+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1xw7 | ||||||
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タイトル | Diabetes-Associated Mutations in Human Insulin: Crystal Structure and Photo-Cross-Linking Studies of A-Chain Variant Insulin Wakayama | ||||||
要素 | (Insulinインスリン) x 2 | ||||||
キーワード | HORMONE/GROWTH FACTOR / Leu-A30insulin / protein unfolding / insulin receptor (インスリン受容体) / HORMONE-GROWTH FACTOR COMPLEX | ||||||
機能・相同性 | 機能・相同性情報 negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / Signaling by Insulin receptor / IRS activation / nitric oxide-cGMP-mediated signaling / negative regulation of fatty acid metabolic process / Insulin processing / negative regulation of feeding behavior / regulation of protein secretion ...negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / Signaling by Insulin receptor / IRS activation / nitric oxide-cGMP-mediated signaling / negative regulation of fatty acid metabolic process / Insulin processing / negative regulation of feeding behavior / regulation of protein secretion / positive regulation of peptide hormone secretion / Regulation of gene expression in beta cells / positive regulation of respiratory burst / positive regulation of dendritic spine maintenance / alpha-beta T cell activation / negative regulation of acute inflammatory response / negative regulation of respiratory burst involved in inflammatory response / negative regulation of protein secretion / fatty acid homeostasis / Synthesis, secretion, and deacylation of Ghrelin / positive regulation of glycogen biosynthetic process / positive regulation of lipid biosynthetic process / Signal attenuation / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / negative regulation of gluconeogenesis / positive regulation of nitric oxide mediated signal transduction / regulation of protein localization to plasma membrane / COPI-mediated anterograde transport / negative regulation of lipid catabolic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / positive regulation of insulin receptor signaling pathway / negative regulation of reactive oxygen species biosynthetic process / 小胞 / positive regulation of protein autophosphorylation / Insulin receptor recycling / insulin-like growth factor receptor binding / NPAS4 regulates expression of target genes / positive regulation of protein metabolic process / neuron projection maintenance / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of brown fat cell differentiation / activation of protein kinase B activity / positive regulation of glycolytic process / Insulin receptor signalling cascade / positive regulation of mitotic nuclear division / Regulation of insulin secretion / positive regulation of nitric-oxide synthase activity / positive regulation of long-term synaptic potentiation / endosome lumen / positive regulation of cytokine production / acute-phase response / positive regulation of protein secretion / regulation of transmembrane transporter activity / positive regulation of cell differentiation / positive regulation of glucose import / negative regulation of proteolysis / regulation of synaptic plasticity / wound healing / insulin receptor binding / negative regulation of protein catabolic process / positive regulation of neuron projection development / hormone activity / 認識 / Golgi lumen / vasodilation / positive regulation of protein localization to nucleus / glucose metabolic process / regulation of protein localization / glucose homeostasis / cell-cell signaling / insulin receptor signaling pathway / positive regulation of NF-kappaB transcription factor activity / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of cell growth / secretory granule lumen / protease binding / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of cell migration / G protein-coupled receptor signaling pathway / Amyloid fiber formation / 小胞体 / ゴルジ体 / negative regulation of gene expression / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of DNA-templated transcription / extracellular space / extracellular region / identical protein binding 類似検索 - 分子機能 | ||||||
手法 | X線回折 / 分子置換 / 解像度: 2.3 Å | ||||||
データ登録者 | Wan, Z.L. / Huang, K. / Xu, B. / Chu, Y.C. / Hu, S.Q. / Katsoyannis, P.G. / Weiss, M.A. | ||||||
引用 | ジャーナル: Biochemistry / 年: 2005 タイトル: Diabetes-associated mutations in human insulin: crystal structure and photo-cross-linking studies of a-chain variant insulin wakayama 著者: Wan, Z.L. / Huang, K. / Xu, B. / Hu, S.Q. / Wang, S. / Chu, Y.C. / Katsoyannis, P.G. / Weiss, M.A. #1: ジャーナル: Acta Crystallogr.,Sect.D / 年: 2003 タイトル: Crystallographic titration of cubic insulin crystals: pH affects GluB13 switching and sulfate binding 著者: Diao, J. #2: ジャーナル: Biophys.J. / 年: 1998 タイトル: Structure of cubic insulin crystals in glucose solutions 著者: Yu, B. / Caspar, D.L. #3: ジャーナル: Acta Crystallogr.,Sect.B / 年: 1991 タイトル: Structure of the pig insulin dimer in the cubic crystal 著者: Badger, J. / Harris, M.R. / Reynolds, C.D. / Evans, A.C. / Dodson, E.J. / Dodson, G.G. / North, A.C. #4: ジャーナル: Proc.Natl.Acad.Sci.USA / 年: 1991 タイトル: Water structure in cubic insulin crystals 著者: Badager, J. / Caspar, D.L. #5: ジャーナル: J.Mol.Biol. / 年: 1978 タイトル: Zinc-free cubic pig insulin: crystallization and structure determination 著者: Dodson, E.J. / Dodson, G.G. / Lewitova, A. / Sabesan, M. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1xw7.cif.gz | 36.4 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1xw7.ent.gz | 24.9 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1xw7.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/xw/1xw7 ftp://data.pdbj.org/pub/pdb/validation_reports/xw/1xw7 | HTTPS FTP |
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-関連構造データ
関連構造データ | 1zegS S: 精密化の開始モデル |
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類似構造データ |
-リンク
-集合体
登録構造単位 |
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単位格子 |
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Components on special symmetry positions |
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詳細 | The biological assembly is a hexamer generated from the dimer in the asymmetric unit by the operations |
-要素
-タンパク質・ペプチド , 2種, 4分子 ACBD
#1: タンパク質・ペプチド | 分子量: 2397.725 Da / 分子数: 2 / 変異: V3L / 由来タイプ: 合成 詳細: The peptide was chemically synthesized. The sequence of the peptide is naturally found in Homo sapiens (human). 参照: UniProt: P01308 #2: タンパク質・ペプチド | 分子量: 3433.953 Da / 分子数: 2 / 由来タイプ: 合成 詳細: The peptide was chemically synthesized. The sequence of the peptide is naturally found in Homo sapiens (human). 参照: UniProt: P01308 |
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-非ポリマー , 4種, 86分子
#3: 化合物 | #4: 化合物 | #5: 化合物 | #6: 水 | ChemComp-HOH / | |
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-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 2.54 Å3/Da / 溶媒含有率: 51.6 % |
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結晶化 | 温度: 290 K / pH: 6.2 詳細: Tris, sodium citrate, acetone, phenol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 6.20 |
-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: 回転陽極 / タイプ: BRUKER / 波長: 1.54 |
検出器 | タイプ: BRUKER / 検出器: CCD / 日付: 2004年7月23日 |
放射 | モノクロメーター: MIRRORS / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.54 Å / 相対比: 1 |
反射 | 解像度: 2.3→50.1 Å / Num. obs: 5346 / % possible obs: 97.7 % / Observed criterion σ(I): 0 / 冗長度: 12.9 % / Biso Wilson estimate: 39.7 Å2 / Rmerge(I) obs: 0.065 / Net I/σ(I): 9.7 |
反射 シェル | 解像度: 2.3→2.44 Å / 冗長度: 9.8 % / Rmerge(I) obs: 0.066 / Mean I/σ(I) obs: 9.6 / % possible all: 95.2 |
-解析
ソフトウェア |
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精密化 | 構造決定の手法: 分子置換 開始モデル: PDB ENTRY 1ZEG 解像度: 2.3→50.1 Å / Rfactor Rfree error: 0.009 / Isotropic thermal model: RESTRAINED / 交差検証法: THROUGHOUT / σ(F): 0 / 立体化学のターゲット値: ENGH & HUBER
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溶媒の処理 | 溶媒モデル: FLAT MODEL / Bsol: 71.24 Å2 / ksol: 0.38 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 46.9 Å2
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Refine analyze |
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精密化ステップ | サイクル: LAST / 解像度: 2.3→50.1 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 2.3→2.44 Å / Rfactor Rfree error: 0.013 / Total num. of bins used: 6
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