+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-31117 | |||||||||
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タイトル | TFIID lobe C subcomplex | |||||||||
マップデータ | TFIID lobe C subcomplex | |||||||||
試料 |
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機能・相同性 | 機能・相同性情報 spermine transport / negative regulation of MHC class I biosynthetic process / DNA-templated transcription open complex formation / TFIIH-class transcription factor complex binding / negative regulation of protein autoubiquitination / transcription factor TFTC complex / negative regulation of MHC class II biosynthetic process / regulation of cell cycle G1/S phase transition / RNA polymerase I general transcription initiation factor activity / SLIK (SAGA-like) complex ...spermine transport / negative regulation of MHC class I biosynthetic process / DNA-templated transcription open complex formation / TFIIH-class transcription factor complex binding / negative regulation of protein autoubiquitination / transcription factor TFTC complex / negative regulation of MHC class II biosynthetic process / regulation of cell cycle G1/S phase transition / RNA polymerase I general transcription initiation factor activity / SLIK (SAGA-like) complex / maintenance of protein location in nucleus / positive regulation of androgen receptor activity / transcription regulator inhibitor activity / SAGA complex / nuclear vitamin D receptor binding / RNA polymerase II general transcription initiation factor binding / nuclear thyroid hormone receptor binding / cellular response to ATP / regulation of fat cell differentiation / inner cell mass cell proliferation / histone acetyltransferase binding / midbrain development / RNA polymerase II general transcription initiation factor activity / transcription factor TFIID complex / HIV Transcription Initiation / RNA Polymerase II HIV Promoter Escape / Transcription of the HIV genome / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / ubiquitin conjugating enzyme activity / MLL1 complex / transcription initiation at RNA polymerase I promoter / aryl hydrocarbon receptor binding / RNA polymerase II transcribes snRNA genes / P-TEFb complex binding / negative regulation of cell cycle / positive regulation of transcription initiation by RNA polymerase II / RNA polymerase II core promoter sequence-specific DNA binding / intracellular estrogen receptor signaling pathway / regulation of DNA repair / positive regulation of intrinsic apoptotic signaling pathway / histone acetyltransferase activity / RNA polymerase II preinitiation complex assembly / negative regulation of ubiquitin-dependent protein catabolic process / ヒストンアセチルトランスフェラーゼ / RNA Polymerase II Pre-transcription Events / TBP-class protein binding / regulation of signal transduction by p53 class mediator / male germ cell nucleus / nuclear receptor binding / transcription initiation at RNA polymerase II promoter / DNA-templated transcription initiation / peptidyl-threonine phosphorylation / negative regulation of protein kinase activity / lysine-acetylated histone binding / mRNA transcription by RNA polymerase II / response to organic cyclic compound / protein polyubiquitination / cellular response to UV / G2/M transition of mitotic cell cycle / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / p53 binding / positive regulation of protein binding / kinase activity / ubiquitin-dependent protein catabolic process / peptidyl-serine phosphorylation / DNA-binding transcription factor binding / Regulation of TP53 Activity through Phosphorylation / transcription regulator complex / RNA polymerase II-specific DNA-binding transcription factor binding / sequence-specific DNA binding / transcription by RNA polymerase II / protein autophosphorylation / 細胞分化 / transcription coactivator activity / protein stabilization / transcription cis-regulatory region binding / non-specific serine/threonine protein kinase / protein kinase activity / 細胞周期 / positive regulation of apoptotic process / protein heterodimerization activity / negative regulation of cell population proliferation / negative regulation of gene expression / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / negative regulation of DNA-templated transcription / apoptotic process / DNA damage response / chromatin binding / クロマチン / regulation of transcription by RNA polymerase II / perinuclear region of cytoplasm / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / protein-containing complex 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.04 Å | |||||||||
データ登録者 | Chen X / Wu Z / Li J / Zhao D / Xu Y | |||||||||
引用 | ジャーナル: Science / 年: 2021 タイトル: Structural insights into preinitiation complex assembly on core promoters. 著者: Xizi Chen / Yilun Qi / Zihan Wu / Xinxin Wang / Jiabei Li / Dan Zhao / Haifeng Hou / Yan Li / Zishuo Yu / Weida Liu / Mo Wang / Yulei Ren / Ze Li / Huirong Yang / Yanhui Xu / 要旨: Transcription factor IID (TFIID) recognizes core promoters and supports preinitiation complex (PIC) assembly for RNA polymerase II (Pol II)-mediated eukaryotic transcription. We determined the ...Transcription factor IID (TFIID) recognizes core promoters and supports preinitiation complex (PIC) assembly for RNA polymerase II (Pol II)-mediated eukaryotic transcription. We determined the structures of human TFIID-based PIC in three stepwise assembly states and revealed two-track PIC assembly: stepwise promoter deposition to Pol II and extensive modular reorganization on track I (on TATA-TFIID-binding element promoters) versus direct promoter deposition on track II (on TATA-only and TATA-less promoters). The two tracks converge at an ~50-subunit holo PIC in identical conformation, whereby TFIID stabilizes PIC organization and supports loading of cyclin-dependent kinase (CDK)-activating kinase (CAK) onto Pol II and CAK-mediated phosphorylation of the Pol II carboxyl-terminal domain. Unexpectedly, TBP of TFIID similarly bends TATA box and TATA-less promoters in PIC. Our study provides structural visualization of stepwise PIC assembly on highly diversified promoters. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_31117.map.gz | 3.2 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-31117-v30.xml emd-31117.xml | 20.2 KB 20.2 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_31117.png | 107 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-31117 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-31117 | HTTPS FTP |
-関連構造データ
関連構造データ | 7eghMC 7edxC 7eg7C 7eg8C 7eg9C 7egaC 7egbC 7egcC 7egdC 7egeC 7egfC 7eggC 7egiC 7egjC M: このマップから作成された原子モデル C: 同じ文献を引用 (文献) |
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類似構造データ |
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_31117.map.gz / 形式: CCP4 / 大きさ: 512 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | TFIID lobe C subcomplex | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.055 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-試料の構成要素
-全体 : TFIID lobe C subcomplex
全体 | 名称: TFIID lobe C subcomplex |
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要素 |
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-超分子 #1: TFIID lobe C subcomplex
超分子 | 名称: TFIID lobe C subcomplex / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
組換発現 | 生物種: Homo sapiens (ヒト) |
-分子 #1: Transcription initiation factor TFIID subunit 1
分子 | 名称: Transcription initiation factor TFIID subunit 1 / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO EC番号: ヒストンアセチルトランスフェラーゼ |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 212.956172 KDa |
組換発現 | 生物種: Homo sapiens (ヒト) |
配列 | 文字列: MGPGCDLLLR TAATITAAAI MSDTDSDEDS AGGGPFSLAG FLFGNINGAG QLEGESVLDD ECKKHLAGLG ALGLGSLITE LTANEELTG TDGALVNDEG WVRSTEDAVD YSDINEVAED ESRRYQQTMG SLQPLCHSDY DEDDYDADCE DIDCKLMPPP P PPPGPMKK ...文字列: MGPGCDLLLR TAATITAAAI MSDTDSDEDS AGGGPFSLAG FLFGNINGAG QLEGESVLDD ECKKHLAGLG ALGLGSLITE LTANEELTG TDGALVNDEG WVRSTEDAVD YSDINEVAED ESRRYQQTMG SLQPLCHSDY DEDDYDADCE DIDCKLMPPP P PPPGPMKK DKDQDSITGE KVDFSSSSDS ESEMGPQEAT QAESEDGKLT LPLAGIMQHD ATKLLPSVTE LFPEFRPGKV LR FLRLFGP GKNVPSVWRS ARRKRKKKHR ELIQEEQIQE VECSVESEVS QKSLWNYDYA PPPPPEQCLS DDEITMMAPV ESK FSQSTG DIDKVTDTKP RVAEWRYGPA RLWYDMLGVP EDGSGFDYGF KLRKTEHEPV IKSRMIEEFR KLEENNGTDL LADE NFLMV TQLHWEDDII WDGEDVKHKG TKPQRASLAG WLPSSMTRNA MAYNVQQGFA ATLDDDKPWY SIFPIDNEDL VYGRW EDNI IWDAQAMPRL LEPPVLTLDP NDENLILEIP DEKEEATSNS PSKESKKESS LKKSRILLGK TGVIKEEPQQ NMSQPE VKD PWNLSNDEYY YPKQQGLRGT FGGNIIQHSI PAVELRQPFF PTHMGPIKLR QFHRPPLKKY SFGALSQPGP HSVQPLL KH IKKKAKMREQ ERQASGGGEM FFMRTPQDLT GKDGDLILAE YSEENGPLMM QVGMATKIKN YYKRKPGKDP GAPDCKYG E TVYCHTSPFL GSLHPGQLLQ AFENNLFRAP IYLHKMPETD FLIIRTRQGY YIRELVDIFV VGQQCPLFEV PGPNSKRAN THIRDFLQVF IYRLFWKSKD RPRRIRMEDI KKAFPSHSES SIRKRLKLCA DFKRTGMDSN WWVLKSDFRL PTEEEIRAMV SPEQCCAYY SMIAAEQRLK DAGYGEKSFF APEEENEEDF QMKIDDEVRT APWNTTRAFI AAMKGKCLLE VTGVADPTGC G EGFSYVKI PNKPTQQKDD KEPQPVKKTV TGTDADLRRL SLKNAKQLLR KFGVPEEEIK KLSRWEVIDV VRTMSTEQAR SG EGPMSKF ARGSRFSVAE HQERYKEECQ RIFDLQNKVL SSTEVLSTDT DSSSAEDSDF EEMGKNIENM LQNKKTSSQL SRE REEQER KELQRMLLAA GSAASGNNHR DDDTASVTSL NSSATGRCLK IYRTFRDEEG KEYVRCETVR KPAVIDAYVR IRTT KDEEF IRKFALFDEQ HREEMRKERR RIQEQLRRLK RNQEKEKLKG PPEKKPKKMK ERPDLKLKCG ACGAIGHMRT NKFCP LYYQ TNAPPSNPVA MTEEQEEELE KTVIHNDNEE LIKVEGTKIV LGKQLIESAD EVRRKSLVLK FPKQQLPPKK KRRVGT TVH CDYLNRPHKS IHRRRTDPMV TLSSILESII NDMRDLPNTY PFHTPVNAKV VKDYYKIITR PMDLQTLREN VRKRLYP SR EEFREHLELI VKNSATYNGP KHSLTQISQS MLDLCDEKLK EKEDKLARLE KAINPLLDDD DQVAFSFILD NIVTQKMM A VPDSWPFHHP VNKKFVPDYY KVIVNPMDLE TIRKNISKHK YQSRESFLDD VNLILANSVK YNGPESQYTK TAQEIVNVC YQTLTEYDEH LTQLEKDICT AKEAALEEAE LESLDPMTPG PYTPQPPDLY DTNTSLSMSR DASVFQDESN MSVLDIPSAT PEKQVTQEG EDGDGDLADE EEGTVQQPQA SVLYEDLLMS EGEDDEEDAG SDEEGDNPFS AIQLSESGSD SDVGSGGIRP K QPRMLQEN TRMDMENEES MMSYEGDGGE ASHGLEDSNI SYGSYEEPDP KSNTQDTSFS SIGGYEVSEE EEDEEEEEQR SG PSVLSQV HLSEDEEDSE DFHSIAGDSD LDSDE |
-分子 #2: Transcription initiation factor TFIID subunit 2
分子 | 名称: Transcription initiation factor TFIID subunit 2 / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 137.159984 KDa |
組換発現 | 生物種: Homo sapiens (ヒト) |
配列 | 文字列: MPLTGVEPAR MNRKKGDKGF ESPRPYKLTH QVVCINNINF QRKSVVGFVE LTIFPTVANL NRIKLNSKQC RIYRVRINDL EAAFIYNDP TLEVCHSESK QRNLNYFSNA YAAAVSAVDP DAGNGELCIK VPSELWKHVD ELKVLKIHIN FSLDQPKGGL H FVVPSVEG ...文字列: MPLTGVEPAR MNRKKGDKGF ESPRPYKLTH QVVCINNINF QRKSVVGFVE LTIFPTVANL NRIKLNSKQC RIYRVRINDL EAAFIYNDP TLEVCHSESK QRNLNYFSNA YAAAVSAVDP DAGNGELCIK VPSELWKHVD ELKVLKIHIN FSLDQPKGGL H FVVPSVEG SMAERGAHVF SCGYQNSTRF WFPCVDSYSE LCTWKLEFTV DAAMVAVSNG DLVETVYTHD MRKKTFHYML TI PTAASNI SLAIGPFEIL VDPYMHEVTH FCLPQLLPLL KHTTSYLHEV FEFYEEILTC RYPYSCFKTV FIDEAYVEVA AYA SMSIFS TNLLHSAMII DETPLTRRCL AQSLAQQFFG CFISRMSWSD EWVLKGISGY IYGLWMKKTF GVNEYRHWIK EELD KIVAY ELKTGGVLLH PIFGGGKEKD NPASHLHFSI KHPHTLSWEY YSMFQCKAHL VMRLIENRIS MEFMLQVFNK LLSLA STAS SQKFQSHMWS QMLVSTSGFL KSISNVSGKD IQPLIKQWVD QSGVVKFYGS FAFNRKRNVL ELEIKQDYTS PGTQKY VGP LKVTVQELDG SFNHTLQIEE NSLKHDIPCH SKSRRNKKKK IPLMNGEEVD MDLSAMDADS PLLWIRIDPD MSVLRKV EF EQADFMWQYQ LRYERDVVAQ QESILALEKF PTPASRLALT DILEQEQCFY RVRMSACFCL AKIANSMVST WTGPPAMK S LFTRMFCCKS CPNIVKTNNF MSFQSYFLQK TMPVAMALLR DVHNLCPKEV LTFILDLIKY NDNRKNKFSD NYYRAEMID ALANSVTPAV SVNNEVRTLD NLNPDVRLIL EEITRFLNME KLLPSYRHTI TVSCLRAIRV LQKNGHVPSD PALFKSYAEY GHFVDIRIA ALEAVVDYTK VDRSYEELQW LLNMIQNDPV PYVRHKILNM LTKNPPFTKN MESPLCNEAL VDQLWKLMNS G TSHDWRLR CGAVDLYFTL FGLSRPSCLP LPELGLVLNL KEKKAVLNPT IIPESVAGNQ EAANNPSSHP QLVGFQNPFS SS QDEEEID MDTVHDSQAF ISHHLNMLER PSTPGLSKYR PASSRSALIP QHSAGCDSTP TTKPQWSLEL ARKGTGKEQA PLE MSMHPA ASAPLSVFTK ESTASKHSDH HHHHHHEHKK KKKKHKHKHK HKHKHDSKEK DKEPFTFSSP ASGRSIRSPS LSD |
-分子 #3: Transcription initiation factor TFIID subunit 6
分子 | 名称: Transcription initiation factor TFIID subunit 6 / タイプ: protein_or_peptide / ID: 3 / コピー数: 2 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 72.749297 KDa |
組換発現 | 生物種: Homo sapiens (ヒト) |
配列 | 文字列: MAEEKKLKLS NTVLPSESMK VVAESMGIAQ IQEETCQLLT DEVSYRIKEI AQDALKFMHM GKRQKLTTSD IDYALKLKNV EPLYGFHAQ EFIPFRFASG GGRELYFYEE KEVDLSDIIN TPLPRVPLDV CLKAHWLSIE GCQPAIPENP PPAPKEQQKA E ATEPLKSA ...文字列: MAEEKKLKLS NTVLPSESMK VVAESMGIAQ IQEETCQLLT DEVSYRIKEI AQDALKFMHM GKRQKLTTSD IDYALKLKNV EPLYGFHAQ EFIPFRFASG GGRELYFYEE KEVDLSDIIN TPLPRVPLDV CLKAHWLSIE GCQPAIPENP PPAPKEQQKA E ATEPLKSA KPGQEEDGPL KGKGQGATTA DGKGKEKKAP PLLEGAPLRL KPRSIHELSV EQQLYYKEIT EACVGSCEAK RA EALQSIA TDPGLYQMLP RFSTFISEGV RVNVVQNNLA LLIYLMRMVK ALMDNPTLYL EKYVHELIPA VMTCIVSRQL CLR PDVDNH WALRDFAARL VAQICKHFST TTNNIQSRIT KTFTKSWVDE KTPWTTRYGS IAGLAELGHD VIKTLILPRL QQEG ERIRS VLDGPVLSNI DRIGADHVQS LLLKHCAPVL AKLRPPPDNQ DAYRAEFGSL GPLLCSQVVK ARAQAALQAQ QVNRT TLTI TQPRPTLTLS QAPQPGPRTP GLLKVPGSIA LPVQTLVSAR AAAPPQPSPP PTKFIVMSSS SSAPSTQQVL SLSTSA PGS GSTTTSPVTT TVPSVQPIVK LVSTATTAPP STAPSGPGSV QKYIVVSLPP TGEGKGGPTS HPSPVPPPAS SPSPLSG SA LCGGKQEAGD SPPPAPGTPK ANGSQPNSGS PQPAP |
-分子 #4: Transcription initiation factor TFIID subunit 7
分子 | 名称: Transcription initiation factor TFIID subunit 7 / タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 40.325117 KDa |
組換発現 | 生物種: Homo sapiens (ヒト) |
配列 | 文字列: MSKSKDDAPH ELESQFILRL PPEYASTVRR AVQSGHVNLK DRLTIELHPD GRHGIVRVDR VPLASKLVDL PCVMESLKTI DKKTFYKTA DICQMLVSTV DGDLYPPVEE PVASTDPKAS KKKDKDKEKK FIWNHGITLP LKNVRKRRFR KTAKKKYIES P DVEKEVKR ...文字列: MSKSKDDAPH ELESQFILRL PPEYASTVRR AVQSGHVNLK DRLTIELHPD GRHGIVRVDR VPLASKLVDL PCVMESLKTI DKKTFYKTA DICQMLVSTV DGDLYPPVEE PVASTDPKAS KKKDKDKEKK FIWNHGITLP LKNVRKRRFR KTAKKKYIES P DVEKEVKR LLSTDAEAVS TRWEIIAEDE TKEAENQGLD ISSPGMSGHR QGHDSLEHDE LREIFNDLSS SSEDEDETQH QD EEDINII DTEEDLERQL QDKLNESDEQ HQENEGTNQL VMGIQKQIDN MKGKLQETQD RAKRQEDLIM KVENLALKNR FQA VLDELK QKEDREKEQL SSLQEELESL LEK |
-分子 #5: Transcription initiation factor TFIID subunit 8
分子 | 名称: Transcription initiation factor TFIID subunit 8 / タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 34.304359 KDa |
組換発現 | 生物種: Homo sapiens (ヒト) |
配列 | 文字列: MADAAATAGA GGSGTRSGSK QSTNPADNYH LARRRTLQVV VSSLLTEAGF ESAEKASVET LTEMLQSYIS EIGRSAKSYC EHTARTQPT LSDIVVTLVE MGFNVDTLPA YAKRSQRMVI TAPPVTNQPV TPKALTAGQN RPHPPHIPSH FPEFPDPHTY I KTPTYREP ...文字列: MADAAATAGA GGSGTRSGSK QSTNPADNYH LARRRTLQVV VSSLLTEAGF ESAEKASVET LTEMLQSYIS EIGRSAKSYC EHTARTQPT LSDIVVTLVE MGFNVDTLPA YAKRSQRMVI TAPPVTNQPV TPKALTAGQN RPHPPHIPSH FPEFPDPHTY I KTPTYREP VSDYQVLREK AASQRRDVER ALTRFMAKTG ETQSLFKDDV STFPLIAARP FTIPYLTALL PSELEMQQME ET DSSEQDE QTDTENLALH ISMEDSGAEK ENTSVLQQNP SLSGSRNGEE NIIDNPYLRP VKKPKIRRKK SLS |
-分子 #6: DNA (45-MER)
分子 | 名称: DNA (45-MER) / タイプ: dna / ID: 6 / コピー数: 1 / 分類: DNA |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 22.317238 KDa |
配列 | 文字列: (DC)(DT)(DA)(DT)(DA)(DA)(DA)(DA)(DG)(DG) (DG)(DG)(DG)(DT)(DG)(DG)(DG)(DG)(DG)(DC) (DG)(DC)(DG)(DT)(DT)(DC)(DG)(DT)(DC) (DC)(DT)(DC)(DA)(DG)(DT)(DC)(DG)(DC)(DG) (DA) (DT)(DC)(DG)(DA)(DA) ...文字列: (DC)(DT)(DA)(DT)(DA)(DA)(DA)(DA)(DG)(DG) (DG)(DG)(DG)(DT)(DG)(DG)(DG)(DG)(DG)(DC) (DG)(DC)(DG)(DT)(DT)(DC)(DG)(DT)(DC) (DC)(DT)(DC)(DA)(DG)(DT)(DC)(DG)(DC)(DG) (DA) (DT)(DC)(DG)(DA)(DA)(DC)(DA)(DC) (DT)(DC)(DG)(DA)(DG)(DC)(DC)(DG)(DA)(DG) (DC)(DA) (DG)(DA)(DC)(DG)(DT)(DG)(DC) (DC)(DT)(DA)(DC)(DG) |
-分子 #7: DNA (45-MER)
分子 | 名称: DNA (45-MER) / タイプ: dna / ID: 7 / コピー数: 1 / 分類: DNA |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 28.814336 KDa |
配列 | 文字列: (DA)(DT)(DC)(DC)(DA)(DT)(DG)(DG)(DT)(DC) (DC)(DG)(DT)(DA)(DG)(DG)(DC)(DA)(DC)(DG) (DT)(DC)(DT)(DG)(DC)(DT)(DC)(DG)(DG) (DC)(DT)(DC)(DG)(DA)(DG)(DT)(DG)(DT)(DT) (DC) (DG)(DA)(DT)(DC)(DG) ...文字列: (DA)(DT)(DC)(DC)(DA)(DT)(DG)(DG)(DT)(DC) (DC)(DG)(DT)(DA)(DG)(DG)(DC)(DA)(DC)(DG) (DT)(DC)(DT)(DG)(DC)(DT)(DC)(DG)(DG) (DC)(DT)(DC)(DG)(DA)(DG)(DT)(DG)(DT)(DT) (DC) (DG)(DA)(DT)(DC)(DG)(DC)(DG)(DA) (DC)(DT)(DG)(DA)(DG)(DG)(DA)(DC)(DG)(DA) (DA)(DC) (DG)(DC)(DG)(DC)(DC)(DC)(DC) (DC)(DA)(DC)(DC)(DC)(DC)(DC)(DT)(DT)(DT) (DT)(DA)(DT) (DA)(DG)(DG)(DC)(DG)(DC) (DC)(DC)(DT)(DT)(DC)(DG)(DA)(DT) |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.9 |
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グリッド | 材質: GOLD / 前処理 - タイプ: PLASMA CLEANING |
凍結 | 凍結剤: ETHANE / 装置: FEI VITROBOT MARK IV |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 検出モード: SUPER-RESOLUTION / 平均電子線量: 50.0 e/Å2 |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
-画像解析
初期 角度割当 | タイプ: OTHER |
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最終 角度割当 | タイプ: MAXIMUM LIKELIHOOD |
最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 3.04 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 121285 |
-原子モデル構築 1
精密化 | 空間: REAL / プロトコル: AB INITIO MODEL |
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得られたモデル | PDB-7egh: |