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- EMDB-5205: Structure of microtubule decorated with PRC1 -

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Basic information

Database: EMDB / ID: 5205
TitleStructure of microtubule decorated with PRC1
Map dataThis is an image of section of microtubule decorated with PRC1
Methodhelical reconstruction, at 18 Å resolution
AuthorsSubramanian R / Wilson-Kubalek EM / Arthur CP / Bick MJ / Campbell EA / Darst SA / Milligan RA / Kapoor TM
CitationCell, 2010, 142, 433-443

Cell, 2010, 142, 433-443 Yorodumi Papers
Insights into antiparallel microtubule crosslinking by PRC1, a conserved nonmotor microtubule binding protein.
Radhika Subramanian / Elizabeth M Wilson-Kubalek / Christopher P Arthur / Matthew J Bick / Elizabeth A Campbell / Seth A Darst / Ronald A Milligan / Tarun M Kapoor

DateDeposition: Jun 30, 2010 / Header (metadata) release: Sep 10, 2010 / Map release: Sep 10, 2010 / Last update: Oct 19, 2010

Structure visualization

  • Surface view with section colored by density value
  • Surface level: 14
  • Imaged by UCSF CHIMERA
  • Download
  • Surface view colored by cylindrical radius
  • Surface level: 14
  • Imaged by UCSF CHIMERA
  • Download
3D viewer

View / / Stereo:
Slabnear <=> far

fix: /
Orientation Rotation
Misc. /
Supplemental images

Downloads & links


Fileemd_5205.map.gz (map file in CCP4 format, 11766 KB)
Projections & slices

Image control

AxesZ (Sec.)Y (Row.)X (Col.)
103 pix
5.35 Å/pix.
= 550.535 Å
171 pix
5.35 Å/pix.
= 913.995 Å
171 pix
5.35 Å/pix.
= 913.995 Å



Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider package.

Voxel size
EMDB info.5.3455.3455.345
CCP4 map header5.3455.3455.345
EM Navigator Movie #13.473.473.47
Contour Level:2.5 (by author), 14 (movie #1):
Minimum - Maximum-32 - 50
Average (Standard dev.)0.847337 (10.7397)


Space Group Number1
Map Geometry
Axis orderXYZ
CellA: 913.995 Å / B: 913.995 Å / C: 550.535 Å
α=β=γ: 90 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z5.3455.3455.345
M x/y/z171171103
origin x/y/z0.0000.0000.000
length x/y/z913.995913.995550.535
start NX/NY/NZ-99-99-99
MAP C/R/S123
start NC/NR/NS000
D min/max/mean-32.00050.0000.847

Supplemental data

Sample components

Entire PRC1

EntireName: PRC1 / Details: Decorated microtubules / Number of components: 2

Component #1: protein, Microtubule

ProteinName: Microtubule / a.k.a: PRC1 / Recombinant expression: Yes
SourceSpecies: Unidentified

Experimental details

Sample preparation

Specimen statefilament
Helical parametersHand: LEFT HANDED
VitrificationInstrument: NONE / Cryogen name: ETHANE

Electron microscopy imaging

Experimental equipment
Model: Tecnai F20 / Image courtesy: FEI Company
ImagingMicroscope: FEI TECNAI F20 / Date: Feb 2, 2010
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 120 kV / Electron dose: 10 e/Å2 / Electron beam tilt params: 0 / Illumination mode: FLOOD BEAM
LensMagnification: 35000 X (nominal) / Cs: 2 mm / Imaging mode: BRIGHT FIELD / Defocus: 900 - 2000 nm
Specimen HolderHolder: Eucentric / Model: GATAN LIQUID NITROGEN / Temperature: 93 K ( 93 - 93 K)
CameraDetector: GENERIC GATAN

Image acquisition

Image acquisitionNumber of digital images: 10

Image processing

ProcessingMethod: helical reconstruction
3D reconstructionAlgorithm: Fourier Bessel inversion / Software: PHOELIX / CTF correction: CTF correction of each micrograph / Resolution: 18 Å / Resolution method: Coherent phase determination

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