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- EMDB-7489: The tether and tether head complex and I1 dynein complex region e... -

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Entry
Database: EMDB / ID: EMD-7489
TitleThe tether and tether head complex and I1 dynein complex region extracted from the cryo-electron tomography and subtomographic average (1150 axonemal repeats) of isolated Tetrahymena FAP43KO mutant cilia
Map dataThe tether and tether head complex and I1 dynein complex region extracted from the cryo-electron tomography and subtomographic average (1150 axonemal repeats) of isolated Tetrahymena FAP43KO mutant cilia
Sample
  • Organelle or cellular component: The tether and tether head complex and I1 dynein complex region extracted from the cryo-electron tomography and subtomographic average (1150 axonemal repeats) of isolated Tetrahymena FAP43KO mutant cilia, the tether and tether head complex is missing in this mutant
Biological speciesTetrahymena thermophila (eukaryote)
Methodelectron tomography / cryo EM / Resolution: 39.0 Å
AuthorsFu G / Wang Q / Phan N / Urbanska P / Joachimiak E / Lin J / Wloga D / Nicastro D
CitationJournal: Mol Biol Cell / Year: 2018
Title: The I1 dynein-associated tether and tether head complex is a conserved regulator of ciliary motility.
Authors: Gang Fu / Qian Wang / Nhan Phan / Paulina Urbanska / Ewa Joachimiak / Jianfeng Lin / Dorota Wloga / Daniela Nicastro /
Abstract: Motile cilia are essential for propelling cells and moving fluids across tissues. The activity of axonemal dynein motors must be precisely coordinated to generate ciliary motility, but their ...Motile cilia are essential for propelling cells and moving fluids across tissues. The activity of axonemal dynein motors must be precisely coordinated to generate ciliary motility, but their regulatory mechanisms are not well understood. The tether and tether head (T/TH) complex was hypothesized to provide mechanical feedback during ciliary beating because it links the motor domains of the regulatory I1 dynein to the ciliary doublet microtubule. Combining genetic and biochemical approaches with cryoelectron tomography, we identified FAP44 and FAP43 (plus the algae-specific, FAP43-redundant FAP244) as T/TH components. WT-mutant comparisons revealed that the heterodimeric T/TH complex is required for the positional stability of the I1 dynein motor domains, stable anchoring of CK1 kinase, and proper phosphorylation of the regulatory IC138-subunit. T/TH also interacts with inner dynein arm d and radial spoke 3, another important motility regulator. The T/TH complex is a conserved regulator of I1 dynein and plays an important role in the signaling pathway that is critical for normal ciliary motility.
History
DepositionMar 1, 2018-
Header (metadata) releaseMar 14, 2018-
Map releaseMar 21, 2018-
UpdateMay 16, 2018-
Current statusMay 16, 2018Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 126
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by cylindrical radius
  • Surface level: 126
  • Imaged by UCSF Chimera
  • Download
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Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_7489.png

Downloads & links

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Map

FileDownload / File: emd_7489.map.gz / Format: CCP4 / Size: 516.6 KB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationThe tether and tether head complex and I1 dynein complex region extracted from the cryo-electron tomography and subtomographic average (1150 axonemal repeats) of isolated Tetrahymena FAP43KO mutant cilia
Voxel sizeX=Y=Z: 10.77 Å
Density
Contour LevelMovie #1: 126
Minimum - Maximum94.945660000000004 - 171.766130000000004
Average (Standard dev.)124.384699999999995 (±9.357412)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-30-39-32
Dimensions406055
Spacing604055
CellA: 646.2 Å / B: 430.80002 Å / C: 592.35004 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z10.7710.7710.77
M x/y/z604055
origin x/y/z0.0000.0000.000
length x/y/z646.200430.800592.350
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS-39-30-32
NC/NR/NS604055
D min/max/mean94.946171.766124.385

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Supplemental data

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Sample components

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Entire : The tether and tether head complex and I1 dynein complex region e...

EntireName: The tether and tether head complex and I1 dynein complex region extracted from the cryo-electron tomography and subtomographic average (1150 axonemal repeats) of isolated Tetrahymena FAP43KO ...Name: The tether and tether head complex and I1 dynein complex region extracted from the cryo-electron tomography and subtomographic average (1150 axonemal repeats) of isolated Tetrahymena FAP43KO mutant cilia, the tether and tether head complex is missing in this mutant
Components
  • Organelle or cellular component: The tether and tether head complex and I1 dynein complex region extracted from the cryo-electron tomography and subtomographic average (1150 axonemal repeats) of isolated Tetrahymena FAP43KO mutant cilia, the tether and tether head complex is missing in this mutant

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Supramolecule #1: The tether and tether head complex and I1 dynein complex region e...

SupramoleculeName: The tether and tether head complex and I1 dynein complex region extracted from the cryo-electron tomography and subtomographic average (1150 axonemal repeats) of isolated Tetrahymena FAP43KO ...Name: The tether and tether head complex and I1 dynein complex region extracted from the cryo-electron tomography and subtomographic average (1150 axonemal repeats) of isolated Tetrahymena FAP43KO mutant cilia, the tether and tether head complex is missing in this mutant
type: organelle_or_cellular_component / ID: 1 / Parent: 0
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: FAP43KO mutant

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Experimental details

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Structure determination

Methodcryo EM
Processingelectron tomography
Aggregation statetissue

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Sample preparation

BufferpH: 7.4
Component:
ConcentrationFormula
30.0 mMHEPES
25.0 mMKCl
5.0 mMMgSO4
1.0 mMEGTA
0.1 mMEDTAEthylenediaminetetraacetic acid

Details: Solutions were freshly made before use.
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 101.325 kPa
VitrificationCryogen name: ETHANE / Chamber temperature: 100 K / Instrument: HOMEMADE PLUNGER
Details: back-side blotting for 1.5-2.5 seconds before plunging.
SectioningOther: NO SECTIONING
Fiducial markerManufacturer: Sigma-Aldrich / Diameter: 10 nm

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Electron microscopy

MicroscopeFEI TECNAI F30
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 8.0 µm / Nominal defocus min: 6.0 µm / Nominal magnification: 13500
Specialist opticsEnergy filter - Name: GIF 2000 / Energy filter - Lower energy threshold: 0 eV / Energy filter - Upper energy threshold: 20 eV
Sample stageSpecimen holder model: GATAN LIQUID NITROGEN / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN ULTRASCAN 1000 (2k x 2k) / Average electron dose: 1.5 e/Å2
Experimental equipment
Model: Tecnai F30 / Image courtesy: FEI Company

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Image processing

Final reconstructionAlgorithm: BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 39.0 Å / Resolution method: FSC 0.5 CUT-OFF / Software - Name: IMOD
Software - details: fiducial alignment and weighted backprojection
Number images used: 600

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