+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-6775 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
タイトル | Cryo-EM structure of human respiratory supercomplex I1III2IV1 | |||||||||
マップデータ | ||||||||||
試料 |
| |||||||||
機能・相同性 | 機能・相同性情報 subthalamus development / pons development / positive regulation of peptidase activity / cerebellar Purkinje cell layer development / protein insertion into mitochondrial inner membrane / Complex I biogenesis / blastocyst hatching / pyramidal neuron development / ubiquinone-6 biosynthetic process / TP53 Regulates Metabolic Genes ...subthalamus development / pons development / positive regulation of peptidase activity / cerebellar Purkinje cell layer development / protein insertion into mitochondrial inner membrane / Complex I biogenesis / blastocyst hatching / pyramidal neuron development / ubiquinone-6 biosynthetic process / TP53 Regulates Metabolic Genes / respiratory chain complex IV assembly / response to mercury ion / protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / thalamus development / mitochondrial respirasome assembly / Cytoprotection by HMOX1 / cellular response to oxygen levels / respiratory chain complex IV / Respiratory electron transport / iron-sulfur cluster assembly complex / mitochondrial respiratory chain complex III assembly / mitochondrial large ribosomal subunit binding / respiratory gaseous exchange by respiratory system / Mitochondrial protein import / regulation of oxidative phosphorylation / Respiratory electron transport / gliogenesis / mitochondrial respiratory chain complex III / neural precursor cell proliferation / mitochondrial respiratory chain complex IV / [2Fe-2S] cluster assembly / mitochondrial respirasome / NADH dehydrogenase activity / cardiac muscle tissue development / Glyoxylate metabolism and glycine degradation / response to alkaloid / oxygen sensor activity / 細胞呼吸 / シトクロムcオキシダーゼ / quinol-cytochrome-c reductase / ubiquinone binding / ubiquinol-cytochrome-c reductase activity / 酸化的リン酸化 / acyl binding / response to copper ion / oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor / response to glucagon / mitochondrial electron transport, cytochrome c to oxygen / mitochondrial ribosome / electron transport coupled proton transport / iron-sulfur cluster assembly / mitochondrial ATP synthesis coupled electron transport / cytochrome-c oxidase activity / mitochondrial electron transport, ubiquinol to cytochrome c / acyl carrier activity / azurophil granule membrane / mitochondrial translation / hypothalamus development / midbrain development / NADH:ユビキノン還元酵素 (水素イオン輸送型) / sodium ion transport / response to cobalamin / proton motive force-driven mitochondrial ATP synthesis / mitochondrial respiratory chain complex I / apoptotic mitochondrial changes / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / positive regulation of cysteine-type endopeptidase activity involved in apoptotic process / NADH dehydrogenase (ubiquinone) activity / RHOG GTPase cycle / quinone binding / response to hyperoxia / ATP synthesis coupled electron transport / cellular response to interferon-beta / animal organ regeneration / negative regulation of intrinsic apoptotic signaling pathway / enzyme regulator activity / response to cadmium ion / respirasome / 細胞呼吸 / ATP metabolic process / cellular response to retinoic acid / extrinsic apoptotic signaling pathway / response to cAMP / response to organonitrogen compound / ionotropic glutamate receptor binding / substantia nigra development / reactive oxygen species metabolic process / respiratory electron transport chain / cerebellum development / 神経発生 / response to activity / regulation of mitochondrial membrane potential / response to nicotine / synaptic membrane / generation of precursor metabolites and energy / central nervous system development / fatty acid binding / apoptotic signaling pathway 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.9 Å | |||||||||
データ登録者 | Gu J / Wu M / Yang M | |||||||||
引用 | ジャーナル: Cell / 年: 2017 タイトル: Architecture of Human Mitochondrial Respiratory Megacomplex IIIIIV. 著者: Runyu Guo / Shuai Zong / Meng Wu / Jinke Gu / Maojun Yang / 要旨: The respiratory megacomplex represents the highest-order assembly of respiratory chain complexes, and it allows mitochondria to respond to energy-requiring conditions. To understand its architecture, ...The respiratory megacomplex represents the highest-order assembly of respiratory chain complexes, and it allows mitochondria to respond to energy-requiring conditions. To understand its architecture, we examined the human respiratory chain megacomplex-IIIIIV (MCIIIIIV) with 140 subunits and a subset of associated cofactors using cryo-electron microscopy. The MCIIIIIV forms a circular structure with the dimeric CIII located in the center, where it is surrounded by two copies each of CI and CIV. Two cytochrome c (Cyt.c) molecules are positioned to accept electrons on the surface of the c state CIII dimer. Analyses indicate that CII could insert into the gaps between CI and CIV to form a closed ring, which we termed the electron transport chain supercomplex. The structure not only reveals the precise assignment of individual subunits of human CI and CIII, but also enables future in-depth analysis of the electron transport chain as a whole. | |||||||||
履歴 |
|
-構造の表示
ムービー |
ムービービューア |
---|---|
構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_6775.map.gz | 30.3 MB | EMDBマップデータ形式 | |
---|---|---|---|---|
ヘッダ (付随情報) | emd-6775-v30.xml emd-6775.xml | 17.5 KB 17.5 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_6775.png | 25.5 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-6775 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-6775 | HTTPS FTP |
-関連構造データ
関連構造データ | 5xthMC 6771C 6772C 6773C 6774C 6776C 5xtbC 5xtcC 5xtdC 5xteC 5xtiC M: このマップから作成された原子モデル C: 同じ文献を引用 (文献) |
---|---|
類似構造データ |
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
---|---|
「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_6775.map.gz / 形式: CCP4 / 大きさ: 421.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ボクセルのサイズ | X=Y=Z: 1.083 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
|
-添付データ
-試料の構成要素
-全体 : Human respiratory supercomplex I1III2IV1
全体 | 名称: Human respiratory supercomplex I1III2IV1 |
---|---|
要素 |
|
-超分子 #1: Human respiratory supercomplex I1III2IV1
超分子 | 名称: Human respiratory supercomplex I1III2IV1 / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#68 |
---|---|
由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 実験値: 1.7 MDa |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
---|---|
解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
濃度 | 0.2 mg/mL |
---|---|
緩衝液 | pH: 7.4 |
凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
---|---|
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: OTHER / 撮影モード: BRIGHT FIELDBright-field microscopy |
撮影 | フィルム・検出器のモデル: FEI FALCON II (4k x 4k) 平均電子線量: 1.25 e/Å2 |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
-画像解析
CTF補正 | ソフトウェア - 名称: CTFFIND (ver. 3.0) |
---|---|
初期 角度割当 | タイプ: RANDOM ASSIGNMENT / ソフトウェア - 名称: RELION (ver. 1.4) |
最終 角度割当 | タイプ: RANDOM ASSIGNMENT / ソフトウェア - 名称: RELION (ver. 1.4) |
最終 再構成 | 想定した対称性 - 点群: C1 (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 3.9 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION (ver. 1.4) / 使用した粒子像数: 167761 |