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- EMDB-31665: map4,one conformation of the IGF1R/insulin complex -

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Basic information

Entry
Database: EMDB / ID: EMD-31665
Titlemap4,one conformation of the IGF1R/insulin complex
Map datamap4, one conformation of the IGF1R/insulin complex
Sample
  • Complex: Complex of the dimer IGF1R with insulin
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.1 Å
AuthorsZhang J / Liu C / Zhang X / Wei T / Wu C
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)81972766 China
CitationJournal: To Be Published
Title: map4,one conformation of the IGF1R/insulin complex
Authors: Zhang J / Liu C / Zhang X / Wei T / Wu C
History
DepositionAug 9, 2021-
Header (metadata) releaseAug 17, 2022-
Map releaseAug 17, 2022-
UpdateAug 17, 2022-
Current statusAug 17, 2022Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_31665.png

Downloads & links

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Map

FileDownload / File: emd_31665.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationmap4, one conformation of the IGF1R/insulin complex
Voxel sizeX=Y=Z: 1.08 Å
Density
Contour LevelBy AUTHOR: 0.15
Minimum - Maximum-0.9017461 - 1.5676773
Average (Standard dev.)0.0029820662 (±0.033507813)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 345.6 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Complex of the dimer IGF1R with insulin

EntireName: Complex of the dimer IGF1R with insulin
Components
  • Complex: Complex of the dimer IGF1R with insulin

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Supramolecule #1: Complex of the dimer IGF1R with insulin

SupramoleculeName: Complex of the dimer IGF1R with insulin / type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1.5 mg/mL
BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 50.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: OTHER
Final angle assignmentType: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 133307
FSC plot (resolution estimation)

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