+Open data
-Basic information
Entry | Database: PDB / ID: 4ch1 | ||||||
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Title | RRM domain from C. elegans SUP-12 bound to GGTGTGC DNA | ||||||
Components |
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Keywords | TRANSCRIPTION/DNA / TRANSCRIPTION-DNA COMPLEX / MUSCLE / DEVELOPMENT | ||||||
Function / homology | Function and homology information alternative mRNA splicing, via spliceosome / pre-mRNA binding / regulation of locomotion / regulation of alternative mRNA splicing, via spliceosome / pre-mRNA intronic binding / regulation of actin cytoskeleton organization / nuclear speck / ribonucleoprotein complex Similarity search - Function | ||||||
Biological species | CAENORHABDITIS ELEGANS (invertebrata) | ||||||
Method | SOLUTION NMR / ARIA1.2 | ||||||
Authors | Amrane, S. / Mackereth, C.D. | ||||||
Citation | Journal: Nat.Commun. / Year: 2014 Title: Backbone-Independent Nucleic Acid Binding by Splicing Factor Sup-12 Reveals Key Aspects of Molecular Recognition Authors: Amrane, S. / Rebora, K. / Zniber, I. / Dupuy, D. / Mackereth, C.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4ch1.cif.gz | 555.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4ch1.ent.gz | 485.8 KB | Display | PDB format |
PDBx/mmJSON format | 4ch1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ch/4ch1 ftp://data.pdbj.org/pub/pdb/validation_reports/ch/4ch1 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10831.196 Da / Num. of mol.: 1 / Fragment: RRM DOMAIN, RESIDUES 28-121 Source method: isolated from a genetically manipulated source Source: (gene. exp.) CAENORHABDITIS ELEGANS (invertebrata) / Plasmid: PET-HIS1A / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): PLYSY / References: UniProt: H2L051 |
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#2: DNA chain | Mass: 2169.433 Da / Num. of mol.: 1 / Fragment: SUP-12 BINDING MOTIF / Source method: obtained synthetically Details: THE HEPTAMER SEQUENCE IS DERIVED FROM THE INTRON BETWEEN EXONS 4 AND 5B FROM THE C. ELEGANS EGL-15 GENE. Source: (synth.) CAENORHABDITIS ELEGANS (invertebrata) |
Sequence details | THE SAMPLE CONTAINS AN ADDITIONAL |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURE WAS DETERMINED BY USING MULTI- DIMENSIONAL NMR SPECTRSOCOPY WITH 15N-, 13C,15N-, OR 2H- LABELED SUP-12 IN COMPLEX WITH A NATURAL ABUNDANCE GGTGTGC DNA LIGAND, OR A LIGAND WITH ...Text: THE STRUCTURE WAS DETERMINED BY USING MULTI- DIMENSIONAL NMR SPECTRSOCOPY WITH 15N-, 13C,15N-, OR 2H- LABELED SUP-12 IN COMPLEX WITH A NATURAL ABUNDANCE GGTGTGC DNA LIGAND, OR A LIGAND WITH SITE-SPECIFIC 13C,15N- LABELING. |
-Sample preparation
Details |
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Sample conditions |
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-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: ARIA1.2 / Software ordinal: 1 | ||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST ENERGY AND NO VIOLATIONS Conformers calculated total number: 100 / Conformers submitted total number: 15 |