登録情報 データベース : EMDB / ID : EMD-31360 構造の表示 ダウンロードとリンクタイトル Cryo-EM structure of human GPR158 in complex with RGS7-Gbeta5 in a 2:1:1 ratio マップデータOverall refined map 詳細 試料複合体 : GPR158-RGS7-Gb5 in a 2:1:1 ratioタンパク質・ペプチド : Probable G-protein coupled receptor 158タンパク質・ペプチド : Regulator of G-protein signaling 7タンパク質・ペプチド : Guanine nucleotide-binding protein subunit beta-5 詳細機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
G protein-coupled glycine receptor activity / GTPase activator complex / 暗順応 / 暗順応 / G-protein gamma-subunit binding / negative regulation of voltage-gated calcium channel activity / negative regulation of G protein-coupled receptor signaling pathway / positive regulation of potassium ion transmembrane transport / regulation of G protein-coupled receptor signaling pathway / positive regulation of neurotransmitter secretion ... G protein-coupled glycine receptor activity / GTPase activator complex / 暗順応 / 暗順応 / G-protein gamma-subunit binding / negative regulation of voltage-gated calcium channel activity / negative regulation of G protein-coupled receptor signaling pathway / positive regulation of potassium ion transmembrane transport / regulation of G protein-coupled receptor signaling pathway / positive regulation of neurotransmitter secretion / dopamine receptor signaling pathway / regulation of synapse organization / enzyme activator activity / G-protein alpha-subunit binding / response to amphetamine / GTPase activator activity / cell projection / protein localization to plasma membrane / brain development / G beta:gamma signalling through PLC beta / Presynaptic function of Kainate receptors / Thromboxane signalling through TP receptor / G-protein activation / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / Prostacyclin signalling through prostacyclin receptor / G beta:gamma signalling through CDC42 / ADP signalling through P2Y purinoceptor 12 / G beta:gamma signalling through BTK / Adrenaline,noradrenaline inhibits insulin secretion / 認識 / Glucagon-type ligand receptors / Vasopressin regulates renal water homeostasis via Aquaporins / positive regulation of GTPase activity / G alpha (z) signalling events / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / ADORA2B mediated anti-inflammatory cytokines production / ADP signalling through P2Y purinoceptor 1 / G beta:gamma signalling through PI3Kgamma / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / GPER1 signaling / G-protein beta-subunit binding / heterotrimeric G-protein complex / Inactivation, recovery and regulation of the phototransduction cascade / G alpha (12/13) signalling events / transmembrane signaling receptor activity / signaling receptor complex adaptor activity / Thrombin signalling through proteinase activated receptors (PARs) / presynapse / presynaptic membrane / 核膜 / Ca2+ pathway / G alpha (i) signalling events / protein-folding chaperone binding / G alpha (s) signalling events / postsynaptic membrane / G alpha (q) signalling events / response to ethanol / Extra-nuclear estrogen signaling / neuron projection / intracellular signal transduction / G protein-coupled receptor signaling pathway / GTPase activity / シグナル伝達 / protein-containing complex / 細胞核 / 細胞膜 / 細胞質基質 / 細胞質 類似検索 - 分子機能 : / : / G-protein coupled receptor 158/179 / Regulator of G-protein signalling, DHEX domain / Regulator of G-protein signalling DHEX domain / Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP) / DEP domain profile. / Domain found in Dishevelled, Egl-10, and Pleckstrin / DEP domain / Regulator of G protein signaling domain ... : / : / G-protein coupled receptor 158/179 / Regulator of G-protein signalling, DHEX domain / Regulator of G-protein signalling DHEX domain / Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP) / DEP domain profile. / Domain found in Dishevelled, Egl-10, and Pleckstrin / DEP domain / Regulator of G protein signaling domain / RGS, subdomain 2 / RGS domain / RGS domain profile. / Regulator of G protein signalling domain / RGS domain superfamily / GPCR family 3, C-terminal / 7 transmembrane sweet-taste receptor of 3 GCPR / G-protein coupled receptors family 3 profile. / GGL domain / G-protein gamma-like domain superfamily / G-protein gamma-like domain / GGL domain / G protein gamma subunit-like motifs / Guanine nucleotide-binding protein, beta subunit / G-protein, beta subunit / G-protein beta WD-40 repeat / Winged helix DNA-binding domain superfamily / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / WD domain, G-beta repeat / WD40リピート / WD40リピート / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / Winged helix-like DNA-binding domain superfamily 類似検索 - ドメイン・相同性 Guanine nucleotide-binding protein subunit beta-5 / Regulator of G-protein signaling 7 / Metabotropic glycine receptor 類似検索 - 構成要素生物種 Homo sapiens (ヒト)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 4.3 Å 詳細 データ登録者Kim Y / Jeong E / Jeong J / Cho Y 資金援助 3件 詳細 詳細を隠すOrganization Grant number 国 National Research Foundation (NRF, Korea) 2021R1A2C301335711 National Research Foundation (NRF, Korea) 2017M3A9F6029736 Other private SSTF-BA1602-14
引用ジャーナル : Nat Commun / 年 : 2021タイトル : Structure of the class C orphan GPCR GPR158 in complex with RGS7-Gβ5.著者 : Eunyoung Jeong / Yoojoong Kim / Jihong Jeong / Yunje Cho / 要旨 : GPR158, a class C orphan GPCR, functions in cognition, stress-induced mood control, and synaptic development. Among class C GPCRs, GPR158 is unique as it lacks a Venus flytrap-fold ligand-binding ... GPR158, a class C orphan GPCR, functions in cognition, stress-induced mood control, and synaptic development. Among class C GPCRs, GPR158 is unique as it lacks a Venus flytrap-fold ligand-binding domain and terminates Gαi/o protein signaling through the RGS7-Gβ5 heterodimer. Here, we report the cryo-EM structures of GPR158 alone and in complex with one or two RGS7-Gβ5 heterodimers. GPR158 dimerizes through Per-Arnt-Sim-fold extracellular and transmembrane (TM) domains connected by an epidermal growth factor-like linker. The TM domain (TMD) reflects both inactive and active states of other class C GPCRs: a compact intracellular TMD, conformations of the two intracellular loops (ICLs) and the TMD interface formed by TM4/5. The ICL2, ICL3, TM3, and first helix of the cytoplasmic coiled-coil provide a platform for the DHEX domain of one RGS7 and the second helix recruits another RGS7. The unique features of the RGS7-binding site underlie the selectivity of GPR158 for RGS7. 履歴 登録 2021年5月25日 - ヘッダ(付随情報) 公開 2021年12月1日 - マップ公開 2021年12月1日 - 更新 2022年2月16日 - 現状 2022年2月16日 処理サイト : PDBj / 状態 : 公開
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