EMDB-13611: The acetogenin-bound complex I of Mus musculus resolved to 3.4 an...

基本情報

登録情報

データベース: EMDB / ID: EMD-13611

• タイトル: The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms
• マップデータ: Globally sharpened map (postprocessed) in Relion

試料

• 複合体: Mitochondrial NADH:ubiquinone oxidoreductase from Mus musculus
  • タンパク質・ペプチド: x 44種
• リガンド: x 11種

機能・相同性

分子機能:

Mitochondrial protein import / response to injury involved in regulation of muscle adaptation / Protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / Complex I biogenesis / RHOG GTPase cycle / : / Respiratory electron transport / protein insertion into mitochondrial inner membrane / blastocyst hatching / mesenchymal stem cell proliferation / reproductive system development / respiratory system process / psychomotor behavior / Mitochondrial protein degradation / cellular response to oxygen levels / response to light intensity / respiratory chain complex / mesenchymal stem cell differentiation / circulatory system development / mitochondrial [2Fe-2S] assembly complex / iron-sulfur cluster assembly complex / mitochondrial large ribosomal subunit binding / gliogenesis / negative regulation of non-canonical NF-kappaB signal transduction / cardiac muscle tissue development / neural precursor cell proliferation / [2Fe-2S] cluster assembly / positive regulation of mitochondrial membrane potential / stem cell division / adult walking behavior / oxygen sensor activity / response to hydroperoxide / cellular response to glucocorticoid stimulus / iron-sulfur cluster assembly / dopamine metabolic process / NADH:ubiquinone reductase (H+-translocating) / adult behavior / positive regulation of ATP biosynthetic process / mitochondrial respiratory chain complex I assembly / NADH dehydrogenase activity / mitochondrial electron transport, NADH to ubiquinone / proton motive force-driven mitochondrial ATP synthesis / positive regulation of execution phase of apoptosis / respiratory chain complex I / NADH dehydrogenase (ubiquinone) activity / neuron development / quinone binding / cellular response to interferon-beta / : / ATP synthesis coupled electron transport / extrinsic apoptotic signaling pathway / negative regulation of reactive oxygen species biosynthetic process / cellular response to retinoic acid / tricarboxylic acid cycle / neurogenesis / Neutrophil degranulation / visual perception / muscle contraction / cerebellum development / reactive oxygen species metabolic process / aerobic respiration / regulation of mitochondrial membrane potential / respiratory electron transport chain / DNA damage response, signal transduction by p53 class mediator / mitochondrion organization / fatty acid metabolic process / kidney development / response to nicotine / response to cocaine / sensory perception of sound / response to hydrogen peroxide / monooxygenase activity / electron transport chain / brain development / mitochondrial membrane / mitochondrial intermembrane space / multicellular organism growth / 2 iron, 2 sulfur cluster binding / cognition / circadian rhythm / positive regulation of protein catabolic process / NAD binding / fatty acid biosynthetic process / cellular senescence / FMN binding / nervous system development / myelin sheath / 4 iron, 4 sulfur cluster binding / neuron apoptotic process / response to ethanol / gene expression / in utero embryonic development / response to oxidative stress / response to hypoxia / electron transfer activity / mitochondrial inner membrane / nuclear speck / nuclear body / mitochondrial matrix

ドメイン・相同性:

NmrA-like domain / NmrA-like family / Complex1_LYR-like / NADH-ubiquinone oxidoreductase 1 subunit C1 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH:ubiquinone oxidoreductase, ESSS subunit / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, NDUB1 / MNLL subunit / NADH-ubiquinone oxidoreductase flavoprotein 3 / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / Tim17/Tim22/Tim23/Pmp24 family / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, metazoa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, animal type / NADH dehydrogenase 1 beta subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUC2 / NADH:ubiquinone oxidoreductase, subunit NDUFB4 / NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2) / NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4) / NADH dehydrogenase 1, beta subcomplex, subunit 6 / NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit / : / NADH:ubiquinone oxidoreductase chain 4, N-terminal / NADH:ubiquinone oxidoreductase, chain 2 / NADH dehydrogenase subunit 2, C-terminal / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / : / : / NADH-ubiquinone oxidoreductase chain 4, amino terminus / NADH dehydrogenase subunit 2 C-terminus / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / : / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase 1 alpha subcomplex subunit 3 / Zinc finger, CHCC-type / Zinc-finger domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NADH:ubiquinone oxidoreductase, NDUFS5-15kDa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / GRIM-19 / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NDUFB9, LYR domain / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / NDUFA6, LYR domain / Deoxynucleoside kinase domain / Deoxynucleoside kinase / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / : / SLBB domain / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / : / : / NADH-quinone oxidoreductase subunit 3, ferredoxin-like domain / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / NADH-quinone oxidoreductase, chain M/4 / NADH-ubiquinone oxidoreductase chain 4L/K / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / NAD(P)H-quinone oxidoreductase subunit D/H / NADH-quinone oxidoreductase, chain 5-like / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like / NADH-ubiquinone/plastoquinone oxidoreductase chain 4L / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3.

構成要素:

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial / NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 subunit C2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / Acyl carrier protein, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

• 生物種: Mus musculus (ハツカネズミ) / house mouse (ハツカネズミ) / Mouse (ネズミ)
• 手法: 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.4 Å
• データ登録者: Grba D / Hirst J

資金援助

英国, 2件

Organization	Grant number	国
Medical Research Council (MRC, United Kingdom)	MC_U105663141	英国
Medical Research Council (MRC, United Kingdom)	MC_UU_00015/2	英国

• 引用: ジャーナル: J Biol Chem / 年: 2022; タイトル: Cryo-electron microscopy reveals how acetogenins inhibit mitochondrial respiratory complex I.; 著者: Daniel N Grba / James N Blaza / Hannah R Bridges / Ahmed-Noor A Agip / Zhan Yin / Masatoshi Murai / Hideto Miyoshi / Judy Hirst / ; 要旨: Mitochondrial complex I (NADH:ubiquinone oxidoreductase), a crucial enzyme in energy metabolism, captures the redox potential energy from NADH oxidation/ubiquinone reduction to create the proton motive force used to drive ATP synthesis in oxidative phosphorylation. High-resolution single-particle electron cryo-EM analyses have provided detailed structural knowledge of the catalytic machinery of complex I, but not of the molecular principles of its energy transduction mechanism. Although ubiquinone is considered to bind in a long channel at the interface of the membrane-embedded and hydrophilic domains, with channel residues likely involved in coupling substrate reduction to proton translocation, no structures with the channel fully occupied have yet been described. Here, we report the structure (determined by cryo-EM) of mouse complex I with a tight-binding natural product acetogenin inhibitor, which resembles the native substrate, bound along the full length of the expected ubiquinone-binding channel. Our structure reveals the mode of acetogenin binding and the molecular basis for structure-activity relationships within the acetogenin family. It also shows that acetogenins are such potent inhibitors because they are highly hydrophobic molecules that contain two specific hydrophilic moieties spaced to lock into two hydrophilic regions of the otherwise hydrophobic channel. The central hydrophilic section of the channel does not favor binding of the isoprenoid chain when the native substrate is fully bound but stabilizes the ubiquinone/ubiquinol headgroup as it transits to/from the active site. Therefore, the amphipathic nature of the channel supports both tight binding of the amphipathic inhibitor and rapid exchange of the ubiquinone/ubiquinol substrate and product.

履歴

登録	2021年9月22日	-
ヘッダ(付随情報) 公開	2022年4月6日	-
マップ公開	2022年4月6日	-
更新	2022年4月6日	-
現状	2022年4月6日	処理サイト: PDBe / 状態: 公開

マップ

• ファイル: ダウンロード / ファイル: emd_13611.map.gz / 形式: CCP4 / 大きさ: 347.6 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• 注釈: Globally sharpened map (postprocessed) in Relion
• ボクセルのサイズ: X=Y=Z: 1.043 Å

密度

表面レベル	登録者による: 0.035
最小 - 最大	-0.08404521 - 0.26540214
平均 (標準偏差)	-4.7450998e-05 (±0.00802398)

• 対称性: 空間群: 1

詳細

EMDB XML:

マップ形状	Axis order X Y Z Origin 0 0 0 サイズ 450 450 450 Spacing 450 450 450
セル	A=B=C: 469.35 Å α=β=γ: 90.0 °

添付データ

マスク #1

• ファイル: emd_13611_msk_1.map

追加マップ: Unfiltered consensus map from Relion

• ファイル: emd_13611_additional_1.map
• 注釈: Unfiltered consensus map from Relion

追加マップ: Locally sharpened map using LocalDeblur

• ファイル: emd_13611_additional_2.map
• 注釈: Locally sharpened map using LocalDeblur

ハーフマップ: Half map 1

• ファイル: emd_13611_half_map_1.map
• 注釈: Half map 1

ハーフマップ: Half map 2

• ファイル: emd_13611_half_map_2.map
• 注釈: Half map 2

試料の構成要素

全体 : Mitochondrial NADH:ubiquinone oxidoreductase from Mus musculus

• 全体: 名称: Mitochondrial NADH:ubiquinone oxidoreductase from Mus musculus

要素

• 複合体: Mitochondrial NADH:ubiquinone oxidoreductase from Mus musculus
  • タンパク質・ペプチド: NADH-ubiquinone oxidoreductase chain 3
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
  • タンパク質・ペプチド: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
  • タンパク質・ペプチド: NADH-ubiquinone oxidoreductase chain 1
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
  • タンパク質・ペプチド: NADH-ubiquinone oxidoreductase chain 6
  • タンパク質・ペプチド: NADH-ubiquinone oxidoreductase chain 4L
  • タンパク質・ペプチド: NADH-ubiquinone oxidoreductase chain 5
  • タンパク質・ペプチド: NADH-ubiquinone oxidoreductase chain 4
  • タンパク質・ペプチド: NADH-ubiquinone oxidoreductase chain 2
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
  • タンパク質・ペプチド: Acyl carrier protein, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 subunit C2
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial
• リガンド: IRON/SULFUR CLUSTER
• リガンド: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
• リガンド: FE2/S2 (INORGANIC) CLUSTER
• リガンド: FLAVIN MONONUCLEOTIDE
• リガンド: 1,2-Distearoyl-sn-glycerophosphoethanolamine
• リガンド: (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one
• リガンド: CARDIOLIPIN
• リガンド: ADENOSINE-5'-TRIPHOSPHATE
• リガンド: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
• リガンド: ZINC ION
• リガンド: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate

超分子 #1: Mitochondrial NADH:ubiquinone oxidoreductase from Mus musculus

• 超分子: 名称: Mitochondrial NADH:ubiquinone oxidoreductase from Mus musculus; タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#44; 詳細: Solubilised in DDM detergent. Native purification via anion exchange, with inhibitor acetogenin added, and size-exclusion chromatography.
• 由来(天然): 生物種: Mus musculus (ハツカネズミ)

分子 #1: NADH-ubiquinone oxidoreductase chain 3

• 分子: 名称: NADH-ubiquinone oxidoreductase chain 3 / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: house mouse (ハツカネズミ)
• 分子量: 理論値: 13.251785 KDa

配列

文字列:

(FME)NLYTVIFIN ILLSLTLILV AFWLPQMNLY SEKANPYECG FDPTSSARLP FSMKFFLVAI TFLLFDLEIA LLLPLP WAI QTIKTSTMMI MAFILVTILS LGLAYEWTQK GLEWTE

分子 #2: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial; タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 24.715912 KDa

配列

文字列:

MAALAAPGLL SVRILGLRTA QVQLRRVHQS VATEGPSPSP SPSLSSTQSA VSKAGAGAVV PKLSHLPRSR AEYVVTKLDD LINWARRSS LWPMTFGLAC CAVEMMHMAA PRYDMDRFGV VFRASPRQAD VMIVAGTLTN KMAPALRKVY DQMPEPRYVV S MGSCANGG GYYHYSYSVV RGCDRIVPVD IYVPGCPPTA EALLYGILQL QRKIKREQKL KIWYRR

分子 #3: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial; タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 30.191307 KDa

配列

文字列:

MAAAAARVWC RGLLGAASVG RGAGRPSVLW QHVRRESAAA DKRPTVRPRS DVTHKQLSAF GEYVAEILPK YVQQVQVSCL DELEICIHP DGVIPTLTFL RDHTNAQFKS LADLTAVDVP TRQNRFEIVY NLLSLRFNSR IRVKTYADEL TPIDSIVSVH I AANWYERE VWDMFGVFFF NHPDLRRILT DYGFEGHPFR KDFPLTGYVE LRYDDEVKRV VAEPVELAQE FRKFDLNSPW EA FPAYRQP PESLKLEAGD KKPETK

分子 #4: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial; タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 52.720602 KDa

配列

文字列:

MAALRALRCL RGVGAPVLRP GSGIRLPSQP SRGARQWQPD IEWAEQFSGA VMYPSKETAH WKPPPWNDVD ILKEKAVTNM TLNFGPQHP AAHGVLRLVL ELSGEMVRKC DPHIGLLH(2MR)G TEKLIEYKTY LQALPYFDRL DYVSMMCNEQ AYSIAVE KL LNIQPPPRAQ WIRVLFGEIT RILNHIMAVT THALDIGAMT PFFWMFEERE KMFEFYERVS GARMHAAYIR PGGVHQDL P LGLLDDIYEF SKNFSLRIDE VEEMLTNNRI WRNRTVDIGV VTAEDALNYG FSGVMLRGSG IQWDLRKTQP YDVYDQVEF DVPIGSRGDC YDRYLCRVEE MRQSLRIIEQ CLNKMPPGEI KVDDAKVSPP KRAEMKTSME SLIHHFKLYT EGYQVPPGAT YTAIEAPKG EFGVYLVSDG SSRPYRCKIK APGFAHLAGL DKMSKGHMLA DVVAIIGTQD IVFGEIDR

分子 #5: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 27.318336 KDa

配列

文字列:

MFSLALRARA TGLAAQWGRH ARNLHKTAVH NGAGGALFVH RDTPENNPDT PFDFTPENYK RIEAIVKNYP EGHQAAAVLP VLDLAQRQN GWLPISAMNK VAEVLQVPPM RVYEVATFYT MYNRKPVGKY HIQVCTTTPC MLRDSDSILE TLQRKLGIKV G ETTPDKLF TLIEVECLGA CVNAPMVQIN DNYYEDLTPK DIEEIIDELK AGKVPKPGPR SGRFCCEPAG GLTSLTEPPK GP GFGVQAG L

分子 #6: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial; タイプ: protein_or_peptide / ID: 6 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 50.904152 KDa

配列

文字列:

MLAARHFLGG LVPVRVSVRF SSGTTAPKKT SFGSLKDEDR IFTNLYGRHD WRLKGALRRG DWYKTKEILL KGPDWILGEM KTSGLRGRG GAGFPTGLKW SFMNKPSDGR PKYLVVNADE GEPGTCKDRE IMRHDPHKLV EGCLVGGRAM GARAAYIYIR G EFYNEASN LQVAIREAYE AGLIGKNACG SDYDFDVFVV RGAGAYICGE ETALIESIEG KQGKPRLKPP FPADVGVFGC PT TVANVET VAVSPTICRR GGTWFAGFGR ERNSGTKLFN ISGHVNHPCT VEEEMSVPLK ELIEKHAGGV TGGWDNLLAV IPG GSSTPL IPKSVCETVL MDFDALVQAQ TGLGTAAVIV MDRSTDIVKA IARLIEFYKH ESCGQCTPCR EGVDWMNKVM ARFV KGDAR PAEIDSLWEI SKQIEGHTIC ALGDGAAWPV QGLIRHFRPE LEDRMQRFAQ QHRAWQAAS

分子 #7: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

• 分子: 名称: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; タイプ: protein_or_peptide / ID: 7 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 79.866688 KDa

配列

文字列:

MLRIPIKRAL IGLSNSPKGY VRTTGTAASN LIEVFVDGQS VMVEPGTTVL QACEKVGMQI PRFCYHERLS VAGNCRMCLV EIEKAPKVV AACAMPVMKG WNILTNSEKS KKAREGVMEF LLANHPLDCP ICDQGGECDL QDQSMMFGSD RSRFLEGKRA V EDKNIGPL VKTIMTRCIQ CTRCIRFASE IAGVDDLGTT GRGNDMQVGT YIEKMFMSEL SGNVIDICPV GALTSKPYAF TA RPWETRK TESIDVMDAV GSNIVVSTRT GEVMRILPRM HEDINEEWIS DKTRFAYDGL KRQRLTEPMV RNEKGLLTYT SWE DALSRV AGMLQNFEGN AVAAIAGGLV DAEALVALKD LLNKVDSDNL CTEEIFPTEG AGTDLRSNYL LNTTIAGVEE ADVV LLVGT NPRFEAPLFN ARIRKSWLHN DLKVALIGSP VDLTYRYDHL GDSPKILQDI ASGRHSFCEV LKDAKKPMVV LGSSA LQRD DGAAILVAVS NMVQKIRVTT GVAAEWKVMN ILHRIASQVA ALDLGYKPGV EAIRKNPPKM LFLLGADGGC ITRQDL PKD CFIVYQGHHG DVGAPMADVI LPGAAYTEKS ATYVNTEGRA QQTKVAVTPP GLAREDWKII RALSEIAGIT LPYDTLD QV RNRLEEVSPN LVRYDDIEET NYFQQASELA KLVNQEVLAD PLVPPQLTIK DFYMTDSISR ASQTMAKCVK AVTEGAQA V EEPSIC

分子 #8: NADH-ubiquinone oxidoreductase chain 1

• 分子: 名称: NADH-ubiquinone oxidoreductase chain 1 / タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: house mouse (ハツカネズミ)
• 分子量: 理論値: 36.105027 KDa

配列

文字列:

(FME)FFINILTLL VPILIAMAFL TLVERKILGY MQLRKGPNIV GPYGILQPFA DAMKLFMKEP MRPLTTSMSL FIIAPT LSL TLALSLWVPL PMPHPLINLN LGILFILATS SLSVYSILWS GWASNSKYSL FGALRAVAQT ISYEVTMAII LLSVLLM NG SYSLQTLITT QEHMWLLLPA WPMAMMWFIS TLAETNRAPF DLTEGESELV SGFNVEYAAG PFALFFMAEY TNIILMNA L TTIIFLGPLY YINLPELYST NFMMEALLLS STFLWIRASY PRFRYDQLMH LLWKNFLPLT LALCMWHISL PIFTAGVPP YM

分子 #9: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial; タイプ: protein_or_peptide / ID: 9 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 24.068355 KDa

配列

文字列:

MYRLSSSMLP RALAQAMRTG HLNGQSLHSS AVAATYKYVN KKEQESEVDM KSATDNAARI LMWTELIRGL GMTLSYLFRE PATINYPFE KGPLSPRFRG EHALRRYPSG EERCIACKLC EAICPAQAIT IEAEPRADGS RRTTRYDIDM TKCIYCGFCQ E ACPVDAIV EGPNFEFSTE THEELLYNKE KLLNNGDKWE AEIAANIQAD YLYR

分子 #10: NADH-ubiquinone oxidoreductase chain 6

• 分子: 名称: NADH-ubiquinone oxidoreductase chain 6 / タイプ: protein_or_peptide / ID: 10 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 18.6561 KDa

配列

文字列:

(FME)NNYIFVLSS LFLVGCLGLA LKPSPIYGGL GLIVSGFVGC LMVLGFGGSF LGLMVFLIYL GGMLVVFGYT TAMATE EYP ETWGSNWLIL GFLVLGVIME VFLICVLNYY DEVGVINLDG LGDWLMYEVD DVGVMLEGGI GVAAMYSCAT WMMVVAG WS LFAGIFIIIE ITRD

分子 #11: NADH-ubiquinone oxidoreductase chain 4L

• 分子: 名称: NADH-ubiquinone oxidoreductase chain 4L / タイプ: protein_or_peptide / ID: 11 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: house mouse (ハツカネズミ)
• 分子量: 理論値: 10.637629 KDa

配列

文字列:

(FME)PSTFFNLTM AFSLSLLGTL MFRSHLMSTL LCLEGMVLSL FIMTSVTSLN SNSMSSMPIP ITILVFAACE AAVGLA LLV KVSNTYGTDY VQNLNLLQC

分子 #12: NADH-ubiquinone oxidoreductase chain 5

• 分子: 名称: NADH-ubiquinone oxidoreductase chain 5 / タイプ: protein_or_peptide / ID: 12 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 68.547297 KDa

配列

文字列:

(FME)NIFTTSILL IFILLLSPIL ISMSNLIKHI NFPLYTTTSI KFSFIISLLP LLMFFHNNME YMITTWHWVT MNSMEL KMS FKTDFFSILF TSVALFVTWS IMQFSSWYMH SDPNINRFIK YLTLFLITML ILTSANNMFQ LFIGWEGVGI MSFLLIG WW YGRTDANTAA LQAILYNRIG DIGFILAMVW FSLNMNSWEL QQIMFSNNND NLIPLMGLLI AATGKSAQFG LHPWLPSA M EGPTPVSALL HSSTMVVAGI FLLVRFHPLT TNNNFILTTM LCLGALTTLF TAICALTQND IKKIIAFSTS SQLGLMMVT LGMNQPHLAF LHICTHAFFK AMLFMCSGSI IHSLADEQDI RKMGNITKIM PFTSSCLVIG SLALTGMPFL TGFYSKDLII EAINTCNTN AWALLITLIA TSMTAMYSMR IIYFVTMTKP RFPPLISINE NDPDLMNPIK RLAFGSIFAG FVISYNIPPT S IPVLTMPW FLKTTALIIS VLGFLIALEL NNLTMKLSMN KANPYSSFST LLGFFPSIIH RITPMKSLNL SLKTSLTLLD LI WLEKTIP KSTSTLHTNM TTLTTNQKGL IKLYFMSFLI NIILIIILYS INLE

分子 #13: NADH-ubiquinone oxidoreductase chain 4

• 分子: 名称: NADH-ubiquinone oxidoreductase chain 4 / タイプ: protein_or_peptide / ID: 13 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: house mouse (ハツカネズミ)
• 分子量: 理論値: 51.943547 KDa

配列

文字列:

(FME)LKIILPSLM LLPLTWLSSP KKTWTNVTSY SFLISLTSLT LLWQTDENYK NFSNMFSSDP LSTPLIILTA WLLPLM LMA SQNHLKKDNN VLQKLYISML ISLQILLIMT FSATELIMFY ILFEATLIPT LIIITRWGNQ TERLNAGIYF LFYTLIG SI PLLIALILIQ NHVGTLNLMI LSFTTHTLDA SWSNNLLWLA CMMAFLIKMP LYGVHLWLPK AHVEAPIAGS MILAAILL K LGSYGMIRIS IILDPLTKYM AYPFILLSLW GMIMTSSICL RQTDLKSLIA YSSVSHMALV IASIMIQTPW SFMGATMLM IAHGLTSSLL FCLANSNYER IHSRTMIMAR GLQMVFPLMA TWWLMASLAN LALPPSINLM GELFITMSLF SWSNFTIILM GINIIITGM YSMYMIITTQ RGKLTNHMIN LQPSHTRELT LMALHMIPLI LLTTSPKLIT GLTM

分子 #14: NADH-ubiquinone oxidoreductase chain 2

• 分子: 名称: NADH-ubiquinone oxidoreductase chain 2 / タイプ: protein_or_peptide / ID: 14 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 38.80023 KDa

配列

文字列:

(FME)NPITLAIIY FTIFLGPVIT MSSTNLMLMW VGLEFSLLAI IPMLINKKNP RSTEAATKYF VTQATASMII LLAIVL NYK QLGTWMFQQQ TNGLILNMTL MALSMKLGLA PFHFWLPEVT QGIPLHMGLI LLTWQKIAPL SILIQIYPLL NSTIILM LA ITSIFMGAWG GLNQTQMRKI MAYSSIAHMG WMLAILPYNP SLTLLNLMIY IILTAPMFMA LMLNNSMTIN SISLLWNK T PAMLTMISLM LLSLGGLPPL TGFLPKWIII TELMKNNCLI MATLMAMMAL LNLFFYTRLI YSTSLTMFPT NNNSKMMTH QTKTKPNLMF STLAIMSTMT LPLAPQLIT

分子 #15: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mi...

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; タイプ: protein_or_peptide / ID: 15 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 40.657375 KDa

配列

文字列:

MALRLLRLVP ASAPARGLAA GAQRVGRIHT SVHCKLRYGL LAAILGDKTT KKLHEYSRVI TVDGNICSGK NKLAKEIAQQ LGMKHYPEA GIQYSSTTTG DGRPLDIEFS GSCSLEKFYD DPKSNDGNSY RLQSWLYASR LLQYADALEH LLSTGQGVVL E RSIYSDFV FLEAMYNQGY IRKQCVDHYN EIKRLTLPEY LPPHAVIYID VPVPEVQSRI QKKGDPHEMK VTSAYLQDIE NA YKKTFLP KMSEMCEVLV YDSWEAEDPT KVVEDIEYLK YNKGPWLKQD DWTFHYLRML VQDKTEVLNY TTIPVYLPEI TIG AHQGSR IYNSFRELPG RKYAPGYNAE VGDKWIWLK

分子 #16: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mit...

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial; タイプ: protein_or_peptide / ID: 16 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 42.588129 KDa

配列

文字列:

MAAAVRFRVV RALPMSRPAI TAAATSVFCG SSHRQLHHAV IPHGKGGRSS VSGVVATVFG ATGFLGRYVV NHLGRMGSQV IIPYRCDVY DIMHLRLMGD LGQLTFLEWD ARDKDSIRKA VQHSNVVINL IGREWETRNF DFEDVFVNIP RAIAQASKEA G VERFIHVS HLNASMKSSS KSLRSKAVGE KEVRSVFPEA IIIRPSDIFG REDRFLNHFA NYRWFLAVPL VSLGFKTVKQ PV YVADVSK GIVNATKDPD AVGKTFAFTG PNRYLLFHLV KYIFGMTHRT FIPYPLPLFV YSWIGKLFGL SPFEPWTTKD KVE RIHISD VMPTDLPGLE DLGVQPTPLE LKSIEVLRRH RTYRWLSSEI EETKPAKTVN Y

分子 #17: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial; タイプ: protein_or_peptide / ID: 17 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 19.814725 KDa

配列

文字列:

MAAVSISVSL RQAMLGRRAM ATAAVSVCRV PSRLLSTSTW KLADNQTRDT QLITVDEKLD ITTLTGVPEE HIKTRKVRIF VPARNNMQS GVNNTKKWKM EFDTRERWEN PLMGWASTAD PLSNMVLTFS AKEDAIAFAE KNGWSYDVEE KKVPKPKSKS Y GANFSWNK RTRVSTK

分子 #18: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial; タイプ: protein_or_peptide / ID: 18 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 13.041828 KDa

配列

文字列:

MAAVLTFRRL LTLPRAARGF GVQVSPSGEK ITHTGQVYDE KDYRRVRFVD RQKEVNENFA IDLIAQQPVN EVEHRIIACD GGGGALGHP KVYINLDKET KTGTCGYCGL QFKQHHH

分子 #19: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; タイプ: protein_or_peptide / ID: 19 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 10.932675 KDa

配列

文字列:

MAAAAASRAV GAKLGLREIR VHLCQRSPGS QGVRDFIVQR YVELKKAHPN LPILIRECSE VQPKLWARYA FGQEKTVSLN NLSADEVTR AMQNVLSGKA

分子 #20: Acyl carrier protein, mitochondrial

• 分子: 名称: Acyl carrier protein, mitochondrial / タイプ: protein_or_peptide / ID: 20 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 17.390289 KDa

配列

文字列:

MASRVLCACV RRLPAAFAPL PRLPTLALAR PLSTTLCPEG IRRRPGALQS ALALAQVPGT VTHLCRQYSD APPLTLDGIK DRVLYVLKL YDKIDPEKLS VNSHFMKDLG LDSLDQVEII MAMEDEFGFE IPDIDAEKLM CPQEIVDYIA DKKDVYE

分子 #21: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5; タイプ: protein_or_peptide / ID: 21 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 13.380719 KDa

配列

文字列:

MAGLLKKTTG LVGLAVCDTP HERLTILYTK TLDILKHFPK HAAYRKYTEQ ITNEKLDMVK AEPDVKKLEA LLQGGEVEEV ILQAEKELS LARKMLKWKP WEPLVEEPPA NQWKWPI

分子 #22: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6; タイプ: protein_or_peptide / ID: 22 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 15.311858 KDa

配列

文字列:

MAAAATGLRQ AAAAAASTSV KPIFSRDLNE AKRRVRELYR AWYREVPNTV HLMQLDITVK QGRDKVREMF MKNAHVTDPR VVDLLVIKG KMELQETIKV WKQRTHVMRF FHETETPRPK DFLSKFYMGH DP

分子 #23: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; タイプ: protein_or_peptide / ID: 23 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 20.025127 KDa

配列

文字列:

MPGIVELPTL EELKVEEVKV SSAVLKAAAH HYGAQCDKTN KEFMLCRWEE KDPRRCLKEG KLVNGCALNF FRQIKSHCAE PFTEYWTCL DYSNMQLFRH CRQQQAKFDQ CVLDKLGWVR PDLGQLSKVT KVKTDRPLPE NPYHSRARPE PNPVIEGDLK P AKHGTRFF FWTV

分子 #24: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11; タイプ: protein_or_peptide / ID: 24 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 15.130416 KDa

配列

文字列:

MAMVKRFFES YHEVPDGTQC HRKTYITTAL GGICGIIGSA YSVSLNPADS TLEAVARVGR YTFTAAAIGA MFGLTTCVSA QVREKPDDP LNYFIGGCAG GLTLGARTHS YGTAAMGCVY MGTAAALFKI GKLEGWELFP TPKV

分子 #25: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13; タイプ: protein_or_peptide / ID: 25 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 16.881588 KDa

配列

文字列:

MAASKVKQDM PPPGGYGPID YKRNLPRRGL SGYSMFAVGI GALIFGYWRM MRWNQERRRL LIEDLEARIA LMPLFQAEKD RRTLQILRE NLEEEAIIMK DVPNWKVGES VFHTTRWVPP LIGEMYGLRT KEEMSNANFG FTWYT

分子 #26: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; タイプ: protein_or_peptide / ID: 26 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 8.149524 KDa

配列

文字列:

MWFEILPGLA IMGVCLVIPG VSTAYIHKFT NGGKEKRVAR VQYQWYLMER DRRISGVNRY YVSKGLENID

分子 #27: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3; タイプ: protein_or_peptide / ID: 27 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 9.338867 KDa

配列

文字列:

MAGRISAFLK NAWAKEPVLV VSFSVWGLAI IMPMISPYTK YASMINKATP YNYPVPVRDD GNMPDVPSHP QDPLGPSLDW LKNL

分子 #28: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial; タイプ: protein_or_peptide / ID: 28 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 8.636023 KDa

配列

文字列:

MAPSVVLRSF SRLLAPARLP SCSSTRSKFY VREPVNAKPN WLAVGLSVGA SVFMWIYLIQ THNEDVLEYK RRNGLE

分子 #29: NADH dehydrogenase [ubiquinone] 1 subunit C2

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 subunit C2 / タイプ: protein_or_peptide / ID: 29 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: house mouse (ハツカネズミ)
• 分子量: 理論値: 14.185692 KDa

配列

文字列:

MMNGRPGHEP LKFLPDEARS LPPPKLNDPR LVYMGLLGYC TGLMDNMLRM RPVMRAGLHR QLLFVTSFVF AGYFYLKRQN YLYAVKDHD MFGYIKLHPE DFPEKEKKTY AEILEPFHPV R

分子 #30: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

• 分子: 名称: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5; タイプ: protein_or_peptide / ID: 30 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 12.675772 KDa

配列

文字列:

MPFLDIQKKL GISLDRHFMF LSAEQPYKNA ARCHAFEKEW IECAHGIGGT RAKKECKIEF DDFEECLLRY KTMRRMHDIK KQREKLMKE GKYTPPPHHS GREEPRP

分子 #31: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1; タイプ: protein_or_peptide / ID: 31 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 6.965109 KDa

配列

文字列:

MTLFQLLREH WVHILVPAGF VFGCYLDRKD DEKLTAFRNK SMLFQRELRP NEEVTWK

分子 #32: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mit...

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial; タイプ: protein_or_peptide / ID: 32 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 17.463727 KDa

配列

文字列:

MAARLLSLYG RCLSAAGAMR GLPAARVRWE SSRAVIAPSG VEKKRQREPT MQWQEDPEPE DENVYAKNPD FHGYDSDPVV DVWNMRAVF FFGFSIVLVF GTTFVAYVPD YRMQEWARRE AERLVKYREV NGLPIMESNY FDPSKIQLPE DD

分子 #33: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mito...

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial; タイプ: protein_or_peptide / ID: 33 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 21.742197 KDa

配列

文字列:

MAAMSLLQRA SVSALTALSC RRAGPRLGVG SFLTRSFPKT VAPVRHSGDH GKRLFVVKPS LYYDARFLRL MKFYLMLTGI PVIIGITLV NIFIGEAELA EIPEGYIPEH WEYYKHPISR WIARNFYDGP EKNYEKTLAI LQIESEKAEL RLKEQEVRRL M RARGDGPW YQFPTPEKEF IDHSPKATPD N

分子 #34: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6; タイプ: protein_or_peptide / ID: 34 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 15.540085 KDa

配列

文字列:

MSGYTPDEKL RLQQLRELRR RWLKDQELSP REPVLPPRRM WPLERFWDNF LRDGAVWKNM VFKAYRSSLF AVSHVLIPMW FVHYYVKYH MATKPYTIVS SKPRIFPGDT ILETGEVIPP MRDFPDQHH

分子 #35: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mito...

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial; タイプ: protein_or_peptide / ID: 35 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 11.982437 KDa

配列

文字列:

MSALTRLVPF GRVGGRLLRG CRARAAGDSG VRHAGGGVHI QPRYREFPQL TRSQVIQGEF LSSLMWFWIL WRFWHDSDAV LGHFSYPDP SQWTDEELGI PPDDED

分子 #36: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3; タイプ: protein_or_peptide / ID: 36 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 11.71424 KDa

配列

文字列:

MAAGHGHEHG HEHGHGHGKM ELPDYRQWKI EGTPLETVQK KLAARGLRDP WARNEAWRYM GGFAGNITFP SVILKGFKWG FAAFVVALG AEYFLDSQNG DKKHH

分子 #37: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; タイプ: protein_or_peptide / ID: 37 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 21.903828 KDa

配列

文字列:

MAAARAAALG VRWLQRTTRG VVPLEARRAF HMTKDMLPGS YPRTPEERAA AAKKYNMRVE DYEPYPDDGM GYGDYPMLPN RSQHERDPW YQWDHSELRM NWGEPIHWDL DMYIRNRVDT SPTPVSWDVM CKHLFGFVAF MVFMFWVGHV FPSYQPVGPK Q YPYNNLYL ERGGDPTKEP EPVVHYDI

分子 #38: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4; タイプ: protein_or_peptide / ID: 38 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 15.105287 KDa

配列

文字列:

MSGSKYKPAP LATLPSTLDP AEYDVSPETR RAQVERLSIR ARLKREYLLQ YNDPKRVSHI EDPALIRWTY ARSANIYPNF RPTPKNSLL GAVAGFGPLI FWYYVFKTDR DRKERLIQEG KLDRKFNISY

分子 #39: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; タイプ: protein_or_peptide / ID: 39 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 22.020123 KDa

配列

文字列:

MAFCAPPAYL THQQKVLRLY KRALRHLESW CIHRDKYRYF ACLMRARFEE HKNEKDMMRA TQLLREAEEE FWQNQHPQPY IFPDSPGGT SFERYECYKV PEWCLDYWHP SEKAMYPDYF SKREQWKKLR MESWDREVKQ LQEETSPDGI MTEALPPARR E GDLPPLWW HIVTRPRERP T

分子 #40: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7; タイプ: protein_or_peptide / ID: 40 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 16.360804 KDa

配列

文字列:

MGAHLTRRYL WDASVEPDPE KIPSFPPDLG FPERKERVMV ATQQEMMDAQ LTLQQRDYCA HYLIRLLKCK RDSFPNFLAC KHEQHDWDY CEHLDYVKRM KEFERERRLL QRKKRRALKE ARVAQGQGEG EVGPEVAL

分子 #41: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10; タイプ: protein_or_peptide / ID: 41 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 21.054832 KDa

配列

文字列:

MPDSWDKDVY PEPPSRTPAP SPQTSLPNPI TYLTKAYDLV VDWPVTLVRE FIERQHAKNR TYYYHRQYRR VPDITECKEG DVLCIYEAE MQWRRDFKVD QEIMNIIQER LKACQQREGE NYQQNCAKEL EQFTKVTKAY QDRYLDLGAY YSARKCLAKQ K QRMLEERK AARQEAAA

分子 #42: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12; タイプ: protein_or_peptide / ID: 42 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 17.154453 KDa

配列

文字列:

(AME)ELVEVLKRG VQQVTGHGGL RGLLRVFFRA NDIRIGTLVG EDKYGNKYYE DNKQFFGRHR WVIYTTEMNG KNTFWD VDG SMVPPEWHRW LHCMTDDPPT TNPPTARKFI WTNHKFNVSA TPEQYVPYST TRKKIHEWVP PSTPYK

分子 #43: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7; タイプ: protein_or_peptide / ID: 43 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 12.637629 KDa

配列

文字列:

M(AYA)SATRVIQK LRNWASGQDL QAKLQLRYQE IAKRTQPPPK LPVGPSHKLS NNYYCTRDGR REVVPPSIIM SSQKAL VSG KAAESSAMAA TEKKAVTPAP PMKRWELSKD QPYL

分子 #44: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial; タイプ: protein_or_peptide / ID: 44 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mouse (ネズミ)
• 分子量: 理論値: 11.833504 KDa

配列

文字列:

MAVSLLLRGG RIRALKAVLL EARVFPGELV SVVRLSTESE KSAKEKELHP KTQSVLKEPE PTDTTTYKNL QHHDYNTYTF LDLNLDLSK FRLPQPSSGR ESPRH

分子 #45: IRON/SULFUR CLUSTER

• 分子: 名称: IRON/SULFUR CLUSTER / タイプ: ligand / ID: 45 / コピー数: 6 / 式: SF4
• 分子量: 理論値: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / 一硫化鉄

分子 #46: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

• 分子: 名称: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / タイプ: ligand / ID: 46 / コピー数: 4 / 式: PC1
• 分子量: 理論値: 790.145 Da

Chemical component information

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質*YM

分子 #47: FE2/S2 (INORGANIC) CLUSTER

• 分子: 名称: FE2/S2 (INORGANIC) CLUSTER / タイプ: ligand / ID: 47 / コピー数: 2 / 式: FES
• 分子量: 理論値: 175.82 Da

Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

分子 #48: FLAVIN MONONUCLEOTIDE

• 分子: 名称: FLAVIN MONONUCLEOTIDE / タイプ: ligand / ID: 48 / コピー数: 1 / 式: FMN
• 分子量: 理論値: 456.344 Da

Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE / FMN

分子 #49: 1,2-Distearoyl-sn-glycerophosphoethanolamine

• 分子: 名称: 1,2-Distearoyl-sn-glycerophosphoethanolamine / タイプ: ligand / ID: 49 / コピー数: 9 / 式: 3PE
• 分子量: 理論値: 748.065 Da

Chemical component information

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / DSPE / リン脂質*YM

分子 #50: (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-...

• 分子: 名称: (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one; タイプ: ligand / ID: 50 / コピー数: 1 / 式: 88I
• 分子量: 理論値: 608.932 Da

Chemical component information

ChemComp-88I:
(3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one

分子 #51: CARDIOLIPIN

• 分子: 名称: CARDIOLIPIN / タイプ: ligand / ID: 51 / コピー数: 7 / 式: CDL
• 分子量: 理論値: 1.464043 KDa

Chemical component information

ChemComp-CDL:
CARDIOLIPIN / リン脂質*YM

分子 #52: ADENOSINE-5'-TRIPHOSPHATE

• 分子: 名称: ADENOSINE-5'-TRIPHOSPHATE / タイプ: ligand / ID: 52 / コピー数: 1 / 式: ATP
• 分子量: 理論値: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子*YM

分子 #53: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

• 分子: 名称: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE; タイプ: ligand / ID: 53 / コピー数: 1 / 式: NDP
• 分子量: 理論値: 745.421 Da

Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / NADPH

分子 #54: ZINC ION

• 分子: 名称: ZINC ION / タイプ: ligand / ID: 54 / コピー数: 1 / 式: ZN
• 分子量: 理論値: 65.409 Da

分子 #55: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butan...

• 分子: 名称: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate; タイプ: ligand / ID: 55 / コピー数: 2 / 式: EHZ
• 分子量: 理論値: 584.703 Da

Chemical component information

ChemComp-EHZ:
~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate

実験情報

構造解析

• 手法: クライオ電子顕微鏡法
• 解析: 単粒子再構成法
• 試料の集合状態: particle

試料調製

緩衝液

pH: 7.55
構成要素:

濃度	名称
20.0 mM	Tris-HCl
200.0 mM	NaCl
0.02 w/v (%)	n-Dodecyl-B-D-Maltoside

詳細: pH 7.55 at 25 degrees Celsius

• グリッド: モデル: UltrAuFoil R0.6/1 / 材質: GOLD / メッシュ: 300 / 支持フィルム - 材質: GOLD / 支持フィルム - トポロジー: HOLEY / 支持フィルム - Film thickness: 50.0 nm / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 雰囲気: AIR; 詳細: 20 mA glow discharge. Followed by a 2-day PEGylation with a PEG-thiol reagent.
• 凍結: 凍結剤: ETHANE / チャンバー内湿度: 95 % / チャンバー内温度: 277.15 K / 装置: FEI VITROBOT MARK III

電子顕微鏡法

• 顕微鏡: FEI TITAN KRIOS
• 特殊光学系: エネルギーフィルター - スリット幅: 20 eV
• 撮影: フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k); 検出モード: COUNTING / 撮影したグリッド数: 1 / 実像数: 1286 / 平均露光時間: 10.0 sec. / 平均電子線量: 50.0 e/Ų
• 電子線: 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
• 電子光学系: 照射モード: SPOT SCAN / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス（公称値）: 2.7 µm / 最小 デフォーカス（公称値）: 1.5 µm / 倍率（公称値）: 130000
• 試料ステージ: 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER
• 実験機器: モデル: Titan Krios / 画像提供: FEI Company

画像解析

• 粒子像選択: 選択した数: 114209
• CTF補正: ソフトウェア - 名称: Gctf
• 初期モデル: モデルのタイプ: EMDB MAP; 詳細: 60-angstrom low-pass-filtered in-house map from previous work
• 最終 再構成: 使用したクラス数: 1 / 想定した対称性 - 点群: C₁ (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 3.4 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION (ver. 3.1) / 詳細: Generated with a mask in RELION / 使用した粒子像数: 15754
• 初期 角度割当: タイプ: PROJECTION MATCHING / ソフトウェア - 名称: RELION (ver. 3.1); 詳細: Initial 2D classification to remove non-protein particles
• 最終 角度割当: タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION (ver. 3.1); 詳細: RELION solvent-flattened 3D refinement with a PDB-generated mask
• 最終 3次元分類: クラス数: 2 / ソフトウェア - 名称: RELION (ver. 3.1); 詳細: Used a mask classification without alignment on a small region to isolated a compound-bound state.

原子モデル構築 1

初期モデル

PDB ID:

6zr2

• 精密化: 空間: REAL

得られたモデル

PDB-7psa:
The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms