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Yorodumi- PDB-1bbg: RAGWEED POLLEN ALLERGEN FROM AMBROSIA TRIFIDA V, NMR, MINIMIZED A... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1bbg | ||||||
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Title | RAGWEED POLLEN ALLERGEN FROM AMBROSIA TRIFIDA V, NMR, MINIMIZED AVERAGE STRUCTURE | ||||||
Components | POLLEN ALLERGEN 5 | ||||||
Keywords | ALLERGEN / PROTEIN ALLERGEN / SMALL HIGHLY DISULFIDE BONDED | ||||||
Function / homology | Amb V Allergen / Amb V Allergen / Amb V Allergen superfamily / Ragweed group 5 pollen allergen / Amb V Allergen / Double Stranded RNA Binding Domain / 2-Layer Sandwich / Alpha Beta / Pollen allergen Amb t 5 Function and homology information | ||||||
Biological species | Ambrosia trifida (giant ragweed) | ||||||
Method | SOLUTION NMR / ITERATIVE SIMULATED ANNEALING | ||||||
Authors | Warren, G.L. / Tuner, C.J. / Petsko, G.A. / Brunger, A.T. | ||||||
Citation | Journal: To be Published Title: A Highly Precise Solution 1H NMR Structure of Ragweed Allergen Amb. T. V Authors: Warren, G.L. / Turner, C.J. / Petsko, G.A. / Brunger, A.T. #1: Journal: Biochemistry / Year: 1992 Title: Determination of the Three-Dimensional Solution Structure of Ragweed Allergen Amb T V by Nuclear Magnetic Resonance Spectroscopy Authors: Metzler, W.J. / Valentine, K. / Roebber, M. / Friedrichs, M.S. / Marsh, D.G. / Mueller, L. #2: Journal: Mol.Immunol. / Year: 1985 Title: Ra5G, a Homologue of Ra5 in Giant Ragweed Pollen: Isolation, Hla-Dr-Associated Activity and Amino Acid Sequence Authors: Goodfriend, L. / Choudhury, A.M. / Klapper, D.G. / Coulter, K.M. / Dorval, G. / Del Carpio, J. / Osterland, C.K. #3: Journal: Biochemistry / Year: 1985 Title: 500-Mhz 1H NMR Studies of Ragweed Allergen Ra5 Authors: Vidusek, D.A. / Roberts, M.F. / Goodfriend, L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1bbg.cif.gz | 19.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1bbg.ent.gz | 14.1 KB | Display | PDB format |
PDBx/mmJSON format | 1bbg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bb/1bbg ftp://data.pdbj.org/pub/pdb/validation_reports/bb/1bbg | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 4325.987 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: POLLEN / Source: (natural) Ambrosia trifida (giant ragweed) / References: UniProt: P10414 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THIS IS A MINIMIZED AVERAGE STRUCTURE. THE STRUCTURE WAS DETERMINING USING HOMONUCLEAR 2- AND 3-D NMR SPECTROSCOPY |
-Sample preparation
Sample conditions | pH: 4.0 / Temperature: 293 K |
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-NMR measurement
NMR spectrometer |
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-Processing
Software |
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NMR software |
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Refinement | Method: ITERATIVE SIMULATED ANNEALING / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE MANUSCRIPT SUBMITTED TO JMB ABOVE. | ||||||||||||||||
NMR ensemble | Conformer selection criteria: BONDS RMS < 0.01, ANGLE RMS < 1.0, IMPROPER RMS < 1.0, 0 NOE VIOL > 0.5, 0 DIHEDRAL VIOL > 5.0 Conformers calculated total number: 30 / Conformers submitted total number: 1 |