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- PDB-7lbm: Structure of the human Mediator-bound transcription pre-initiatio... -

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基本情報

登録情報
データベース: PDB / ID: 7lbm
タイトルStructure of the human Mediator-bound transcription pre-initiation complex
要素
  • (DNA-directed RNA polymerase II subunit ...ポリメラーゼ) x 8
  • (DNA-directed RNA polymerases I, II, and III subunit ...RNAポリメラーゼ) x 4
  • (General transcription factor IIF subunit ...) x 2
  • (General transcription factor IIH subunit ...) x 5
  • (Mediator of RNA polymerase II transcription subunit ...) x 23
  • (TFIIH basal transcription factor complex helicase ...) x 2
  • (Transcription initiation factor ...) x 4
  • (super core promoter ...) x 2
  • CDK-activating kinase assembly factor MAT1
  • Cyclin-H
  • Cyclin-dependent kinase 7
  • General transcription factor IIE subunit 1
  • Isoform 2 of Mediator of RNA polymerase II transcription subunit 16
  • TATA-box-binding protein
キーワードTRANSCRIPTION/DNA / pre-initiation complex / Mediator / initiation / complex / transcription (転写 (生物学)) / TRANSCRIPTION-DNA complex
機能・相同性
機能・相同性情報


positive regulation of mediator complex assembly / positive regulation of T cell extravasation / negative regulation of smooth muscle cell differentiation / LRR domain binding / microfibril binding / RNA Polymerase III Chain Elongation / MMXD complex / core TFIIH complex portion of holo TFIIH complex / RNA Polymerase III Transcription Termination / positive regulation of DNA helicase activity ...positive regulation of mediator complex assembly / positive regulation of T cell extravasation / negative regulation of smooth muscle cell differentiation / LRR domain binding / microfibril binding / RNA Polymerase III Chain Elongation / MMXD complex / core TFIIH complex portion of holo TFIIH complex / RNA Polymerase III Transcription Termination / positive regulation of DNA helicase activity / negative regulation of DNA helicase activity / Cytosolic iron-sulfur cluster assembly / nucleotide-excision repair, DNA duplex unwinding / central nervous system myelin formation / positive regulation of mitotic recombination / regulation of transcription by RNA polymerase I / core mediator complex / positive regulation of core promoter binding / meiotic sister chromatid cohesion / RPAP3/R2TP/prefoldin-like complex / RNA polymerase II core complex assembly / hair cell differentiation / RNA polymerase transcription factor SL1 complex / ventricular system development / transcription factor TFIIE complex / hair follicle maturation / cyclin-dependent protein kinase activating kinase holoenzyme complex / snRNA transcription by RNA polymerase II / phosphatase activator activity / nucleotide-excision repair factor 3 complex / nucleotide-excision repair, preincision complex assembly / 紫外線 / CAK-ERCC2 complex / RNA polymerase III general transcription initiation factor activity / transcription factor TFIIK complex / transcription open complex formation at RNA polymerase II promoter / embryonic cleavage / TFIIF-class transcription factor complex binding / transcriptional start site selection at RNA polymerase II promoter / mediator complex / nuclear retinoic acid receptor binding / transcription factor TFIIF complex / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / Cytosolic sensors of pathogen-associated DNA / RNA Polymerase III Transcription Initiation From Type 3 Promoter / 5'-3' DNA helicase activity / Generic Transcription Pathway / RNA Polymerase III Abortive And Retractive Initiation / adult heart development / transcription factor TFIIA complex / female germ cell nucleus / G protein-coupled receptor internalization / : / transcription factor TFIIH holo complex / transcription factor TFIIH core complex / male pronucleus / female pronucleus / cyclin-dependent protein serine/threonine kinase activator activity / Abortive elongation of HIV-1 transcript in the absence of Tat / nuclear vitamin D receptor binding / FGFR2 alternative splicing / RNA polymerase II general transcription initiation factor binding / positive regulation of chromatin binding / 卵母細胞 / [RNA-polymerase]-subunit kinase / limb development / nuclear thyroid hormone receptor binding / 3'-5' DNA helicase activity / MicroRNA (miRNA) biogenesis / 転写開始前複合体 / RNA Polymerase I Transcription Termination / regulation of mitotic cell cycle phase transition / cyclin-dependent protein serine/threonine kinase regulator activity / : / Viral Messenger RNA Synthesis / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / hematopoietic stem cell proliferation / Signaling by FGFR2 IIIa TM / cell division site / termination of RNA polymerase II transcription / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / RNA Pol II CTD phosphorylation and interaction with CE / cortical actin cytoskeleton / RNA polymerase II general transcription initiation factor activity / spinal cord development / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / transcription factor TFIID complex / mRNA Capping / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / mRNA Splicing - Minor Pathway / RNA polymerase II complex binding / PIWI-interacting RNA (piRNA) biogenesis / protein acetylation / HIV Transcription Initiation / RNA Polymerase II HIV Promoter Escape / Transcription of the HIV genome / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
類似検索 - 分子機能
: / Mediator complex, subunit Med25, PTOV domain / Mediator complex, subunit Med25, synapsin 1 / Mediator of RNA polymerase II transcription subunit 25, von Willebrand factor type A domain / Mediator complex subunit 25, PTOV domain superfamily / : / Mediator complex subunit 25 PTOV activation and synapsin 2 / Mediator complex subunit 25 synapsin 1 / Mediator complex subunit 25 von Willebrand factor type A / Mediator complex subunit 16, central helical bridge ...: / Mediator complex, subunit Med25, PTOV domain / Mediator complex, subunit Med25, synapsin 1 / Mediator of RNA polymerase II transcription subunit 25, von Willebrand factor type A domain / Mediator complex subunit 25, PTOV domain superfamily / : / Mediator complex subunit 25 PTOV activation and synapsin 2 / Mediator complex subunit 25 synapsin 1 / Mediator complex subunit 25 von Willebrand factor type A / Mediator complex subunit 16, central helical bridge / : / : / ARC105/Med15 mediator subunit central domain / ARC105/Med15 mediator subunit C-terminal domain / Mediator complex, subunit Med29, metazoa / Mediator complex, subunit Med24, / Mediator complex subunit 24 N-terminal / Mediator complex subunit 29 / Mediator of RNA polymerase II transcription subunit 15 / Mediator complex, subunit Med23 / ARC105/Med15 mediator subunit N-terminal KIX domain / Mediator complex subunit 23 / Mediator complex subunit 28 / Mediator complex, subunit Med6, metazoa/plant / Mediator complex, subunit Med19, metazoa / Mediator complex, subunit Med30, metazoa / Mediator complex, subunit Med28 / Mediator of RNA polymerase II transcription subunit 9, metazoan / Mediator of RNA pol II transcription subunit 19 / Mediator complex subunit 30 / : / : / Mediator complex subunit 16, C-terminal / Mediator complex, subunit Med27 / Mediator complex subunit 27 / Mediator complex, subunit Med16 / Mediator complex subunit 16, N-terminal / Mediator complex, subunit Med10 / Transcription factor subunit Med10 of Mediator complex / Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Mediator complex, subunit Med7 superfmaily / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Soluble quinoprotein glucose/sorbosone dehydrogenase / Mediator complex, subunit Med7/Med21-like / MED7 protein / Mediator complex, subunit Med20 / Mediator complex, subunit Med14 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Mediator complex subunit MED14 / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Med18 protein / Transcription factor TFIIE alpha subunit, C-terminal / C-terminal general transcription factor TFIIE alpha / CyclinH/Ccl1 / Cyclin-dependent kinase 7 / TFIIH subunit Tfb4/GTF2H3 / Transcription factor Tfb4 / TFIIH C1-like domain / Ssl1-like / TFIIH subunit Ssl1/p44 / Ssl1-like / TFIIH C1-like domain / TFIIH C1-like domain / TFIIH p62 subunit, N-terminal / TFIIH subunit Tfb1/GTF2H1 / TFIIH p62 subunit, N-terminal domain / BSD domain / BSD domain superfamily / BSD domain / BSD domain profile. / domain in transcription factors and synapse-associated proteins / Transcription factor TFIIE beta subunit, DNA-binding domain / Transcription initiation factor TFIIE, beta subunit / TFA2, Winged helix domain 2 / TFIIE beta subunit core domain / TFA2 Winged helix domain 2 / TFIIE beta central core DNA-binding domain profile. / RAD3/XPD family / Helicase XPB/Ssl2 / Helical and beta-bridge domain / ERCC3/RAD25/XPB helicase, C-terminal domain
類似検索 - ドメイン・相同性
鉄・硫黄クラスター / デオキシリボ核酸 / DNA (> 10) / DNA (> 100) / Mediator of RNA polymerase II transcription subunit 19 / DNA-directed RNA polymerase II subunit RPB4 / Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 24 / Mediator of RNA polymerase II transcription subunit 6 ...鉄・硫黄クラスター / デオキシリボ核酸 / DNA (> 10) / DNA (> 100) / Mediator of RNA polymerase II transcription subunit 19 / DNA-directed RNA polymerase II subunit RPB4 / Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 24 / Mediator of RNA polymerase II transcription subunit 6 / General transcription factor IIF subunit 2 / General transcription and DNA repair factor IIH helicase subunit XPD / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / General transcription and DNA repair factor IIH helicase subunit XPB / TATA-box-binding protein / DNA-directed RNA polymerase II subunit RPB1 / General transcription factor IIE subunit 1 / Transcription initiation factor IIE subunit beta / DNA-directed RNA polymerase II subunit RPB2 / General transcription factor IIH subunit 1 / General transcription factor IIF subunit 1 / DNA-directed RNA polymerase II subunit RPB9 / Cyclin-dependent kinase 7 / Cyclin-H / CDK-activating kinase assembly factor MAT1 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerase II subunit RPB11-a / Transcription initiation factor IIA subunit 1 / Transcription initiation factor IIA subunit 2 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerase II subunit RPB7 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / Transcription initiation factor IIB / Mediator of RNA polymerase II transcription subunit 21 / General transcription factor IIH subunit 2 / General transcription factor IIH subunit 3 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 27 / General transcription factor IIH subunit 5 / Mediator of RNA polymerase II transcription subunit 25 / General transcription factor IIH subunit 4 / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 30 / Mediator of RNA polymerase II transcription subunit 15 / Mediator of RNA polymerase II transcription subunit 10 / Mediator of RNA polymerase II transcription subunit 18 / Mediator of RNA polymerase II transcription subunit 28 / Mediator of RNA polymerase II transcription subunit 20 / Mediator of RNA polymerase II transcription subunit 4 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 29 / Mediator of RNA polymerase II transcription subunit 11 / Mediator of RNA polymerase II transcription subunit 23 / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 31
類似検索 - 構成要素
生物種Homo sapiens (ヒト)
手法電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.8 Å
データ登録者Abdella, R. / Talyzina, A. / He, Y.
資金援助 米国, 5件
組織認可番号
Howard Hughes Medical Institute (HHMI) 米国
American Cancer SocietyIRG-15-173-21 米国
National Institutes of Health/National Cancer Institute (NIH/NCI)P01-CA092584 米国
National Institutes of Health/National Cancer Institute (NIH/NCI)U54-CA193419 米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)T32-GM008382 米国
引用ジャーナル: Science / : 2021
タイトル: Structure of the human Mediator-bound transcription preinitiation complex.
著者: R Abdella / A Talyzina / S Chen / C J Inouye / R Tjian / Y He /
要旨: Eukaryotic transcription requires the assembly of a multisubunit preinitiation complex (PIC) composed of RNA polymerase II (Pol II) and the general transcription factors. The coactivator Mediator is ...Eukaryotic transcription requires the assembly of a multisubunit preinitiation complex (PIC) composed of RNA polymerase II (Pol II) and the general transcription factors. The coactivator Mediator is recruited by transcription factors, facilitates the assembly of the PIC, and stimulates phosphorylation of the Pol II C-terminal domain (CTD) by the TFIIH subunit CDK7. Here, we present the cryo-electron microscopy structure of the human Mediator-bound PIC at a resolution below 4 angstroms. Transcription factor binding sites within Mediator are primarily flexibly tethered to the tail module. CDK7 is stabilized by multiple contacts with Mediator. Two binding sites exist for the Pol II CTD, one between the head and middle modules of Mediator and the other in the active site of CDK7, providing structural evidence for Pol II CTD phosphorylation within the Mediator-bound PIC.
履歴
登録2021年1月8日登録サイト: RCSB / 処理サイト: RCSB
改定 1.02021年3月24日Provider: repository / タイプ: Initial release
改定 1.12021年4月14日Group: Database references / カテゴリ: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID

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構造の表示

ムービー
  • 登録構造単位
  • Jmolによる作画
  • ダウンロード
  • EMマップとの重ね合わせ
  • マップデータ: EMDB-23255
  • UCSF Chimeraによる作画
  • ダウンロード
ムービービューア
構造ビューア分子:
MolmilJmol/JSmol

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集合体

登録構造単位
0: Isoform 2 of Mediator of RNA polymerase II transcription subunit 16
1: Mediator of RNA polymerase II transcription subunit 23
2: Mediator of RNA polymerase II transcription subunit 24
3: Mediator of RNA polymerase II transcription subunit 25
A: DNA-directed RNA polymerase II subunit RPB1
B: DNA-directed RNA polymerase II subunit RPB2
C: DNA-directed RNA polymerase II subunit RPB3
D: DNA-directed RNA polymerase II subunit RPB4
E: DNA-directed RNA polymerase II subunit RPB5
F: DNA-directed RNA polymerases I, II, and III subunit RPABC2
G: DNA-directed RNA polymerase II subunit RPB7
H: DNA-directed RNA polymerases I, II, and III subunit RPABC3
I: DNA-directed RNA polymerase II subunit RPB9
J: DNA-directed RNA polymerases I, II, and III subunit RPABC5
K: DNA-directed RNA polymerase II subunit RPB11-a
L: DNA-directed RNA polymerases I, II, and III subunit RPABC4
M: Transcription initiation factor IIA subunit 1
N: Transcription initiation factor IIA subunit 2
O: Transcription initiation factor IIB
P: TATA-box-binding protein
Q: General transcription factor IIE subunit 1
R: Transcription initiation factor IIE subunit beta
S: General transcription factor IIF subunit 1
T: General transcription factor IIF subunit 2
U: super core promoter sense strand
V: super core promoter antisense strand
W: TFIIH basal transcription factor complex helicase XPB subunit
X: TFIIH basal transcription factor complex helicase XPD subunit
Y: General transcription factor IIH subunit 1
Z: General transcription factor IIH subunit 4, p52
a: General transcription factor IIH subunit 2
b: General transcription factor IIH subunit 3
c: General transcription factor IIH subunit 5
d: CDK-activating kinase assembly factor MAT1
e: Cyclin-dependent kinase 7
f: Cyclin-H
g: Mediator of RNA polymerase II transcription subunit 6
h: Mediator of RNA polymerase II transcription subunit 8
i: Mediator of RNA polymerase II transcription subunit 11
j: Mediator of RNA polymerase II transcription subunit 17
k: Mediator of RNA polymerase II transcription subunit 18
l: Mediator of RNA polymerase II transcription subunit 20
m: Mediator of RNA polymerase II transcription subunit 22
n: Mediator of RNA polymerase II transcription subunit 27
o: Mediator of RNA polymerase II transcription subunit 28
p: Mediator of RNA polymerase II transcription subunit 29
q: Mediator of RNA polymerase II transcription subunit 30
r: Mediator of RNA polymerase II transcription subunit 14
s: Mediator of RNA polymerase II transcription subunit 4
t: Mediator of RNA polymerase II transcription subunit 7
u: Mediator of RNA polymerase II transcription subunit 9
v: Mediator of RNA polymerase II transcription subunit 10
w: Mediator of RNA polymerase II transcription subunit 19
x: Mediator of RNA polymerase II transcription subunit 21
y: Mediator of RNA polymerase II transcription subunit 31
z: Mediator of RNA polymerase II transcription subunit 15
ヘテロ分子


分子量 (理論値)分子数
合計 (水以外)2,509,24475
ポリマ-2,507,79856
非ポリマー1,44719
0
1


  • 登録構造と同一
  • 登録者が定義した集合体
  • 根拠: microscopy
タイプ名称対称操作
identity operation1_5551
Buried area261170 Å2
ΔGint-1594 kcal/mol
Surface area699860 Å2

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要素

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タンパク質 , 6種, 6分子 0PQdef

#1: タンパク質 Isoform 2 of Mediator of RNA polymerase II transcription subunit 16 / Mediator complex subunit 16 / Thyroid hormone receptor-associated protein 5 / Thyroid hormone ...Mediator complex subunit 16 / Thyroid hormone receptor-associated protein 5 / Thyroid hormone receptor-associated protein complex 95 kDa component / Trap95 / Vitamin D3 receptor-interacting protein complex 92 kDa component / DRIP92


分子量: 93136.039 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q9Y2X0
#20: タンパク質 TATA-box-binding protein / TATA sequence-binding protein / TATA-binding factor / TATA-box factor / Transcription initiation ...TATA sequence-binding protein / TATA-binding factor / TATA-box factor / Transcription initiation factor TFIID TBP subunit


分子量: 37729.938 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: TBP, GTF2D1, TF2D, TFIID / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P20226
#21: タンパク質 General transcription factor IIE subunit 1 / General transcription factor IIE 56 kDa subunit / Transcription initiation factor IIE subunit alpha ...General transcription factor IIE 56 kDa subunit / Transcription initiation factor IIE subunit alpha / TFIIE-alpha


分子量: 49516.094 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: GTF2E1, TF2E1 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P29083
#34: タンパク質 CDK-activating kinase assembly factor MAT1 / CDK7/cyclin-H assembly factor / Cyclin-G1-interacting protein / Menage a trois / RING finger ...CDK7/cyclin-H assembly factor / Cyclin-G1-interacting protein / Menage a trois / RING finger protein 66 / RING finger protein MAT1 / p35 / p36


分子量: 35873.965 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 参照: UniProt: P51948
#35: タンパク質 Cyclin-dependent kinase 7 / / 39 kDa protein kinase / p39 Mo15 / CDK-activating kinase 1 / Cell division protein kinase 7 / ...39 kDa protein kinase / p39 Mo15 / CDK-activating kinase 1 / Cell division protein kinase 7 / Serine/threonine-protein kinase 1 / TFIIH basal transcription factor complex kinase subunit


分子量: 39090.344 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA
参照: UniProt: P50613, サイクリン依存性キナーゼ, [RNA-polymerase]-subunit kinase
#36: タンパク質 Cyclin-H / MO15-associated protein / p34 / p37


分子量: 37695.473 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 参照: UniProt: P51946

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Mediator of RNA polymerase II transcription subunit ... , 23種, 23分子 123ghijklmnopqrstuvwxyz

#2: タンパク質 Mediator of RNA polymerase II transcription subunit 23 / Activator-recruited cofactor 130 kDa component / ARC130 / Cofactor required for Sp1 transcriptional ...Activator-recruited cofactor 130 kDa component / ARC130 / Cofactor required for Sp1 transcriptional activation subunit 3 / CRSP complex subunit 3 / Mediator complex subunit 23 / Protein sur-2 homolog / hSur-2 / Transcriptional coactivator CRSP130 / Vitamin D3 receptor-interacting protein complex 130 kDa component / DRIP130


分子量: 156652.156 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 参照: UniProt: Q9ULK4
#3: タンパク質 Mediator of RNA polymerase II transcription subunit 24 / Activator-recruited cofactor 100 kDa component / ARC100 / Cofactor required for Sp1 transcriptional ...Activator-recruited cofactor 100 kDa component / ARC100 / Cofactor required for Sp1 transcriptional activation subunit 4 / CRSP complex subunit 4 / Mediator complex subunit 24 / Thyroid hormone receptor-associated protein 4 / Thyroid hormone receptor-associated protein complex 100 kDa component / Trap100 / hTRAP100 / Vitamin D3 receptor-interacting protein complex 100 kDa component / DRIP100


分子量: 110430.164 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: O75448
#4: タンパク質 Mediator of RNA polymerase II transcription subunit 25 / Activator interaction domain-containing protein 1 / Activator-recruited cofactor 92 kDa component / ...Activator interaction domain-containing protein 1 / Activator-recruited cofactor 92 kDa component / ARC92 / Mediator complex subunit 25 / p78


分子量: 78230.344 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q71SY5
#37: タンパク質 Mediator of RNA polymerase II transcription subunit 6 / Activator-recruited cofactor 33 kDa component / ARC33 / Mediator complex subunit 6 / hMed6 / Renal ...Activator-recruited cofactor 33 kDa component / ARC33 / Mediator complex subunit 6 / hMed6 / Renal carcinoma antigen NY-REN-28


分子量: 28464.627 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: O75586
#38: タンパク質 Mediator of RNA polymerase II transcription subunit 8 / Activator-recruited cofactor 32 kDa component / ARC32 / Mediator complex subunit 8


分子量: 29112.906 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q96G25
#39: タンパク質 Mediator of RNA polymerase II transcription subunit 11 / Mediator complex subunit 11


分子量: 13145.829 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q9P086
#40: タンパク質 Mediator of RNA polymerase II transcription subunit 17 / Activator-recruited cofactor 77 kDa component / ARC77 / Cofactor required for Sp1 transcriptional ...Activator-recruited cofactor 77 kDa component / ARC77 / Cofactor required for Sp1 transcriptional activation subunit 6 / CRSP complex subunit 6 / Mediator complex subunit 17 / Thyroid hormone receptor-associated protein complex 80 kDa component / Trap80 / Transcriptional coactivator CRSP77 / Vitamin D3 receptor-interacting protein complex 80 kDa component / DRIP80


分子量: 72984.047 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q9NVC6
#41: タンパク質 Mediator of RNA polymerase II transcription subunit 18 / Mediator complex subunit 18 / p28b


分子量: 23693.730 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q9BUE0
#42: タンパク質 Mediator of RNA polymerase II transcription subunit 20 / Mediator complex subunit 20 / TRF-proximal protein homolog / hTRFP


分子量: 23243.762 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q9H944
#43: タンパク質 Mediator of RNA polymerase II transcription subunit 22 / Mediator complex subunit 22 / Surfeit locus protein 5 / Surf-5


分子量: 22243.713 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q15528
#44: タンパク質 Mediator of RNA polymerase II transcription subunit 27 / Cofactor required for Sp1 transcriptional activation subunit 8 / CRSP complex subunit 8 / Mediator ...Cofactor required for Sp1 transcriptional activation subunit 8 / CRSP complex subunit 8 / Mediator complex subunit 27 / P37 TRAP/SMCC/PC2 subunit / Transcriptional coactivator CRSP34


分子量: 35478.742 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q6P2C8
#45: タンパク質 Mediator of RNA polymerase II transcription subunit 28 / Endothelial-derived protein 1 / Mediator complex subunit 28 / Merlin and Grb2-interacting ...Endothelial-derived protein 1 / Mediator complex subunit 28 / Merlin and Grb2-interacting cytoskeletal protein / Magicin / Tumor angiogenesis marker EG-1


分子量: 19540.107 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q9H204
#46: タンパク質 Mediator of RNA polymerase II transcription subunit 29 / Intersex-like protein / Mediator complex subunit 29


分子量: 21089.801 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q9NX70
#47: タンパク質 Mediator of RNA polymerase II transcription subunit 30 / Mediator complex subunit 30 / TRAP/Mediator complex component TRAP25 / Thyroid hormone receptor- ...Mediator complex subunit 30 / TRAP/Mediator complex component TRAP25 / Thyroid hormone receptor-associated protein 6 / Thyroid hormone receptor-associated protein complex 25 kDa component / Trap25


分子量: 20301.229 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q96HR3
#48: タンパク質 Mediator of RNA polymerase II transcription subunit 14 / Activator-recruited cofactor 150 kDa component / ARC150 / Cofactor required for Sp1 transcriptional ...Activator-recruited cofactor 150 kDa component / ARC150 / Cofactor required for Sp1 transcriptional activation subunit 2 / CRSP complex subunit 2 / Mediator complex subunit 14 / RGR1 homolog / hRGR1 / Thyroid hormone receptor-associated protein complex 170 kDa component / Trap170 / Transcriptional coactivator CRSP150 / Vitamin D3 receptor-interacting protein complex 150 kDa component / DRIP150


分子量: 160787.594 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: O60244
#49: タンパク質 Mediator of RNA polymerase II transcription subunit 4 / Activator-recruited cofactor 36 kDa component / ARC36 / Mediator complex subunit 4 / TRAP/SMCC/PC2 ...Activator-recruited cofactor 36 kDa component / ARC36 / Mediator complex subunit 4 / TRAP/SMCC/PC2 subunit p36 subunit / Vitamin D3 receptor-interacting protein complex 36 kDa component / DRIP36


分子量: 29781.424 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q9NPJ6
#50: タンパク質 Mediator of RNA polymerase II transcription subunit 7 / hMED7 / Activator-recruited cofactor 34 kDa component / ARC34 / Cofactor required for Sp1 ...hMED7 / Activator-recruited cofactor 34 kDa component / ARC34 / Cofactor required for Sp1 transcriptional activation subunit 9 / CRSP complex subunit 9 / Mediator complex subunit 7 / RNA polymerase transcriptional regulation mediator subunit 7 homolog / Transcriptional coactivator CRSP33


分子量: 27285.281 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 参照: UniProt: O43513
#51: タンパク質 Mediator of RNA polymerase II transcription subunit 9 / Mediator complex subunit 9


分子量: 16422.750 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q9NWA0
#52: タンパク質 Mediator of RNA polymerase II transcription subunit 10 / Mediator complex subunit 10 / Transformation-related gene 17 protein / TRG-17 / Transformation- ...Mediator complex subunit 10 / Transformation-related gene 17 protein / TRG-17 / Transformation-related gene 20 protein / TRG-20


分子量: 15710.969 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q9BTT4
#53: タンパク質 Mediator of RNA polymerase II transcription subunit 19 / Lung cancer metastasis-related protein 1 / Mediator complex subunit 19


分子量: 26321.146 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: A0JLT2
#54: タンパク質 Mediator of RNA polymerase II transcription subunit 21 / Mediator complex subunit 21 / RNA polymerase II holoenzyme component SRB7 / RNAPII complex ...Mediator complex subunit 21 / RNA polymerase II holoenzyme component SRB7 / RNAPII complex component SRB7 / hSrb7


分子量: 15575.234 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 参照: UniProt: Q13503
#55: タンパク質 Mediator of RNA polymerase II transcription subunit 31 / Mediator complex subunit 31 / Mediator complex subunit SOH1 / hSOH1


分子量: 15826.116 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q9Y3C7
#56: タンパク質 Mediator of RNA polymerase II transcription subunit 15 / Activator-recruited cofactor 105 kDa component / ARC105 / CTG repeat protein 7a / Mediator complex ...Activator-recruited cofactor 105 kDa component / ARC105 / CTG repeat protein 7a / Mediator complex subunit 15 / Positive cofactor 2 glutamine/Q-rich-associated protein / PC2 glutamine/Q-rich-associated protein / TPA-inducible gene 1 protein / TIG-1 / Trinucleotide repeat-containing gene 7 protein


分子量: 86830.102 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q96RN5

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DNA-directed RNA polymerase II subunit ... , 8種, 8分子 ABCDEGIK

#5: タンパク質 DNA-directed RNA polymerase II subunit RPB1 / ポリメラーゼ / RNA polymerase II subunit B1 / DNA-directed RNA polymerase II subunit A / DNA-directed RNA ...RNA polymerase II subunit B1 / DNA-directed RNA polymerase II subunit A / DNA-directed RNA polymerase III largest subunit / RNA-directed RNA polymerase II subunit RPB1


分子量: 217420.047 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: P24928, ポリメラーゼ
#6: タンパク質 DNA-directed RNA polymerase II subunit RPB2 / ポリメラーゼ / DNA-directed RNA polymerase II 140 kDa polypeptide / DNA-directed RNA polymerase II subunit B / RNA ...DNA-directed RNA polymerase II 140 kDa polypeptide / DNA-directed RNA polymerase II subunit B / RNA polymerase II subunit 2 / RNA polymerase II subunit B2


分子量: 134071.453 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: P30876, ポリメラーゼ
#7: タンパク質 DNA-directed RNA polymerase II subunit RPB3 / ポリメラーゼ / RNA polymerase II subunit B3 / DNA-directed RNA polymerase II 33 kDa polypeptide / RPB33 / DNA- ...RNA polymerase II subunit B3 / DNA-directed RNA polymerase II 33 kDa polypeptide / RPB33 / DNA-directed RNA polymerase II subunit C / RPB31


分子量: 31478.148 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: P19387
#8: タンパク質 DNA-directed RNA polymerase II subunit RPB4 / ポリメラーゼ / RNA polymerase II subunit B4 / DNA-directed RNA polymerase II subunit D / RNA polymerase II 16 kDa ...RNA polymerase II subunit B4 / DNA-directed RNA polymerase II subunit D / RNA polymerase II 16 kDa subunit / RPB16


分子量: 16331.255 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: O15514
#9: タンパク質 DNA-directed RNA polymerase II subunit RPB5 / ポリメラーゼ / RNA polymerases I / II / and III subunit ABC1 / DNA-directed RNA DNA-directed RNA polymerases I / ...RNA polymerases I / II / and III subunit ABC1 / DNA-directed RNA DNA-directed RNA polymerases I / II / and III subunit RPABC1 / polymerase II 23 kDa polypeptide / DNA-directed RNA polymerase II subunit E / XAP4


分子量: 24584.223 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: P19388
#11: タンパク質 DNA-directed RNA polymerase II subunit RPB7 / ポリメラーゼ / RNA polymerase II subunit B7 / DNA-directed RNA polymerase II subunit G / RNA polymerase II 19 kDa ...RNA polymerase II subunit B7 / DNA-directed RNA polymerase II subunit G / RNA polymerase II 19 kDa subunit / RPB19


分子量: 19314.283 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: P62487
#13: タンパク質 DNA-directed RNA polymerase II subunit RPB9 / ポリメラーゼ / RNA polymerase II subunit B9 / DNA-directed RNA polymerase II subunit I / RNA polymerase II 14.5 ...RNA polymerase II subunit B9 / DNA-directed RNA polymerase II subunit I / RNA polymerase II 14.5 kDa subunit / RPB14.5


分子量: 14541.221 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: P36954
#15: タンパク質 DNA-directed RNA polymerase II subunit RPB11-a / ポリメラーゼ / RPB11a / DNA-directed RNA polymerase II subunit J-1 / RNA polymerase II 13.3 kDa subunit


分子量: 13310.284 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: P52435

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DNA-directed RNA polymerases I, II, and III subunit ... , 4種, 4分子 FHJL

#10: タンパク質 DNA-directed RNA polymerases I, II, and III subunit RPABC2 / RNAポリメラーゼ / RNA polymerases I / II / and III subunit ABC2 / DNA-directed RNA polymerase II subunit F / DNA- ...RNA polymerases I / II / and III subunit ABC2 / DNA-directed RNA polymerase II subunit F / DNA-directed RNA polymerases I / and III 14.4 kDa polypeptide / RPABC14.4 / RPB14.4 / RPB6 homolog / RPC15


分子量: 14491.026 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: P61218
#12: タンパク質 DNA-directed RNA polymerases I, II, and III subunit RPABC3 / RNAポリメラーゼ / RNA polymerases I / II / and III subunit ABC3 / DNA-directed RNA polymerase II subunit H / DNA- ...RNA polymerases I / II / and III subunit ABC3 / DNA-directed RNA polymerase II subunit H / DNA-directed RNA polymerases I / and III 17.1 kDa polypeptide / RPB17 / RPB8 homolog / hRPB8


分子量: 17162.273 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: P52434
#14: タンパク質 DNA-directed RNA polymerases I, II, and III subunit RPABC5 / RNAポリメラーゼ / RNA polymerases I / II / and III subunit ABC5 / DNA-directed RNA polymerase III subunit L / RNA ...RNA polymerases I / II / and III subunit ABC5 / DNA-directed RNA polymerase III subunit L / RNA polymerase II 7.6 kDa subunit / RPB7.6 / RPB10 homolog


分子量: 7655.123 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: P62875
#16: タンパク質 DNA-directed RNA polymerases I, II, and III subunit RPABC4 / RNAポリメラーゼ / RNA polymerases I / II / and III subunit ABC4 / ABC10-alpha / DNA-directed RNA polymerase II ...RNA polymerases I / II / and III subunit ABC4 / ABC10-alpha / DNA-directed RNA polymerase II subunit K / RNA polymerase II 7.0 kDa subunit / RPB7.0 / RPB10alpha


分子量: 7018.244 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: P53803

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Transcription initiation factor ... , 4種, 4分子 MNOR

#17: タンパク質 Transcription initiation factor IIA subunit 1 / General transcription factor IIA subunit 1 / TFIIAL / Transcription initiation factor TFIIA 42 kDa ...General transcription factor IIA subunit 1 / TFIIAL / Transcription initiation factor TFIIA 42 kDa subunit / TFIIA-42


分子量: 41544.551 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: GTF2A1, TF2A1 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P52655
#18: タンパク質 Transcription initiation factor IIA subunit 2 / General transcription factor IIA subunit 2 / TFIIA p12 subunit / TFIIAS / Transcription initiation ...General transcription factor IIA subunit 2 / TFIIA p12 subunit / TFIIAS / Transcription initiation factor IIA gamma chain / TFIIA-gamma


分子量: 12469.091 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: GTF2A2, TF2A2 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P52657
#19: タンパク質 Transcription initiation factor IIB / General transcription factor TFIIB / S300-II


分子量: 34877.949 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: GTF2B, TF2B, TFIIB / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: Q00403
#22: タンパク質 Transcription initiation factor IIE subunit beta / TFIIE-beta / General transcription factor IIE subunit 2


分子量: 33106.824 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: GTF2E2, TF2E2 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P29084

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General transcription factor IIF subunit ... , 2種, 2分子 ST

#23: タンパク質 General transcription factor IIF subunit 1 / General transcription factor IIF 74 kDa subunit / Transcription initiation factor IIF subunit alpha ...General transcription factor IIF 74 kDa subunit / Transcription initiation factor IIF subunit alpha / TFIIF-alpha / Transcription initiation factor RAP74


分子量: 58343.578 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: GTF2F1, RAP74 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P35269
#24: タンパク質 General transcription factor IIF subunit 2 / ATP-dependent helicase GTF2F2 / General transcription factor IIF 30 kDa subunit / Transcription ...ATP-dependent helicase GTF2F2 / General transcription factor IIF 30 kDa subunit / Transcription initiation factor IIF subunit beta / TFIIF-beta / Transcription initiation factor RAP30


分子量: 28427.309 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: GTF2F2, RAP30 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P13984, ヘリカーゼ

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Super core promoter ... , 2種, 2分子 UV

#25: DNA鎖 super core promoter sense strand


分子量: 31002.770 Da / 分子数: 1 / 由来タイプ: 合成 / 由来: (合成) Homo sapiens (ヒト)
#26: DNA鎖 super core promoter antisense strand


分子量: 30708.537 Da / 分子数: 1 / 由来タイプ: 合成 / 由来: (合成) Homo sapiens (ヒト)

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TFIIH basal transcription factor complex helicase ... , 2種, 2分子 WX

#27: タンパク質 TFIIH basal transcription factor complex helicase XPB subunit / Basic transcription factor 2 89 kDa subunit / BTF2 p89 / DNA excision repair protein ERCC-3 / DNA ...Basic transcription factor 2 89 kDa subunit / BTF2 p89 / DNA excision repair protein ERCC-3 / DNA repair protein complementing XP-B cells / TFIIH basal transcription factor complex 89 kDa subunit / TFIIH p89 / Xeroderma pigmentosum group B-complementing protein


分子量: 89404.734 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: P19447, ヘリカーゼ
#28: タンパク質 TFIIH basal transcription factor complex helicase XPD subunit / Basic transcription factor 2 80 kDa subunit / BTF2 p80 / CXPD / DNA excision repair protein ERCC-2 ...Basic transcription factor 2 80 kDa subunit / BTF2 p80 / CXPD / DNA excision repair protein ERCC-2 / DNA repair protein complementing XP-D cells / TFIIH basal transcription factor complex 80 kDa subunit / TFIIH p80 / Xeroderma pigmentosum group D-complementing protein


分子量: 87021.078 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: P18074, ヘリカーゼ

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General transcription factor IIH subunit ... , 5種, 5分子 YZabc

#29: タンパク質 General transcription factor IIH subunit 1 / Basic transcription factor 2 62 kDa subunit / BTF2 p62 / General transcription factor IIH ...Basic transcription factor 2 62 kDa subunit / BTF2 p62 / General transcription factor IIH polypeptide 1 / TFIIH basal transcription factor complex p62 subunit


分子量: 62116.492 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: P32780
#30: タンパク質 General transcription factor IIH subunit 4, p52 / Basic transcription factor 2 52 kDa subunit / BTF2 p52 / General transcription factor IIH ...Basic transcription factor 2 52 kDa subunit / BTF2 p52 / General transcription factor IIH polypeptide 4 / TFIIH basal transcription factor complex p52 subunit


分子量: 52245.156 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q92759
#31: タンパク質 General transcription factor IIH subunit 2 / Basic transcription factor 2 44 kDa subunit / BTF2 p44 / General transcription factor IIH ...Basic transcription factor 2 44 kDa subunit / BTF2 p44 / General transcription factor IIH polypeptide 2 / TFIIH basal transcription factor complex p44 subunit


分子量: 44481.996 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q13888
#32: タンパク質 General transcription factor IIH subunit 3 / p34 / Basic transcription factor 2 44 kDa subunit / BTF2 p44 / General transcription factor IIH ...p34 / Basic transcription factor 2 44 kDa subunit / BTF2 p44 / General transcription factor IIH polypeptide 2 / TFIIH basal transcription factor complex p44 subunit


分子量: 34416.008 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q13889
#33: タンパク質 General transcription factor IIH subunit 5 / General transcription factor IIH polypeptide 5 / TFB5 ortholog / TFIIH basal transcription factor ...General transcription factor IIH polypeptide 5 / TFB5 ortholog / TFIIH basal transcription factor complex TTD-A subunit


分子量: 8060.362 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 細胞株: HELA / 参照: UniProt: Q6ZYL4

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非ポリマー , 3種, 19分子

#57: 化合物 ChemComp-MG / MAGNESIUM ION / マグネシウムジカチオン


分子量: 24.305 Da / 分子数: 2 / 由来タイプ: 合成 / : Mg
#58: 化合物
ChemComp-ZN / ZINC ION


分子量: 65.409 Da / 分子数: 16 / 由来タイプ: 合成 / : Zn
#59: 化合物 ChemComp-SF4 / IRON/SULFUR CLUSTER / 鉄・硫黄クラスター


分子量: 351.640 Da / 分子数: 1 / 由来タイプ: 合成 / : Fe4S4

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詳細

研究の焦点であるリガンドがあるかN

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実験情報

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実験

実験手法: 電子顕微鏡法
EM実験試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法

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試料調製

構成要素
ID名称タイプEntity IDParent-ID由来
1Mediator-bound pre-initiation complexCOMPLEX#1-#560MULTIPLE SOURCES
2RNA Polymerase IIRNAポリメラーゼIICOMPLEX#5-#161NATURAL
3MediatorCOMPLEX#1-#4, #37-#561NATURAL
4TFIIHCOMPLEX#27-#331NATURAL
5General transcription factors基本転写因子COMPLEX#17-#241RECOMBINANT
6promoter DNACOMPLEX#25-#261SYNTHETIC
7cyclin-H and associated factorsCOMPLEX#34-#361NATURAL
分子量
IDEntity assembly-ID (°)実験値
112.7 MDaNO
220.52 MDaNO
331.4 MDaNO
440.5 MDaNO
550.3 MDaNO
66NO
77
由来(天然)
IDEntity assembly-ID生物種Ncbi tax-ID
12Homo sapiens (ヒト)9606
23Homo sapiens (ヒト)9606
34Homo sapiens (ヒト)9606
45Homo sapiens (ヒト)9606
56Homo sapiens (ヒト)9606
67Homo sapiens (ヒト)9606
由来(組換発現)生物種: Escherichia coli (大腸菌)
緩衝液pH: 7.9
試料包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES
急速凍結凍結剤: ETHANE

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電子顕微鏡撮影

実験機器
モデル: Titan Krios / 画像提供: FEI Company
顕微鏡モデル: FEI TITAN KRIOS
電子銃電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM
電子レンズモード: BRIGHT FIELDBright-field microscopy
撮影電子線照射量: 31.5 e/Å2
フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k)

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解析

CTF補正タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION
3次元再構成解像度: 4.8 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 156383 / 対称性のタイプ: POINT

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万見について

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お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月:コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか? / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例:em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る