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Yorodumi- PDB-4cln: STRUCTURE OF A RECOMBINANT CALMODULIN FROM DROSOPHILA MELANOGASTE... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4cln | ||||||
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Title | STRUCTURE OF A RECOMBINANT CALMODULIN FROM DROSOPHILA MELANOGASTER REFINED AT 2.2-ANGSTROMS RESOLUTION | ||||||
Components | CALMODULIN | ||||||
Keywords | CALCIUM BINDING PROTEIN | ||||||
Function / homology | Function and homology information metarhodopsin inactivation / myosin VI complex / myosin VI head/neck binding / myosin VII complex / photoreceptor cell axon guidance / negative regulation of opsin-mediated signaling pathway / rhabdomere / rhabdomere development / myosin V complex / : ...metarhodopsin inactivation / myosin VI complex / myosin VI head/neck binding / myosin VII complex / photoreceptor cell axon guidance / negative regulation of opsin-mediated signaling pathway / rhabdomere / rhabdomere development / myosin V complex / : / kinetochore organization / : / actin filament-based movement / rhodopsin mediated signaling pathway / Neutrophil degranulation / myosin V binding / channel regulator activity / cellular response to ethanol / muscle cell cellular homeostasis / myosin heavy chain binding / mitotic spindle pole / centriole replication / enzyme regulator activity / centriole / sensory perception of sound / mitotic spindle / spindle / sensory perception of smell / cell cortex / midbody / protein phosphorylation / centrosome / calcium ion binding / nucleoplasm / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.2 Å | ||||||
Authors | Taylor, D.A. / Sack, J.S. / Maune, J.F. / Beckingham, K. / Quiocho, F.A. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 1991 Title: Structure of a recombinant calmodulin from Drosophila melanogaster refined at 2.2-A resolution. Authors: Taylor, D.A. / Sack, J.S. / Maune, J.F. / Beckingham, K. / Quiocho, F.A. #1: Journal: J.Mol.Biol. / Year: 1987 Title: Structure and Sequence of the Drosophila Melanogaster Calmodulin Gene Authors: Smith, V.L. / Doyle, K.E. / Maune, J.F. / Munjaal, R.P. / Beckingham, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4cln.cif.gz | 44.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4cln.ent.gz | 30.9 KB | Display | PDB format |
PDBx/mmJSON format | 4cln.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cl/4cln ftp://data.pdbj.org/pub/pdb/validation_reports/cl/4cln | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 16694.324 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / References: UniProt: P62152 | ||
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#2: Chemical | ChemComp-CA / #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.53 % | ||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 18 ℃ / Method: vapor diffusion, hanging drop / pH: 4 / Details: initial seeding crystal | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 20 Å / Num. obs: 6831 / % possible obs: 81.7 % / Observed criterion σ(F): 2 / Num. measured all: 10617 / Rmerge F obs: 0.068 |
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-Processing
Software | Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 2.2→10 Å / σ(F): 2 /
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Refinement step | Cycle: LAST / Resolution: 2.2→10 Å
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Refine LS restraints |
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Software | *PLUS Name: 'X-PLOR, PROLSQ' / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 10 Å / Num. reflection obs: 5239 / σ(F): 2 / Rfactor obs: 0.197 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |