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- PDB-8pjh: Crystal structure of human insulin desB30 precursor with an Aspar... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8pjh | ||||||
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Title | Crystal structure of human insulin desB30 precursor with an Aspartate-Glycine-Lysine C-peptide in dimer (T2) conformation | ||||||
![]() | Insulin | ||||||
![]() | HORMONE / insulin / precursor / dimer | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Schluckebier, G. / Johansson, E. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Molecular engineering of insulin for recombinant expression in yeast. Authors: Kjeldsen, T. / Andersen, A.S. / Hubalek, F. / Johansson, E. / Kreiner, F.F. / Schluckebier, G. / Kurtzhals, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 53.8 KB | Display | ![]() |
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PDB format | ![]() | 32.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 8pi4C ![]() 8pi5C ![]() 8pi6C ![]() 8pjcC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 5999.851 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.31 Å3/Da / Density % sol: 62.82 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / Details: TBD |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Feb 9, 2004 |
Radiation | Monochromator: mirror / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→99 Å / Num. obs: 72811 / % possible obs: 99.2 % / Redundancy: 5.7 % / Biso Wilson estimate: 23.04 Å2 / Rmerge(I) obs: 0.048 / Χ2: 1.26 / Net I/av σ(I): 15.7 / Net I/σ(I): 45.7 |
Reflection shell | Resolution: 1.5→1.55 Å / Redundancy: 5.4 % / Rmerge(I) obs: 0.768 / Mean I/σ(I) obs: 2.2 / Num. unique obs: 1250 / Χ2: 1.09 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 32.13 Å2 | ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.5→14.26 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -19.2391038276 Å / Origin y: -0.765479047723 Å / Origin z: 9.15692256171 Å
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Refinement TLS group | Selection details: all |