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Yorodumi- PDB-1a11: NMR STRUCTURE OF MEMBRANE SPANNING SEGMENT 2 OF THE ACETYLCHOLINE... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1a11 | ||||||
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Title | NMR STRUCTURE OF MEMBRANE SPANNING SEGMENT 2 OF THE ACETYLCHOLINE RECEPTOR IN DPC MICELLES, 10 STRUCTURES | ||||||
Components | ACETYLCHOLINE RECEPTOR M2 | ||||||
Keywords | ACETYLCHOLINE RECEPTOR / M2 / MICELLE | ||||||
Function / homology | Function and homology information Highly sodium permeable postsynaptic acetylcholine nicotinic receptors / skeletal muscle tissue growth / musculoskeletal movement / acetylcholine-gated channel complex / acetylcholine-gated monoatomic cation-selective channel activity / postsynaptic specialization membrane / acetylcholine binding / ligand-gated monoatomic ion channel activity / monoatomic cation transport / skeletal muscle contraction ...Highly sodium permeable postsynaptic acetylcholine nicotinic receptors / skeletal muscle tissue growth / musculoskeletal movement / acetylcholine-gated channel complex / acetylcholine-gated monoatomic cation-selective channel activity / postsynaptic specialization membrane / acetylcholine binding / ligand-gated monoatomic ion channel activity / monoatomic cation transport / skeletal muscle contraction / regulation of membrane potential / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / neuromuscular junction / transmembrane signaling receptor activity / neuron projection / synapse / plasma membrane Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | SOLUTION NMR / DISTANCE GEOMETRY, SIMULATED ANNEALING | ||||||
Authors | Gesell, J.J. / Sun, W. / Montal, M. / Opella, S.J. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1999 Title: Structures of the M2 channel-lining segments from nicotinic acetylcholine and NMDA receptors by NMR spectroscopy. Authors: Opella, S.J. / Marassi, F.M. / Gesell, J.J. / Valente, A.P. / Kim, Y. / Oblatt-Montal, M. / Montal, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1a11.cif.gz | 79 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1a11.ent.gz | 57.2 KB | Display | PDB format |
PDBx/mmJSON format | 1a11.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a1/1a11 ftp://data.pdbj.org/pub/pdb/validation_reports/a1/1a11 | HTTPS FTP |
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-Related structure data
Related structure data | 1cekC 1eq8C 2nr1C C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 2666.143 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Organ: BRAIN / Plasmid: PGEX FUSION / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P25110 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Sample conditions | pH: 5.5 / Temperature: 313 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
Software |
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NMR software |
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Refinement | Method: DISTANCE GEOMETRY, SIMULATED ANNEALING / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | ||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST ENERGY / Conformers calculated total number: 30 / Conformers submitted total number: 10 |