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Yorodumi- PDB-1bmw: A fibronectin type III fold in plant allergens: The solution stru... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1bmw | ||||||
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Title | A fibronectin type III fold in plant allergens: The solution structure of Phl PII from timothy grass pollen, NMR, 38 STRUCTURES | ||||||
Components | POLLEN ALLERGEN PHL P2 | ||||||
Keywords | ALLERGEN / ALLERGY / IMMUNOGLOBULINS / IMMUNOLOGY | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Phleum pratense (timothy grass) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | De Marino, S. / Morelli, M.A.C. / Fraternali, F. / Tamborino, E. / Vrtala, S. / Dolecek, C. / Arosio, P. / Valenta, R. / Pastore, A. | ||||||
Citation | Journal: Structure Fold.Des. / Year: 1999 Title: An Immunoglobulin-Like Fold in a Major Plant Allergen: The Solution Structure of Phl P 2 from Timothy Grass Pollen. Authors: De Marino, S. / Morelli, M.A. / Fraternali, F. / Tamborino, E. / Vrtala, S. / Dolocek, C. / Arosio, P. / Valenta, R. / Pastore, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1bmw.cif.gz | 1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1bmw.ent.gz | 936.1 KB | Display | PDB format |
PDBx/mmJSON format | 1bmw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bm/1bmw ftp://data.pdbj.org/pub/pdb/validation_reports/bm/1bmw | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10828.056 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Phleum pratense (timothy grass) / Cell line: BL21 / Plasmid: PMW 172 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P43214 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURE WAS DETERMINED USING 2D HOMONUCLEAR NMR SPECTROSCOPY |
-Sample preparation
Details | Contents: H2O |
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Sample conditions | Ionic strength: 10 mM PHOSPHATE BUFFER / pH: 7 / Pressure: 1 atm / Temperature: 300 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker AMX600 / Manufacturer: Bruker / Model: AMX600 / Field strength: 600 MHz |
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-Processing
Software |
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NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: ALSO USED ARIA, AUTHOR NILGES, SIMULATED ANNEALING PROTOCOL WHICH PROGRESSIVELY INCLUDES, BY AN ITERATIVE PROCEDURE, DISTANCE RESTRAINTS DIRECTLY ASSIGNED ON THE BASIS OF CHEMICAL SHIFT AND NOE INTENSITY LISTS. | ||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST ENERGY / Conformers calculated total number: 50 / Conformers submitted total number: 38 |