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- EMDB-0078: Wide Pick Filament from Pick's disease brain -

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Basic information

Database: EMDB / ID: 0078
TitleWide Pick Filament from Pick's disease brain
Map data
Samplemicrotubule-associated protein tauTau protein
  • Wide Pick Filament from Pick's disease brain
Methodhelical reconstruction / cryo EM / 8 Å resolution
AuthorsFalcon B / Zhang W / Murzin AG / Murshudov G / Garringer HJ / Vidal R / Crowther RA / Ghetti B / Scheres SHW / Goedert M
CitationJournal: Nature / Year: 2018
Title: Structures of filaments from Pick's disease reveal a novel tau protein fold.
Authors: Benjamin Falcon / Wenjuan Zhang / Alexey G Murzin / Garib Murshudov / Holly J Garringer / Ruben Vidal / R Anthony Crowther / Bernardino Ghetti / Sjors H W Scheres / Michel Goedert
DateDeposition: Jun 26, 2018 / Header (metadata) release: Aug 22, 2018 / Map release: Sep 12, 2018 / Last update: Sep 19, 2018

Structure visualization

  • Surface view with section colored by density value
  • Surface level: 0.17
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by cylindrical radius
  • Surface level: 0.17
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
Supplemental images

Downloads & links


Fileemd_0078.map.gz (map file in CCP4 format, 23329 KB)
Projections & slices

Image control

AxesZ (Sec.)Y (Row.)X (Col.)
180 pix
3.45 Å/pix.
= 621. Å
180 pix
3.45 Å/pix.
= 621. Å
180 pix
3.45 Å/pix.
= 621. Å



Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 3.45 Å
Contour Level:0.17 (by author), 0.17 (movie #1):
Minimum - Maximum-0.1330608 - 0.51759523
Average (Standard dev.)-0.0033756108 (0.033847462)


Space Group Number1
Map Geometry
Axis orderXYZ
CellA=B=C: 621.0 Å
α=β=γ: 90.0 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z3.453.453.45
M x/y/z180180180
origin x/y/z0.0000.0000.000
length x/y/z621.000621.000621.000
start NX/NY/NZ
MAP C/R/S123
start NC/NR/NS000
D min/max/mean-0.1330.518-0.003

Supplemental data

Mask #1

Projections & Slices
Slices (1/2)
Density Histograms

Sample components

Entire microtubule-associated protein tau

EntireName: microtubule-associated protein tau / Number of components: 2

Component #1: cellular-component, microtubule-associated protein tau

Cellular-componentName: microtubule-associated protein tauTau protein / Recombinant expression: No

Component #2: protein, Wide Pick Filament from Pick's disease brain

ProteinName: Wide Pick Filament from Pick's disease brain / Recombinant expression: No

Experimental details

Sample preparation

SpecimenSpecimen state: tissue / Method: cryo EM
Helical parametersAxial symmetry: C2 (2 fold cyclic) / Hand: RIGHT HANDED / Delta z: 4.7 Å / Delta phi: -0.6 deg.
Sample solutionSpecimen conc.: 0.5 mg/ml / pH: 7.4
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 277 K / Humidity: 100 %

Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 1.06 e/Å2 / Illumination mode: FLOOD BEAM
LensCs: 2.7 mm / Imaging mode: BRIGHT FIELD / Defocus: 1700.0 - 2800.0 nm / Energy filter: GIF Quantum LS
Specimen HolderModel: OTHER
CameraDetector: GATAN K2 QUANTUM (4k x 4k)

Image processing

ProcessingMethod: helical reconstruction
3D reconstructionResolution: 8 Å / Resolution method: FSC 0.143 CUT-OFF
FSC plot
(resolution estimation)

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