+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-0013 | |||||||||
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タイトル | Helical reconstruction of BCL10 CARD and MALT1 DEATH DOMAIN complex | |||||||||
マップデータ | ||||||||||
試料 |
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機能・相同性 | 機能・相同性情報 positive regulation of lymphotoxin A production / polkadots / B-1 B cell differentiation / positive regulation of T-helper 17 cell differentiation / regulation of T cell receptor signaling pathway / CBM complex / antifungal innate immune response / protein kinase B binding / response to fungus / T cell apoptotic process ...positive regulation of lymphotoxin A production / polkadots / B-1 B cell differentiation / positive regulation of T-helper 17 cell differentiation / regulation of T cell receptor signaling pathway / CBM complex / antifungal innate immune response / protein kinase B binding / response to fungus / T cell apoptotic process / positive regulation of mast cell cytokine production / CARD domain binding / プログラム細胞死 / negative regulation of mature B cell apoptotic process / B cell apoptotic process / activation of NF-kappaB-inducing kinase activity / CLEC7A/inflammasome pathway / 核外搬出シグナル / response to food / positive regulation of extrinsic apoptotic signaling pathway / Toll様受容体 / B cell activation / small molecule binding / positive regulation of cysteine-type endopeptidase activity involved in apoptotic process / positive regulation of T cell receptor signaling pathway / immunoglobulin mediated immune response / general transcription initiation factor binding / 免疫シナプス / NF-kappaB binding / cellular defense response / cytoplasmic microtubule / T cell proliferation / positive regulation of phosphorylation / lipopolysaccharide-mediated signaling pathway / proteolysis involved in protein catabolic process / positive regulation of interleukin-2 production / canonical NF-kappaB signal transduction / positive regulation of interleukin-1 beta production / positive regulation of protein ubiquitination / neural tube closure / positive regulation of interleukin-8 production / Activation of NF-kappaB in B cells / protein homooligomerization / defense response / 核小体 / CLEC7A (Dectin-1) signaling / positive regulation of T cell cytokine production / FCERI mediated NF-kB activation / positive regulation of interleukin-6 production / cellular response to mechanical stimulus / ubiquitin-protein transferase activity / protein self-association / positive regulation of T cell activation / Downstream TCR signaling / E3 ubiquitin ligases ubiquitinate target proteins / positive regulation of NF-kappaB transcription factor activity / T cell receptor signaling pathway / peptidase activity / regulation of apoptotic process / positive regulation of canonical NF-kappaB signal transduction / endopeptidase activity / cellular response to lipopolysaccharide / protease binding / 加水分解酵素; プロテアーゼ; ペプチド結合加水分解酵素; システインプロテアーゼ / 獲得免疫系 / リソソーム / transcription coactivator activity / positive regulation of apoptotic process / 脂質ラフト / cysteine-type endopeptidase activity / 自然免疫系 / ubiquitin protein ligase binding / protein-containing complex binding / negative regulation of apoptotic process / perinuclear region of cytoplasm / positive regulation of DNA-templated transcription / protein-containing complex / タンパク質分解 / 細胞核 / identical protein binding / 細胞質基質 / 細胞質 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | らせん対称体再構成法 / クライオ電子顕微鏡法 / 解像度: 4.9 Å | |||||||||
データ登録者 | Schlauderer F / Desfosses A / Gutsche I / Hopfner KP / Lammens K | |||||||||
引用 | ジャーナル: Nat Commun / 年: 2018 タイトル: Molecular architecture and regulation of BCL10-MALT1 filaments. 著者: Florian Schlauderer / Thomas Seeholzer / Ambroise Desfosses / Torben Gehring / Mike Strauss / Karl-Peter Hopfner / Irina Gutsche / Daniel Krappmann / Katja Lammens / 要旨: The CARD11-BCL10-MALT1 (CBM) complex triggers the adaptive immune response in lymphocytes and lymphoma cells. CARD11/CARMA1 acts as a molecular seed inducing BCL10 filaments, but the integration of ...The CARD11-BCL10-MALT1 (CBM) complex triggers the adaptive immune response in lymphocytes and lymphoma cells. CARD11/CARMA1 acts as a molecular seed inducing BCL10 filaments, but the integration of MALT1 and the assembly of a functional CBM complex has remained elusive. Using cryo-EM we solved the helical structure of the BCL10-MALT1 filament. The structural model of the filament core solved at 4.9 Å resolution identified the interface between the N-terminal MALT1 DD and the BCL10 caspase recruitment domain. The C-terminal MALT1 Ig and paracaspase domains protrude from this core to orchestrate binding of mediators and substrates at the filament periphery. Mutagenesis studies support the importance of the identified BCL10-MALT1 interface for CBM complex assembly, MALT1 protease activation and NF-κB signaling in Jurkat and primary CD4 T-cells. Collectively, we present a model for the assembly and architecture of the CBM signaling complex and how it functions as a signaling hub in T-lymphocytes. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_0013.map.gz | 66.7 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-0013-v30.xml emd-0013.xml | 15.7 KB 15.7 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_0013_fsc.xml | 11.4 KB | 表示 | FSCデータファイル |
画像 | emd_0013.png | 274.1 KB | ||
その他 | emd_0013_half_map_1.map.gz emd_0013_half_map_2.map.gz | 46 MB 46 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-0013 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-0013 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_0013.map.gz / 形式: CCP4 / 大きさ: 91.6 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.002 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-ハーフマップ: Unmasked, unfiltered, helically symmetrized half map 2
ファイル | emd_0013_half_map_1.map | ||||||||||||
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注釈 | Unmasked, unfiltered, helically symmetrized half map 2 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Unmasked, unfiltered, helically symmetrized half map 1
ファイル | emd_0013_half_map_2.map | ||||||||||||
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注釈 | Unmasked, unfiltered, helically symmetrized half map 1 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
-全体 : Complex of BCL10 CARD and MALT1 DEATH DOMAIN
全体 | 名称: Complex of BCL10 CARD and MALT1 DEATH DOMAIN |
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要素 |
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-超分子 #1: Complex of BCL10 CARD and MALT1 DEATH DOMAIN
超分子 | 名称: Complex of BCL10 CARD and MALT1 DEATH DOMAIN / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
組換発現 | 生物種: Escherichia coli (大腸菌) |
分子量 | 実験値: 104 kDa/nm |
-分子 #1: B-cell lymphoma/leukemia 10
分子 | 名称: B-cell lymphoma/leukemia 10 / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 12.614566 KDa |
組換発現 | 生物種: Escherichia coli (大腸菌) |
配列 | 文字列: EEDLTEVKKD ALENLRVYLC EKIIAERHFD HLRAKKILSR EDTEEISCRT SSRKRAGKLL DYLQENPKGL DTLVESIRRE KTQNFLIQK ITDEVLKLRN IKLEHLK |
-分子 #2: Mucosa-associated lymphoid tissue lymphoma translocation protein 1
分子 | 名称: Mucosa-associated lymphoid tissue lymphoma translocation protein 1 タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO EC番号: 加水分解酵素; プロテアーゼ; ペプチド結合加水分解酵素; システインプロテアーゼ |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 10.401128 KDa |
組換発現 | 生物種: Escherichia coli (大腸菌) |
配列 | 文字列: LNRLREPLLR RLSELLDQAP EGRGWRRLAE LAGSRGRLRL SCLDLEQCSL KVLEPEGSPS LCLLKLMGEK GCTVTELSDF LQAMEHTEV LQL |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | らせん対称体再構成法 |
試料の集合状態 | filament |
-試料調製
濃度 | 1 mg/mL |
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緩衝液 | pH: 7.5 |
グリッド | モデル: Quantifoil R1.2/1.3 / 材質: COPPER |
凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TECNAI ARCTICA |
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電子線 | 加速電圧: 200 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELDBright-field microscopy |
撮影 | フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 検出モード: INTEGRATING / 平均電子線量: 99.6 e/Å2 |
実験機器 | モデル: Talos Arctica / 画像提供: FEI Company |