cholesterol 24-hydroxylase / cholesterol 24-hydroxylase activity / protein localization to membrane raft / testosterone 16-beta-hydroxylase activity / Synthesis of bile acids and bile salts via 24-hydroxycholesterol / progesterone metabolic process / sterol metabolic process / bile acid biosynthetic process / regulation of long-term synaptic potentiation / steroid hydroxylase activity ...cholesterol 24-hydroxylase / cholesterol 24-hydroxylase activity / protein localization to membrane raft / testosterone 16-beta-hydroxylase activity / Synthesis of bile acids and bile salts via 24-hydroxycholesterol / progesterone metabolic process / sterol metabolic process / bile acid biosynthetic process / regulation of long-term synaptic potentiation / steroid hydroxylase activity / Endogenous sterols / cholesterol catabolic process / xenobiotic metabolic process / presynapse / nervous system development / postsynapse / iron ion binding / dendrite / endoplasmic reticulum membrane / heme binding / endoplasmic reticulum 類似検索 - 分子機能
解像度: 2.4→46.21 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 4160886 / Data cutoff low absF: 0 / Isotropic thermal model: variable / 交差検証法: THROUGHOUT / σ(F): 0 / σ(I): 0 / 立体化学のターゲット値: Engh & Huber 詳細: Refmac5 and TLS used in intermediate refinement steps. TLS not used in CNS refinement. TLS groups determined using the TLSMD server. TLSMD REF: J Painter & E A Merritt (2006) J. Appl. Cryst. ...詳細: Refmac5 and TLS used in intermediate refinement steps. TLS not used in CNS refinement. TLS groups determined using the TLSMD server. TLSMD REF: J Painter & E A Merritt (2006) J. Appl. Cryst. 39, 109-111. PMB used in all CNS refinements. PMB BOND target set to 0.011. PMB Variable Sigma-B used default parameters. PMB Hydrogen bond NOE restraints used: D=2.90, 0.4, 0.7, K=75, PMB Hydrogen bond NOE restraints used: Number of NOEs=272. PMB Local scale reject set to 35 sigma, PMB Local Scale rejected 103 reflections of 21096. PMB Local Scale (h,k,l) box sizes = +/- 4 3 5, PMB Local Scale maximum scale used (localscale method): 32.710, PMB Local Scale minimum scale used (localscale method): 1.053. Note: WEAK DENSITY OVER HEME WAS NOT MODELLED. IT COULD BE A HISTIDINE FROM the BUFFER OR IT COULD BE DUE TO ANOTHER UNKNOWN MOLECULE FROM EXPRESSION. Note: The F'-G' LOOP, residues 229-239, was not built due to weak and ambiguous electron density