+Open data
-Basic information
Entry | Database: PDB / ID: 1buj | ||||||
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Title | STRUCTURE OF BINASE IN SOLUTION | ||||||
Components | PROTEIN (BINASE) | ||||||
Keywords | HYDROLASE / MICROBIAL RIBONUCLEASE | ||||||
Function / homology | Function and homology information Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters / RNA endonuclease activity / RNA binding / extracellular region Similarity search - Function | ||||||
Biological species | Bacillus intermedius (bacteria) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Reibarkh, M.Y. / Nolde, D.E. / Vasilieva, L.I. / Arseniev, A.S. | ||||||
Citation | Journal: FEBS Lett. / Year: 1998 Title: Three-dimensional structure of binase in solution. Authors: Reibarkh, M.Y.a. / Nolde, D.E. / Vasilieva, L.I. / Bocharov, E.V. / Shulga, A.A. / Kirpichnikov, M.P. / Arseniev, A.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1buj.cif.gz | 656.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1buj.ent.gz | 571.3 KB | Display | PDB format |
PDBx/mmJSON format | 1buj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bu/1buj ftp://data.pdbj.org/pub/pdb/validation_reports/bu/1buj | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12227.623 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: G-SPECIFIC ENDORIBONUCLEASE / Source: (gene. exp.) Bacillus intermedius (bacteria) / Strain: 7P / Plasmid: PGEMEX1 / Gene (production host): BINASE / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)(PLYSS) References: UniProt: P00649, Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Sample conditions | Ionic strength: 0.04 / pH: 6.7 / Pressure: 1 atm / Temperature: 303 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITY / Manufacturer: Varian / Model: UNITY / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE | ||||||||||||
NMR ensemble | Conformer selection criteria: LEAST RESTRAINT VIOLATION / Conformers submitted total number: 20 |