 / Pressure: 1 atm / Temperature: 304 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: SIMULATED ANNEALING IN TORSION ANGLE SPACE / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | ||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST X-PLOR ENERGY / Conformers calculated total number: 85 / Conformers submitted total number: 20 |