+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-9647 | |||||||||
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タイトル | Cryo-EM structure of a human intron lariat spliceosome after Prp43 loaded (ILS2 complex) at 2.9 angstrom resolution | |||||||||
マップデータ | The 2.86 angstrom map of human ILS2 complex | |||||||||
試料 |
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機能・相同性 | 機能・相同性情報 snRNP binding / U2 snRNP binding / post-mRNA release spliceosomal complex / U7 snRNA binding / histone pre-mRNA DCP binding / regulation of retinoic acid receptor signaling pathway / U7 snRNP / 3'-5' RNA helicase activity / histone pre-mRNA 3'end processing complex / generation of catalytic spliceosome for first transesterification step ...snRNP binding / U2 snRNP binding / post-mRNA release spliceosomal complex / U7 snRNA binding / histone pre-mRNA DCP binding / regulation of retinoic acid receptor signaling pathway / U7 snRNP / 3'-5' RNA helicase activity / histone pre-mRNA 3'end processing complex / generation of catalytic spliceosome for first transesterification step / regulation of vitamin D receptor signaling pathway / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / embryonic brain development / protein methylation / U12-type spliceosomal complex / methylosome / 7-methylguanosine cap hypermethylation / pre-mRNA binding / nuclear retinoic acid receptor binding / U2-type catalytic step 1 spliceosome / pICln-Sm protein complex / U1 snRNP binding / RNA splicing, via transesterification reactions / Prp19 complex / poly(A) binding / positive regulation of androgen receptor activity / spliceosomal tri-snRNP complex / small nuclear ribonucleoprotein complex / P granule / sno(s)RNA-containing ribonucleoprotein complex / ATP-dependent activity, acting on RNA / SMN-Sm protein complex / mRNA 3'-end processing / mRNA cis splicing, via spliceosome / U2-type spliceosomal complex / telomerase RNA binding / response to alkaloid / telomerase holoenzyme complex / positive regulation of mRNA splicing, via spliceosome / U2-type precatalytic spliceosome / U2-type prespliceosome assembly / commitment complex / U2-type catalytic step 2 spliceosome / U4 snRNP / positive regulation by host of viral transcription / Transport of Mature mRNA derived from an Intron-Containing Transcript / positive regulation of vitamin D receptor signaling pathway / U2 snRNP / Notch binding / nuclear vitamin D receptor binding / RNA Polymerase II Transcription Termination / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / U1 snRNP / RUNX3 regulates NOTCH signaling / U2-type prespliceosome / NOTCH4 Intracellular Domain Regulates Transcription / K63-linked polyubiquitin modification-dependent protein binding / ubiquitin-ubiquitin ligase activity / WD40-repeat domain binding / NOTCH3 Intracellular Domain Regulates Transcription / positive regulation of neurogenesis / lipid biosynthetic process / precatalytic spliceosome / nuclear androgen receptor binding / cyclosporin A binding / spliceosomal complex assembly / Notch-HLH transcription pathway / mRNA Splicing - Minor Pathway / Formation of paraxial mesoderm / positive regulation of transforming growth factor beta receptor signaling pathway / SMAD binding / protein K63-linked ubiquitination / mitotic G2 DNA damage checkpoint signaling / antiviral innate immune response / blastocyst development / protein localization to nucleus / spliceosomal tri-snRNP complex assembly / transcription-coupled nucleotide-excision repair / gastrulation / U5 snRNA binding / positive regulation of G1/S transition of mitotic cell cycle / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / U5 snRNP / retinoic acid receptor signaling pathway / embryonic organ development / positive regulation of viral genome replication / カハール体 / U2 snRNA binding / spliceosomal snRNP assembly / U6 snRNA binding / 転写後修飾 / protein peptidyl-prolyl isomerization / pre-mRNA intronic binding / U1 snRNA binding / cellular response to retinoic acid / U4/U6 x U5 tri-snRNP complex / catalytic step 2 spliceosome / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / mRNA Splicing - Major Pathway 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) / Human (ヒト) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.86 Å | |||||||||
データ登録者 | Zhang X / Zhan X / Yan C / Shi Y | |||||||||
資金援助 | 中国, 2件
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引用 | ジャーナル: Cell Res / 年: 2019 タイトル: Structures of the human spliceosomes before and after release of the ligated exon. 著者: Xiaofeng Zhang / Xiechao Zhan / Chuangye Yan / Wenyu Zhang / Dongliang Liu / Jianlin Lei / Yigong Shi / 要旨: Pre-mRNA splicing is executed by the spliceosome, which has eight major functional states each with distinct composition. Five of these eight human spliceosomal complexes, all preceding exon ...Pre-mRNA splicing is executed by the spliceosome, which has eight major functional states each with distinct composition. Five of these eight human spliceosomal complexes, all preceding exon ligation, have been structurally characterized. In this study, we report the cryo-electron microscopy structures of the human post-catalytic spliceosome (P complex) and intron lariat spliceosome (ILS) at average resolutions of 3.0 and 2.9 Å, respectively. In the P complex, the ligated exon remains anchored to loop I of U5 small nuclear RNA, and the 3'-splice site is recognized by the junction between the 5'-splice site and the branch point sequence. The ATPase/helicase Prp22, along with the ligated exon and eight other proteins, are dissociated in the P-to-ILS transition. Intriguingly, the ILS complex exists in two distinct conformations, one with the ATPase/helicase Prp43 and one without. Comparison of these three late-stage human spliceosomes reveals mechanistic insights into exon release and spliceosome disassembly. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_9647.map.gz | 226.6 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-9647-v30.xml emd-9647.xml | 51.3 KB 51.3 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_9647.png | 86.1 KB | ||
その他 | emd_9647_additional.map.gz | 193.2 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-9647 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-9647 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_9647.map.gz / 形式: CCP4 / 大きさ: 244.1 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | The 2.86 angstrom map of human ILS2 complex | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.338 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-追加マップ: The 4.0 angstrom map of the human ILS2 complex
ファイル | emd_9647_additional.map | ||||||||||||
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注釈 | The 4.0 angstrom map of the human ILS2 complex | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
+全体 : Human Intron Lariat Spliceosome
+超分子 #1: Human Intron Lariat Spliceosome
+分子 #1: Pre-mRNA-processing-splicing factor 8
+分子 #3: 116 kDa U5 small nuclear ribonucleoprotein component
+分子 #4: U5 small nuclear ribonucleoprotein 40 kDa protein
+分子 #6: Crooked neck-like protein 1
+分子 #7: Cell division cycle 5-like protein
+分子 #8: Pre-mRNA-splicing factor SYF2
+分子 #9: Protein BUD31 homolog
+分子 #10: Pre-mRNA-splicing factor RBM22
+分子 #11: Spliceosome-associated protein CWC15 homolog
+分子 #12: SNW domain-containing protein 1
+分子 #13: Peptidyl-prolyl cis-trans isomerase-like 1
+分子 #14: Pleiotropic regulator 1
+分子 #15: Pre-mRNA-processing factor 17
+分子 #18: CWF19-like protein 2
+分子 #19: Small nuclear ribonucleoprotein Sm D3
+分子 #20: Small nuclear ribonucleoprotein-associated protein
+分子 #21: Small nuclear ribonucleoprotein Sm D1
+分子 #22: Small nuclear ribonucleoprotein Sm D2
+分子 #23: Small nuclear ribonucleoprotein F
+分子 #24: Small nuclear ribonucleoprotein E
+分子 #25: Small nuclear ribonucleoprotein G
+分子 #26: Pre-mRNA-processing factor 19
+分子 #27: Pre-mRNA-splicing factor SPF27
+分子 #28: Pre-mRNA-splicing factor SYF1
+分子 #29: RNA helicase aquarius
+分子 #30: Peptidyl-prolyl cis-trans isomerase E
+分子 #31: U2 small nuclear ribonucleoprotein A'
+分子 #32: U2 small nuclear ribonucleoprotein B''
+分子 #33: Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
+分子 #2: U5snRNA
+分子 #5: U6snRNA
+分子 #16: pre-mRNA
+分子 #17: U2snRNA
+分子 #34: INOSITOL HEXAKISPHOSPHATE
+分子 #35: GUANOSINE-5'-TRIPHOSPHATE
+分子 #36: MAGNESIUM ION
+分子 #37: ZINC ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.9 |
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凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELDBright-field microscopy |
撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 45.0 e/Å2 |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
-画像解析
初期 角度割当 | タイプ: MAXIMUM LIKELIHOOD |
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最終 角度割当 | タイプ: MAXIMUM LIKELIHOOD |
最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 2.86 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 499840 |