+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-34519 | |||||||||
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タイトル | Hsp90-AhR-p23-XAP2 complex | |||||||||
マップデータ | ||||||||||
試料 |
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キーワード | Hsp90 / AhR / PASB doamin / complex / p23 / XAP2 / CYTOSOLIC PROTEIN | |||||||||
機能・相同性 | 機能・相同性情報 circumferential growth involved in left ventricle morphogenesis / negative regulation of calcium ion transmembrane transport / cellular response to 3-methylcholanthrene / regulation of heart growth / GAF domain binding / ESR-mediated signaling / cytosolic aryl hydrocarbon receptor complex / glomerulus morphogenesis / DDX58/IFIH1-mediated induction of interferon-alpha/beta / The NLRP3 inflammasome ...circumferential growth involved in left ventricle morphogenesis / negative regulation of calcium ion transmembrane transport / cellular response to 3-methylcholanthrene / regulation of heart growth / GAF domain binding / ESR-mediated signaling / cytosolic aryl hydrocarbon receptor complex / glomerulus morphogenesis / DDX58/IFIH1-mediated induction of interferon-alpha/beta / The NLRP3 inflammasome / gland development / HSF1-dependent transactivation / reactive oxygen species biosynthetic process / Phase I - Functionalization of compounds / Xenobiotics / Aryl hydrocarbon receptor signalling / HSF1 activation / kidney morphogenesis / RHOBTB2 GTPase cycle / lung saccule development / ooplasm / Sema3A PAK dependent Axon repulsion / omega-hydroxylase P450 pathway / prostaglandin-E synthase / prostaglandin-E synthase activity / arachidonate omega-hydroxylase activity / positive regulation of growth rate / Attenuation phase / lymphocyte homeostasis / regulation of adaptive immune response / receptor ligand inhibitor activity / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / negative regulation of complement-dependent cytotoxicity / cellular response to 2,3,7,8-tetrachlorodibenzodioxine / telomerase activity / Endogenous sterols / nuclear aryl hydrocarbon receptor complex / HSP90-CDC37 chaperone complex / Synthesis of Prostaglandins (PG) and Thromboxanes (TX) / negative regulation of cyclic-nucleotide phosphodiesterase activity / nuclear receptor-mediated glucocorticoid signaling pathway / sperm head plasma membrane / cardiac left ventricle morphogenesis / The role of GTSE1 in G2/M progression after G2 checkpoint / negative regulation of proteasomal protein catabolic process / negative regulation of osteoblast proliferation / prostate gland development / reproductive structure development / cellular response to toxic substance / aryl hydrocarbon receptor complex / negative regulation of T cell mediated immune response to tumor cell / Regulation of actin dynamics for phagocytic cup formation / dynein axonemal particle / histone methyltransferase binding / B-1 B cell homeostasis / Estrogen-dependent gene expression / regulation of protein kinase A signaling / COP9 signalosome / post-embryonic hemopoiesis / E-box binding / negative regulation of DNA biosynthetic process / protein targeting to mitochondrion / positive regulation of protein localization to cell surface / ATP-dependent protein binding / vasculature development / camera-type eye development / negative regulation of systemic arterial blood pressure / telomerase holoenzyme complex / blood circulation / glycogen biosynthetic process / protein folding chaperone complex / negative regulation of protein metabolic process / blood vessel morphogenesis / prostaglandin biosynthetic process / protein maturation by protein folding / dATP binding / sulfonylurea receptor binding / CTP binding / UTP binding / skin development / negative regulation of vasoconstriction / telomerase holoenzyme complex assembly / branching involved in blood vessel morphogenesis / immune system process / prostaglandin metabolic process / negative regulation of osteoblast differentiation / heterocyclic compound binding / TPR domain binding / blood vessel development / positive regulation of transforming growth factor beta receptor signaling pathway / T cell homeostasis / dendritic growth cone / positive regulation of phosphorylation / aryl hydrocarbon receptor binding / B cell homeostasis / : / regulation of protein ubiquitination / chaperone cofactor-dependent protein refolding / telomere maintenance via telomerase / positive regulation of cell size 類似検索 - 分子機能 | |||||||||
生物種 | Mus musculus (ハツカネズミ) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.2 Å | |||||||||
データ登録者 | Wen ZL / Zhai YJ / Zhu Y / Sun F | |||||||||
資金援助 | 1件
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引用 | ジャーナル: Structure / 年: 2023 タイトル: Cryo-EM structure of the cytosolic AhR complex. 著者: Zuoling Wen / Yuebin Zhang / Beirong Zhang / Yumo Hang / Li Xu / Yangsheng Chen / Qunhui Xie / Qun Zhao / Lihua Zhang / Guohui Li / Bin Zhao / Fei Sun / Yujia Zhai / Yun Zhu / 要旨: Aryl hydrocarbon receptor (AhR) is an important ligand-activated transcription factor involved in the regulation of various important physiological functions. Here, we report the cryo-EM structures ...Aryl hydrocarbon receptor (AhR) is an important ligand-activated transcription factor involved in the regulation of various important physiological functions. Here, we report the cryo-EM structures of the Hsp90-AhR-p23 complex with or without bound XAP2, where the structure of the mouse AhR PAS-B domain is resolved. A highly conserved bridge motif of AhR is responsible for the interaction with the Hsp90 dimeric lumen. The ligand-free AhR PAS-B domain is attached to the Hsp90 dimer and is stabilized in the complex with bound XAP2. In addition, the DE-loop and a group of conserved pocket inner residues in the AhR PAS-B domain are found to be important for ligand binding. These results reveal the structural basis of the biological functions of AhR. Moreover, the protein purification method presented here allows the isolation of stable mouse AhR protein, which could be used to develop high-sensitivity biosensors for environmental pollutant detection. | |||||||||
履歴 |
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-構造の表示
添付画像 |
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-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_34519.map.gz | 6.5 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-34519-v30.xml emd-34519.xml | 20.6 KB 20.6 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_34519_fsc.xml | 9.1 KB | 表示 | FSCデータファイル |
画像 | emd_34519.png | 124.6 KB | ||
Filedesc metadata | emd-34519.cif.gz | 6.6 KB | ||
その他 | emd_34519_half_map_1.map.gz emd_34519_half_map_2.map.gz | 49.6 MB 49.6 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-34519 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-34519 | HTTPS FTP |
-関連構造データ
関連構造データ | 8h77MC 7y04C M: このマップから作成された原子モデル C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_34519.map.gz / 形式: CCP4 / 大きさ: 64 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.07 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-ハーフマップ: #2
ファイル | emd_34519_half_map_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_34519_half_map_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
+全体 : mouse Hsp90-AhR-p23-XAP2 complex
+超分子 #1: mouse Hsp90-AhR-p23-XAP2 complex
+超分子 #2: Heat shock protein HSP 90-beta
+超分子 #3: Prostaglandin E synthase 3
+超分子 #4: Aryl hydrocarbon receptor
+超分子 #5: AH receptor-interacting protein
+分子 #1: Heat shock protein HSP 90-beta
+分子 #2: Prostaglandin E synthase 3
+分子 #3: Aryl hydrocarbon receptor
+分子 #4: AH receptor-interacting protein
+分子 #5: ADENOSINE-5'-DIPHOSPHATE
+分子 #6: BERYLLIUM TRIFLUORIDE ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.5 |
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凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 60.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 1.8 µm / 最小 デフォーカス(公称値): 1.2 µm |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |