National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
R01 AI056346
米国
National Natural Science Foundation of China (NSFC)
313290002
中国
National Institutes of Health/National Center for Research Resources (NIH/NCRR)
1S10RR23057
米国
China Academy of Sciences
XDB02050000
中国
National Science Foundation (NSF, United States)
DBI-1338135
米国
引用
ジャーナル: J Gen Virol / 年: 2017 タイトル: A pUL25 dimer interfaces the pseudorabies virus capsid and tegument. 著者: Yun-Tao Liu / Jiansen Jiang / Kevin Patrick Bohannon / Xinghong Dai / G W Gant Luxton / Wong Hoi Hui / Guo-Qiang Bi / Gregory Allan Smith / Z Hong Zhou / 要旨: Inside the virions of α-herpesviruses, tegument protein pUL25 anchors the tegument to capsid vertices through direct interactions with tegument proteins pUL17 and pUL36. In addition to promoting ...Inside the virions of α-herpesviruses, tegument protein pUL25 anchors the tegument to capsid vertices through direct interactions with tegument proteins pUL17 and pUL36. In addition to promoting virion assembly, both pUL25 and pUL36 are critical for intracellular microtubule-dependent capsid transport. Despite these essential roles during infection, the stoichiometry and precise organization of pUL25 and pUL36 on the capsid surface remain controversial due to the insufficient resolution of existing reconstructions from cryo-electron microscopy (cryoEM). Here, we report a three-dimensional (3D) icosahedral reconstruction of pseudorabies virus (PRV), a varicellovirus of the α-herpesvirinae subfamily, obtained by electron-counting cryoEM at 4.9 Å resolution. Our reconstruction resolves a dimer of pUL25 forming a capsid-associated tegument complex with pUL36 and pUL17 through a coiled coil helix bundle, thus correcting previous misinterpretations. A comparison between reconstructions of PRV and the γ-herpesvirus Kaposi's sarcoma-associated herpesvirus (KSHV) reinforces their similar architectures and establishes important subfamily differences in the capsid-tegument interface.
モデルのタイプ: INSILICO MODEL In silico モデル: The particles were processed using EMAN for initial 3D reconstruction and refinement with icosahedral symmetry enforced.
初期 角度割当
タイプ: NOT APPLICABLE
最終 角度割当
タイプ: NOT APPLICABLE
最終 再構成
想定した対称性 - 点群: I (正20面体型対称) / 解像度のタイプ: BY AUTHOR / 解像度: 4.9 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION / 使用した粒子像数: 13537