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Yorodumi- PDB-1b3i: NMR SOLUTION STRUCTURE OF PLASTOCYANIN FROM THE PHOTOSYNTHETIC PR... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1b3i | ||||||
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Title | NMR SOLUTION STRUCTURE OF PLASTOCYANIN FROM THE PHOTOSYNTHETIC PROKARYOTE, PROCHLOROTHRIX HOLLANDICA (MINIMIZED AVERAGE STRUCTURE) | ||||||
Components | PROTEIN (PLASTOCYANIN) | ||||||
Keywords | ELECTRON TRANSPORT / TYPE I COPPER PROTEIN / PHOTOSYNTHESIS | ||||||
Function / homology | Function and homology information plasma membrane-derived thylakoid membrane / electron transfer activity / copper ion binding Similarity search - Function | ||||||
Biological species | Prochlorothrix hollandica (bacteria) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Babu, C.R. / Volkman, B.F. / Bullerjahn, G.S. | ||||||
Citation | Journal: Biochemistry / Year: 1999 Title: NMR solution structure of plastocyanin from the photosynthetic prokaryote, Prochlorothrix hollandica. Authors: Babu, C.R. / Volkman, B.F. / Bullerjahn, G.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1b3i.cif.gz | 40.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1b3i.ent.gz | 27.1 KB | Display | PDB format |
PDBx/mmJSON format | 1b3i.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b3/1b3i ftp://data.pdbj.org/pub/pdb/validation_reports/b3/1b3i | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10148.560 Da / Num. of mol.: 1 / Mutation: T2S Source method: isolated from a genetically manipulated source Details: T2S MUTATION INTRODUCED TO CLONE IN EXPRESSION VECTOR Source: (gene. exp.) Prochlorothrix hollandica (bacteria) / Description: INCLUSION BODIES WERE REFOLDED IN-VITRO / Cellular location: THYLAKOID LUMENThylakoid / Gene: PETE / Plasmid: PVAPC10 Cellular location (production host): CYTOPLASM - INCLUSION BODIES Gene (production host): PETE / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)PLYSS / References: UniProt: P50057, EC: 1.10.99.1 |
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#2: Chemical | ChemComp-CU1 / |
Sequence details | T2S - INTRODUCED |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: MINIMIZED AVERAGE STRUCTURE. THE STRUCTURE WAS DETERMINED USING TWO- DIMENCIONAL NMR SPECTROSCOPY. WATER SUPPRESSION WAS ACHIEVED WITH A WATERGATE SEQ. WITH A 3-9-19 SELECTIVE INVERSION. |
-Sample preparation
Details | Contents: 90% WATER/10% D2O |
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Sample conditions | Ionic strength: 20mM POTASSIUM PHOSPHATE / pH: 7.0 / Pressure: 1 atm / Temperature: 298 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DMX750 / Manufacturer: Bruker / Model: DMX750 / Field strength: 750 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 Details: REFINEMENT DETAILS ARE IN THE SUBMITTED FILE AND IN THE JRNL CITATION ABOVE. THE RESTRAINTS ARE IN THE FILES WITH THE 19 CONFORMERS. | ||||||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST TARGET FUNCTION / Conformers calculated total number: 40 / Conformers submitted total number: 1 |