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- PDB-5li0: 70S ribosome from Staphylococcus aureus -

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Entry
Database: PDB / ID: 5li0
Title70S ribosome from Staphylococcus aureus
Components
  • (30S ribosomal protein ...) x 19
  • (50S ribosomal protein ...) x 28
  • 16S ribosomal RNA
  • 23S ribosomal RNA
  • 5S ribosomal RNA
KeywordsRIBOSOME / pathogenic ribosome / bL31 B-type / ribosome
Function / homologyL28p-like / Ribosomal protein L10e/L16 superfamily / Ribosomal protein L29/L35 superfamily / L21-like superfamily / Ribosomal L18e/L15P superfamily / Ribosomal protein L17 superfamily / Ribosomal protein L22/L17 superfamily / Ribosomal protein S3, C-terminal domain superfamily / Ribosomal protein S20 superfamily / Ribosomal protein L6, alpha-beta domain superfamily ...L28p-like / Ribosomal protein L10e/L16 superfamily / Ribosomal protein L29/L35 superfamily / L21-like superfamily / Ribosomal L18e/L15P superfamily / Ribosomal protein L17 superfamily / Ribosomal protein L22/L17 superfamily / Ribosomal protein S3, C-terminal domain superfamily / Ribosomal protein S20 superfamily / Ribosomal protein L6, alpha-beta domain superfamily / Ribosomal protein S7 domain superfamily / Ribosomal protein S10 domain superfamily / Ribosomal protein L14 superfamily / Ribosomal protein S18 superfamily / Ribosomal protein L13 superfamily / Ribosomal protein L30, ferredoxin-like fold domain superfamily / Ribosomal protein S11 superfamily / Ribosomal protein S6 superfamily / RNA-binding S4 domain superfamily / Ribosomal protein L25, C-terminal / Ribosomal protein L28/L24 superfamily / Ribosomal protein L35 superfamily / Ribosomal protein L33 superfamily / Ribosomal protein L19 superfamily / Ribosomal protein S4/S9 N-terminal domain / Ribosomal protein S12/S23 / Ribosomal protein S7p/S5e / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein S3, C-terminal domain / Ribosomal protein S19 / Ribosomal protein L22p/L17e / Ribosomal protein L14p/L23e / Ribosomal protein S8 superfamily / Ribosomal protein L36 superfamily / Ribosomal protein S14p/S29e / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L16, conserved site / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein S6, conserved site / Ribosomal protein L5, conserved site / Ribosomal protein L5, bacterial-type / Ribosomal protein S19 conserved site / Ribosomal protein L34, conserved site / Ribosomal protein L18e/L15P / Ribosomal protein L35 / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein L2, C-terminal / Ribosomal protein L2, conserved site / Ribosomal protein S4/S9 / Ribosomal protein L5 domain superfamily / Ribosomal protein S14, type Z / Ribosomal protein L20, C-terminal / Ribosomal protein S10 domain / Ribosomal protein L5, N-terminal / Ribosomal protein L5, C-terminal / Ribosomal protein L15 / Ribosomal protein L25 / Ribosomal protein L21-like / Ribosomal protein L31 type B / 30s ribosomal protein S13, C-terminal / Ribosomal protein L13, conserved site / Ribosomal protein L28/L24 / Ribosomal protein S16 domain superfamily / Ribosomal protein S7 domain / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S19, superfamily / Ribosomal protein L4 domain superfamily / Ribosomal protein L16p/L10e / Ribosomal protein L23 / Ribosomal protein L16 signature 2. / Ribosomal protein S19 signature. / Ribosomal protein S20 / Ribosomal L32p protein family / Ribosomal protein S5, C-terminal domain / Ribosomal Proteins L2, C-terminal domain / KH domain / Ribosomal protein TL5, C-terminal domain / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein L14 signature. / Ribosomal protein S7 signature. / Ribosomal protein S8 signature. / Ribosomal protein S11 signature. / Ribosomal protein S12 signature. / Ribosomal protein S17 signature. / Ribosomal protein S18 signature. / Ribosomal protein L5 signature. / S4 domain / Ribosomal protein S9 signature. / Ribosomal protein S10 signature. / Ribosomal protein S15 signature. / Ribosomal protein L22 signature. / Ribosomal protein L2 signature. / Ribosomal protein L3 signature. / Ribosomal protein L15 signature. / Ribosomal protein L6 signature 1. / Ribosomal protein S14 signature. / Ribosomal protein S3 signature. / Ribosomal protein L33 signature. / Ribosomal protein S5 signature. / Ribosomal protein L16 signature 1.
Function and homology information
Specimen sourceStaphylococcus aureus (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / 3.8 Å resolution
AuthorsKhusainov, I. / Vicens, Q. / Bochler, A. / Grosse, F. / Myasnikov, A. / Menetret, J.F. / Chicher, J. / Marzi, S. / Romby, P. / Yusupova, G. / Yusupov, M. / Hashem, Y.
CitationJournal: Nucleic Acids Res. / Year: 2016
Title: Structure of the 70S ribosome from human pathogen Staphylococcus aureus.
Authors: Iskander Khusainov / Quentin Vicens / Anthony Bochler / François Grosse / Alexander Myasnikov / Jean-François Ménétret / Johana Chicher / Stefano Marzi / Pascale Romby / Gulnara Yusupova / Marat Yusupov / Yaser Hashem
Abstract: Comparative structural studies of ribosomes from various organisms keep offering exciting insights on how species-specific or environment-related structural features of ribosomes may impact ...Comparative structural studies of ribosomes from various organisms keep offering exciting insights on how species-specific or environment-related structural features of ribosomes may impact translation specificity and its regulation. Although the importance of such features may be less obvious within more closely related organisms, their existence could account for vital yet species-specific mechanisms of translation regulation that would involve stalling, cell survival and antibiotic resistance. Here, we present the first full 70S ribosome structure from Staphylococcus aureus, a Gram-positive pathogenic bacterium, solved by cryo-electron microscopy. Comparative analysis with other known bacterial ribosomes pinpoints several unique features specific to S. aureus around a conserved core, at both the protein and the RNA levels. Our work provides the structural basis for the many studies aiming at understanding translation regulation in S. aureus and for designing drugs against this often multi-resistant pathogen.
Validation Report
SummaryFull reportAbout validation report
DateDeposition: Jul 13, 2016 / Release: Dec 28, 2016
RevisionDateData content typeGroupCategoryItemProviderType
1.0Dec 28, 2016Structure modelrepositoryInitial release
1.1Aug 30, 2017Structure modelData collection / Derived calculationsem_software / struct_conn_em_software.name

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Structure visualization

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Assembly

Deposited unit
a: 16S ribosomal RNA
b: 30S ribosomal protein S2
c: 30S ribosomal protein S3
d: 30S ribosomal protein S4
e: 30S ribosomal protein S5
f: 30S ribosomal protein S6
g: 30S ribosomal protein S7
h: 30S ribosomal protein S8
i: 30S ribosomal protein S9
j: 30S ribosomal protein S10
k: 30S ribosomal protein S11
l: 30S ribosomal protein S12
m: 30S ribosomal protein S13
n: 30S ribosomal protein S14 type Z
o: 30S ribosomal protein S15
p: 30S ribosomal protein S16
q: 30S ribosomal protein S17
r: 30S ribosomal protein S18
s: 30S ribosomal protein S19
t: 30S ribosomal protein S20
A: 23S ribosomal RNA
B: 5S ribosomal RNA
D: 50S ribosomal protein L2
E: 50S ribosomal protein L3
F: 50S ribosomal protein L4
G: 50S ribosomal protein L5
H: 50S ribosomal protein L6
M: 50S ribosomal protein L13
N: 50S ribosomal protein L14
O: 50S ribosomal protein L15
P: 50S ribosomal protein L16
Q: 50S ribosomal protein L17
R: 50S ribosomal protein L18
S: 50S ribosomal protein L19
T: 50S ribosomal protein L20
U: 50S ribosomal protein L21
V: 50S ribosomal protein L22
W: 50S ribosomal protein L23
X: 50S ribosomal protein L24
Y: 50S ribosomal protein L25
Z: 50S ribosomal protein L27
0: 50S ribosomal protein L28
1: 50S ribosomal protein L29
2: 50S ribosomal protein L30
3: 50S ribosomal protein L31 type B
4: 50S ribosomal protein L32
5: 50S ribosomal protein L33 2
6: 50S ribosomal protein L34
7: 50S ribosomal protein L35
8: 50S ribosomal protein L36
hetero molecules


Theoretical massNumber of molelcules
Total (without water)2,097,74091
Polyers2,096,77750
Non-polymers96341
Water2,918162
1


  • idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area (Å2)242610
ΔGint (kcal/M)-2240
Surface area (Å2)785920
MethodPISA

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Components

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RNA chain , 3 types, 3 molecules aAB

#1: RNA chain 16S ribosomal RNA /


Mass: 500464.406 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: GenBank: 87201381
#21: RNA chain 23S ribosomal RNA /


Mass: 945058.562 Da / Num. of mol.: 1
Source: (gene. exp.) Staphylococcus aureus (strain NCTC 8325) (bacteria)
Production host: Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: GenBank: 87201381
#22: RNA chain 5S ribosomal RNA /


Mass: 36692.801 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: GenBank: 87201381

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30S ribosomal protein ... , 19 types, 19 molecules bcdefghijklmnopqrst

#2: Protein/peptide 30S ribosomal protein S2 /


Mass: 29136.369 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FZ25
#3: Protein/peptide 30S ribosomal protein S3 /


Mass: 24143.867 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW12
#4: Protein/peptide 30S ribosomal protein S4 /


Mass: 22920.221 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FXK6
#5: Protein/peptide 30S ribosomal protein S5 /


Mass: 17639.314 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW23
#6: Protein/peptide 30S ribosomal protein S6 /


Mass: 11368.879 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2G113
#7: Protein/peptide 30S ribosomal protein S7 /


Mass: 17167.736 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: P48940
#8: Protein/peptide 30S ribosomal protein S8 /


Mass: 14723.118 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW20
#9: Protein/peptide 30S ribosomal protein S9 /


Mass: 14440.450 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW39
#10: Protein/peptide 30S ribosomal protein S10 /


Mass: 11600.520 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q931G5
#11: Protein/peptide 30S ribosomal protein S11 /


Mass: 12562.294 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW31
#12: Protein/peptide 30S ribosomal protein S12 /


Mass: 15131.598 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q5HID0
#13: Protein/peptide 30S ribosomal protein S13 /


Mass: 13487.543 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW30
#14: Protein/peptide 30S ribosomal protein S14 type Z / Ribosome


Mass: 7186.573 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW19
#15: Protein/peptide 30S ribosomal protein S15 /


Mass: 10503.135 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2G2Q1
#16: Protein/peptide 30S ribosomal protein S16 /


Mass: 10122.690 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FZ45
#17: Protein/peptide 30S ribosomal protein S17 /


Mass: 10065.691 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW15
#18: Protein/peptide 30S ribosomal protein S18 /


Mass: 8418.959 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2G111
#19: Protein/peptide 30S ribosomal protein S19 /


Mass: 9605.988 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW10
#20: Protein/peptide 30S ribosomal protein S20 /


Mass: 8708.018 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FXY6

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50S ribosomal protein ... , 28 types, 28 molecules DEFGHMNOPQRSTUVWXYZ012345678

#23: Protein/peptide 50S ribosomal protein L2 /


Mass: 29956.789 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: P60430
#24: Protein/peptide 50S ribosomal protein L3 /


Mass: 23499.877 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW06
#25: Protein/peptide 50S ribosomal protein L4 /


Mass: 21666.725 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW07
#26: Protein/peptide 50S ribosomal protein L5 /


Mass: 18729.721 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW18
#27: Protein/peptide 50S ribosomal protein L6 /


Mass: 18296.711 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW21
#28: Protein/peptide 50S ribosomal protein L13 /


Mass: 16359.427 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW38
#29: Protein/peptide 50S ribosomal protein L14 /


Mass: 13157.342 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW16
#30: Protein/peptide 50S ribosomal protein L15 /


Mass: 14193.304 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: P0A0F8
#31: Protein/peptide 50S ribosomal protein L16 /


Mass: 15943.755 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW13
#32: Protein/peptide 50S ribosomal protein L17 /


Mass: 13420.350 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW33
#33: Protein/peptide 50S ribosomal protein L18 /


Mass: 13124.093 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW22
#34: Protein/peptide 50S ribosomal protein L19 /


Mass: 12594.825 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FZ42
#35: Protein/peptide 50S ribosomal protein L20 /


Mass: 13459.919 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FXQ1
#36: Protein/peptide 50S ribosomal protein L21 /


Mass: 11354.081 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FXS8
#37: Protein/peptide 50S ribosomal protein L22 /


Mass: 12328.358 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW11
#38: Protein/peptide 50S ribosomal protein L23 /


Mass: 10380.100 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW08
#39: Protein/peptide 50S ribosomal protein L24 /


Mass: 10976.878 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW17
#40: Protein/peptide 50S ribosomal protein L25 / / General stress protein CTC


Mass: 10522.318 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2G0S0
#41: Protein/peptide 50S ribosomal protein L27 /


Mass: 8911.099 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FXT0
#42: Protein/peptide 50S ribosomal protein L28 /


Mass: 5312.262 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FZ60
#43: Protein/peptide 50S ribosomal protein L29 /


Mass: 7660.762 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW14
#44: Protein/peptide 50S ribosomal protein L30 /


Mass: 6305.308 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: P0A0G2
#45: Protein/peptide 50S ribosomal protein L31 type B / Ribosome


Mass: 9737.912 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FWD8
#46: Protein/peptide 50S ribosomal protein L32 /


Mass: 6397.427 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FZF1
#47: Protein/peptide 50S ribosomal protein L33 2 / Ribosome


Mass: 4711.371 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FY22
#48: Protein/peptide 50S ribosomal protein L34 /


Mass: 5323.446 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FUQ0
#49: Protein/peptide 50S ribosomal protein L35 /


Mass: 6985.402 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FXQ0
#50: Protein/peptide 50S ribosomal protein L36 /


Mass: 4318.422 Da / Num. of mol.: 1
Source: (natural) Staphylococcus aureus (strain NCTC 8325) (bacteria)
References: UniProt: Q2FW29

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Non-polymers , 4 types, 203 molecules

#51: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 31 / Formula: Mg / Magnesium
#52: Chemical
ChemComp-O / OXYGEN ATOM


Mass: 15.999 Da / Num. of mol.: 9 / Formula: O / Oxygen
#53: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 1 / Formula: Zn / Zinc
#54: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 162 / Formula: H2O / Water

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / Reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: 70S ribosome from Staphylococcus aureusRibosome / Type: RIBOSOME
Entity ID: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Buffer solutionDetails: 5 mM Hepes-KOH pH 7.5 50 mM KCl 10 mM NH4Cl 10 mM Mg(OAc)2 1 mM DTT
pH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: COPPER
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 kelvins / Details: blot force 5, blot waiting time 30 s

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyMicroscope model: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: DARK FIELD
Image recordingAverage exposure time: 1 sec. / Electron dose: 60 e/Å2 / Detector mode: COUNTING / Film or detector model: FEI FALCON II (4k x 4k) / Number of grids imaged: 1 / Number of real images: 3850
Image scansMovie frames/image: 17 / Used frames/image: 2-8

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Processing

SoftwareName: PHENIX / Version: dev_2341: / Classification: refinement
EM software
IDNameVersionCategory
1Xmipp3particle selection
2EPUimage acquisition
4CTFFIND4CTF correction
7VMD1.9.2model fitting
8NAMD2model fitting
10PHENIX1.10.1-2155model refinement
11PHENIXdev-2341model refinement
12Erraser1.10.1-2155model refinement
13RELION1.3initial Euler assignment
14RELION1.3final Euler assignment
15RELION1.3classification
16RELION1.33D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Particle selectionNumber of particles selected: 423497
3D reconstructionResolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 110000 / Algorithm: FOURIER SPACE / Number of class averages: 8 / Symmetry type: POINT
Atomic model buildingRef protocol: OTHER / Ref space: REAL
Refine LS restraints
Refine IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.011246899
ELECTRON MICROSCOPYf_angle_d1.183443889
ELECTRON MICROSCOPYf_dihedral_angle_d17.669107275
ELECTRON MICROSCOPYf_chiral_restr0.05529332
ELECTRON MICROSCOPYf_plane_restr0.00621135

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