+Open data
-Basic information
Entry | Database: PDB / ID: 1wc8 | ||||||
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Title | The crystal structure of mouse bet3p | ||||||
Components | TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT3 | ||||||
Keywords | TRANSPORT PROTEIN / VESICLE TRANSPORT / TETHERING FACTOR / TRAPP / GOLGI STACK / ENDOPLASMIC RETICULUM | ||||||
Function / homology | Function and homology information vesicle coating / vesicle tethering / RAB GEFs exchange GTP for GDP on RABs / COPII-mediated vesicle transport / TRAPPII protein complex / TRAPPIII protein complex / TRAPP complex / COPII vesicle coating / intra-Golgi vesicle-mediated transport / cis-Golgi network membrane ...vesicle coating / vesicle tethering / RAB GEFs exchange GTP for GDP on RABs / COPII-mediated vesicle transport / TRAPPII protein complex / TRAPPIII protein complex / TRAPP complex / COPII vesicle coating / intra-Golgi vesicle-mediated transport / cis-Golgi network membrane / endoplasmic reticulum to Golgi vesicle-mediated transport / Golgi membrane / Golgi apparatus / endoplasmic reticulum / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | MUS MUSCULUS (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / OTHER / Resolution: 1.9 Å | ||||||
Authors | Kim, Y.-G. / Sacher, M. / Oh, B.-H. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2005 Title: Crystal Structure of Bet3 Reveals a Novel Mechanism for Golgi Localization of Tethering Factor Trapp Authors: Kim, Y.-G. / Sohn, E.J. / Seo, J. / Lee, K.-J. / Lee, H.-S. / Hwang, I. / Whiteway, M. / Sacher, M. / Oh, B.-H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wc8.cif.gz | 41.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wc8.ent.gz | 33.1 KB | Display | PDB format |
PDBx/mmJSON format | 1wc8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wc/1wc8 ftp://data.pdbj.org/pub/pdb/validation_reports/wc/1wc8 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 20324.096 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) MUS MUSCULUS (house mouse) / Plasmid: PGEX 4T1 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: O55013 |
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#2: Chemical | ChemComp-MYR / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.06 Å3/Da / Density % sol: 40.26 % |
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Crystal grow | pH: 5.8 Details: 28% POLYETHYLENE GLYCOL 600, 5% POLYETHYLENE GLYCOL 1000, 10% GLYCEROL, AND 0.1 M MES (PH 5.8) |
-Data collection
Diffraction | Mean temperature: 193 K | ||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: PAL/PLS / Beamline: 6B / Wavelength: 0.9728, 0.97942, 0.97175 | ||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1.9→30 Å / Num. obs: 141514 / % possible obs: 97.7 % / Observed criterion σ(I): 1 / Redundancy: 10.2 % / Rmerge(I) obs: 0.04 |
-Processing
Software |
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Refinement | Method to determine structure: OTHER / Resolution: 1.9→10 Å / Cross valid method: THROUGHOUT / σ(F): 1
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.9→10 Å
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Refine LS restraints |
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Xplor file |
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