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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-1048 | |||||||||
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Title | Three-dimensional structure of bacteriophage T4 baseplate. | |||||||||
![]() | map contains the baseplate and the proximal part of the tail tube | |||||||||
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Function / homology | ![]() symbiont entry into host cell via disruption of host cell wall peptidoglycan / virus tail, baseplate / viral tail assembly / virus tail, fiber / symbiont entry into host cell via disruption of host cell envelope / virus tail / symbiont entry into host / viral release from host cell / peptidoglycan catabolic process / cell wall macromolecule catabolic process ...symbiont entry into host cell via disruption of host cell wall peptidoglycan / virus tail, baseplate / viral tail assembly / virus tail, fiber / symbiont entry into host cell via disruption of host cell envelope / virus tail / symbiont entry into host / viral release from host cell / peptidoglycan catabolic process / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / killing of cells of another organism / entry receptor-mediated virion attachment to host cell / defense response to bacterium / symbiont entry into host cell / identical protein binding / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 12.0 Å | |||||||||
![]() | Kostyuchenko VA / Leiman PG / Chipman PR / Kanamaru S / vanRaaij MJ / Arisaka F / Mesyanzhinov VV / Rossmann MG | |||||||||
![]() | ![]() Title: Three-dimensional structure of bacteriophage T4 baseplate. Authors: Victor A Kostyuchenko / Petr G Leiman / Paul R Chipman / Shuji Kanamaru / Mark J van Raaij / Fumio Arisaka / Vadim V Mesyanzhinov / Michael G Rossmann / ![]() Abstract: The baseplate of bacteriophage T4 is a multiprotein molecular machine that controls host cell recognition, attachment, tail sheath contraction and viral DNA ejection. We report here the three- ...The baseplate of bacteriophage T4 is a multiprotein molecular machine that controls host cell recognition, attachment, tail sheath contraction and viral DNA ejection. We report here the three-dimensional structure of the baseplate-tail tube complex determined to a resolution of 12 A by cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the center of the dome is a needle-like structure that was previously identified as a cell puncturing device. We have identified the locations of six proteins with known atomic structures, and established the position and shape of several other baseplate proteins. The baseplate structure suggests a mechanism of baseplate triggering and structural transition during the initial stages of T4 infection. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 3.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14 KB 14 KB | Display Display | ![]() |
Images | ![]() | 53.1 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1pdfMC ![]() 1pdiMC ![]() 1pdjMC ![]() 1pdlMC ![]() 1pdmMC ![]() 1pdpMC ![]() 2fl8M ![]() 3h3wM M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | map contains the baseplate and the proximal part of the tail tube | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 2.97872 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Bacteriophage T4 baseplate-tail tube assembly
Entire | Name: Bacteriophage T4 baseplate-tail tube assembly |
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Components |
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-Supramolecule #1000: Bacteriophage T4 baseplate-tail tube assembly
Supramolecule | Name: Bacteriophage T4 baseplate-tail tube assembly / type: sample / ID: 1000 / Oligomeric state: the complex has sixfold symmetry / Number unique components: 1 |
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-Macromolecule #1: baseplate-tail tube complex
Macromolecule | Name: baseplate-tail tube complex / type: protein_or_peptide / ID: 1 / Recombinant expression: Yes |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: Escherichia coli BE |