+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-10775 | ||||||||||||
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タイトル | Structure of a human 48S translational initiation complex - 40S body | ||||||||||||
マップデータ | |||||||||||||
試料 |
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キーワード | eIF3 (EIF3) / ribosome (リボソーム) / translation (翻訳 (生物学)) / initiation complex (イニシエーション) | ||||||||||||
機能・相同性 | 機能・相同性情報 positive regulation of mRNA binding / positive regulation of mRNA cis splicing, via spliceosome / multi-eIF complex / eukaryotic translation initiation factor 3 complex / eukaryotic 43S preinitiation complex / translation factor activity, RNA binding / formation of cytoplasmic translation initiation complex / eukaryotic 48S preinitiation complex / negative regulation of RNA splicing / laminin receptor activity ...positive regulation of mRNA binding / positive regulation of mRNA cis splicing, via spliceosome / multi-eIF complex / eukaryotic translation initiation factor 3 complex / eukaryotic 43S preinitiation complex / translation factor activity, RNA binding / formation of cytoplasmic translation initiation complex / eukaryotic 48S preinitiation complex / negative regulation of RNA splicing / laminin receptor activity / neural crest cell differentiation / rRNA modification in the nucleus and cytosol / positive regulation of ubiquitin-protein transferase activity / Formation of the ternary complex, and subsequently, the 43S complex / regulation of translational initiation / erythrocyte homeostasis / cytoplasmic side of rough endoplasmic reticulum membrane / ubiquitin ligase inhibitor activity / negative regulation of ubiquitin protein ligase activity / Ribosomal scanning and start codon recognition / Translation initiation complex formation / fibroblast growth factor binding / SARS-CoV-1 modulates host translation machinery / ヒドロキシル化 / MTOR / mTORC1-mediated signalling / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / Peptide chain elongation / Selenocysteine synthesis / Formation of a pool of free 40S subunits / ribosomal small subunit binding / Eukaryotic Translation Termination / Response of EIF2AK4 (GCN2) to amino acid deficiency / translation regulator activity / SRP-dependent cotranslational protein targeting to membrane / Viral mRNA Translation / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / Major pathway of rRNA processing in the nucleolus and cytosol / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / 小胞体 / laminin binding / Protein methylation / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Nuclear events stimulated by ALK signaling in cancer / positive regulation of cell cycle / stress granule assembly / translation initiation factor binding / maturation of SSU-rRNA / Mitotic Prometaphase / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / EML4 and NUDC in mitotic spindle formation / negative regulation of ubiquitin-dependent protein catabolic process / translational initiation / Resolution of Sister Chromatid Cohesion / ribosome assembly / translation initiation factor activity / small-subunit processome / cytosolic ribosome / 赤血球形成 / positive regulation of translation / innate immune response in mucosa / mRNA 3'-UTR binding / neural tube closure / RHO GTPases Activate Formins / maintenance of translational fidelity / response to virus / mRNA 5'-UTR binding / ribosomal small subunit biogenesis / cytoplasmic ribonucleoprotein granule / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / RMTs methylate histone arginines / rRNA processing / Regulation of expression of SLITs and ROBOs / Separation of Sister Chromatids / cytosolic small ribosomal subunit / antimicrobial humoral immune response mediated by antimicrobial peptide / ribosome binding / glucose homeostasis / virus receptor activity / cell body / cytoplasmic translation / small ribosomal subunit / cytosolic large ribosomal subunit / antibacterial humoral response / SARS-CoV-2 modulates host translation machinery / tRNA binding / postsynaptic density / 細胞分化 / rRNA binding / protein stabilization / リボソーム / structural constituent of ribosome / defense response to Gram-positive bacterium / cadherin binding / ribonucleoprotein complex 類似検索 - 分子機能 | ||||||||||||
生物種 | Homo sapiens (ヒト) | ||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.1 Å | ||||||||||||
データ登録者 | Brito Querido J / Sokabe M | ||||||||||||
資金援助 | 英国, 米国, 3件
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引用 | ジャーナル: Science / 年: 2020 タイトル: Structure of a human 48 translational initiation complex. 著者: Jailson Brito Querido / Masaaki Sokabe / Sebastian Kraatz / Yuliya Gordiyenko / J Mark Skehel / Christopher S Fraser / V Ramakrishnan / 要旨: A key step in translational initiation is the recruitment of the 43 preinitiation complex by the cap-binding complex [eukaryotic initiation factor 4F (eIF4F)] at the 5' end of messenger RNA (mRNA) to ...A key step in translational initiation is the recruitment of the 43 preinitiation complex by the cap-binding complex [eukaryotic initiation factor 4F (eIF4F)] at the 5' end of messenger RNA (mRNA) to form the 48 initiation complex (i.e., the 48). The 48 then scans along the mRNA to locate a start codon. To understand the mechanisms involved, we used cryo-electron microscopy to determine the structure of a reconstituted human 48 The structure reveals insights into early events of translation initiation complex assembly, as well as how eIF4F interacts with subunits of eIF3 near the mRNA exit channel in the 43 The location of eIF4F is consistent with a slotting model of mRNA recruitment and suggests that downstream mRNA is unwound at least in part by being "pulled" through the 40 subunit during scanning. | ||||||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_10775.map.gz | 36.6 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-10775-v30.xml emd-10775.xml | 50.7 KB 50.7 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_10775_fsc.xml | 17.7 KB | 表示 | FSCデータファイル |
画像 | emd_10775.png | 168.4 KB | ||
Filedesc metadata | emd-10775.cif.gz | 11 KB | ||
その他 | emd_10775_additional_1.map.gz emd_10775_additional_2.map.gz emd_10775_half_map_1.map.gz emd_10775_half_map_2.map.gz | 382 MB 391.4 MB 394 MB 393.9 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-10775 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-10775 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_10775.map.gz / 形式: CCP4 / 大きさ: 476.8 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.074 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-追加マップ: #1
ファイル | emd_10775_additional_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-追加マップ: #2
ファイル | emd_10775_additional_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_10775_half_map_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #2
ファイル | emd_10775_half_map_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
+全体 : Human 48S initiation complex
+超分子 #1: Human 48S initiation complex
+超分子 #2: Human 48S initiation complex
+超分子 #3: Human 48S initiation complex
+超分子 #4: Human 48S initiation complex
+分子 #1: 40S ribosomal protein S4, X isoform
+分子 #2: 40S ribosomal protein S11
+分子 #3: 40S ribosomal protein S23
+分子 #4: 40S ribosomal protein S9
+分子 #5: 40S ribosomal protein S7
+分子 #6: 40S ribosomal protein S30
+分子 #7: 40S ribosomal protein S27
+分子 #8: 40S ribosomal protein S21
+分子 #9: 40S ribosomal protein S15a
+分子 #10: 40S ribosomal protein S17
+分子 #11: 40S ribosomal protein S2
+分子 #12: 40S ribosomal protein S3a
+分子 #13: 40S ribosomal protein SA
+分子 #14: 40S ribosomal protein S26
+分子 #15: 40S ribosomal protein S14
+分子 #16: 40S ribosomal protein S6
+分子 #17: 40S ribosomal protein S8
+分子 #18: 40S ribosomal protein S24
+分子 #19: Eukaryotic translation initiation factor 1A, X-chromosomal
+分子 #20: Eukaryotic translation initiation factor 1
+分子 #21: 40S ribosomal protein S13
+分子 #22: Eukaryotic translation initiation factor 3 subunit J
+分子 #23: Eukaryotic translation initiation factor 3 subunit C
+分子 #24: 60S ribosomal protein L41
+分子 #25: 18S rRNA
+分子 #26: mRNA
+分子 #27: MAGNESIUM ION
+分子 #28: ZINC ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELDBright-field microscopy |
撮影 | フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 平均露光時間: 1.0 sec. / 平均電子線量: 107.0 e/Å2 |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |