+Open data
-Basic information
Entry | Database: PDB / ID: 1a44 | ||||||
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Title | PHOSPHATIDYLETHANOLAMINE BINDING PROTEIN FROM CALF BRAIN | ||||||
Components | PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN | ||||||
Keywords | LIPID BINDING PROTEIN / LIPID-BINDING / LIPID-BINDING PROTEIN | ||||||
Function / homology | Function and homology information MAP2K and MAPK activation / Negative regulation of MAPK pathway / negative regulation of MAPK cascade / serine-type endopeptidase inhibitor activity / lipid binding / ATP binding / cytoplasm Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIR / Resolution: 1.84 Å | ||||||
Authors | Serre, L. / Vallee, B. / Zelwer, C. / Schoentgen, F. | ||||||
Citation | Journal: Structure / Year: 1998 Title: Crystal structure of the phosphatidylethanolamine-binding protein from bovine brain: a novel structural class of phospholipid-binding proteins. Authors: Serre, L. / Vallee, B. / Bureaud, N. / Schoentgen, F. / Zelwer, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1a44.cif.gz | 53.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1a44.ent.gz | 37.9 KB | Display | PDB format |
PDBx/mmJSON format | 1a44.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a4/1a44 ftp://data.pdbj.org/pub/pdb/validation_reports/a4/1a44 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 20754.363 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: CALF / Source: (natural) Bos taurus (cattle) / Cellular location: CYTOPLASM / Organ: BRAIN / References: UniProt: P13696 |
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#2: Chemical | ChemComp-ACT / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.99 Å3/Da / Density % sol: 38 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 4.6 / Details: pH 4.6 | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: LURE / Beamline: DW32 / Wavelength: 0.97 |
Detector | Date: Nov 1, 1997 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 1.84→36.04 Å / Num. obs: 14517 / % possible obs: 99.8 % / Observed criterion σ(I): 1 / Redundancy: 3.3 % / Biso Wilson estimate: 9.742 Å2 / Rsym value: 0.039 / Net I/σ(I): 15.8 |
Reflection shell | Resolution: 1.84→1.94 Å / Redundancy: 3.3 % / Mean I/σ(I) obs: 8 / Rsym value: 0.093 / % possible all: 98.6 |
Reflection | *PLUS Num. measured all: 48585 / Rmerge(I) obs: 0.039 |
Reflection shell | *PLUS Rmerge(I) obs: 0.095 |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 1.84→36 Å / Cross valid method: RFREE / σ(F): 0
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Displacement parameters | Biso mean: 11.89 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.84→36 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 32 Å / Rfactor obs: 0.147 / Rfactor Rfree: 0.212 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |