+Open data
-Basic information
Entry | Database: PDB / ID: 2nde | ||||||
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Title | Solution Structure of Mutant of BMAP-28(1-18) | ||||||
Components | Cathelicidin-5 | ||||||
Keywords | ANTIMICROBIAL PROTEIN / AMP | ||||||
Function / homology | Function and homology information lipopolysaccharide binding / antimicrobial humoral immune response mediated by antimicrobial peptide / defense response to Gram-negative bacterium / defense response to Gram-positive bacterium / innate immune response / extracellular space Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Agadi, N. / Kumar, A. | ||||||
Citation | Journal: To be Published Title: Structure, Function And Membrane Interaction Studies of Two Synthetic Peptides Using Solution And Solid State NMR Authors: Agadi, N. / Kumar, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2nde.cif.gz | 66.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2nde.ent.gz | 51.2 KB | Display | PDB format |
PDBx/mmJSON format | 2nde.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nd/2nde ftp://data.pdbj.org/pub/pdb/validation_reports/nd/2nde | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 1999.494 Da / Num. of mol.: 1 / Fragment: UNP residues 132-149 / Mutation: G1I/S5G/L11A/R12L/W14H / Source method: obtained synthetically / Source: (synth.) Bos taurus (cattle) / References: UniProt: P54229 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 20 mM sodium phosphate-1, 10 % [U-99% 2H] D2O-2, 90 % H2O-3, 50 mM [U-99% 2H] SDS-4, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 0 / pH: 6.6 / Pressure: ambient / Temperature: 298 |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 200 / Conformers submitted total number: 10 / Representative conformer: 1 |