根拠: isothermal titration calorimetry, The apo-form was obtained by complete refolding of protein-ligand complex. The addition of ligand (SAH or SAM) induced energy release observed in ITC ...根拠: isothermal titration calorimetry, The apo-form was obtained by complete refolding of protein-ligand complex. The addition of ligand (SAH or SAM) induced energy release observed in ITC experiment. It indicate that we are dealing with apo-from of WBSCR27 protein., native gel electrophoresis, Native gel electrophoresis showed that WBSCR27 in our samples is represented by a monomer., gel filtration, The gel filtration experiment indicated that WBSCR27 in our samples is represented by a monomer., NMR relaxation study, NMR relaxation data showed that WBSCR27 in our samples is represented by a monomer.
タイプ
名称
対称操作
数
identity operation
1_555
1
Buried area
0 Å2
ΔGint
0 kcal/mol
Surface area
16480 Å2
手法
PISA
NMR アンサンブル
データ
基準
コンフォーマー数 (登録 / 計算)
20 / 200
structures with the lowest energy
代表モデル
モデル #1
closest to the average
-
要素
#1: タンパク質
Methyltransferase-like27 / Williams-Beuren syndrome critical region protein 27 / Williams-Beuren syndrome critical region ...Williams-Beuren syndrome critical region protein 27 / Williams-Beuren syndrome critical region protein 27 isoform alpha / Williams-Beuren syndrome critical region protein 27 isoform beta