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- EMDB-0057: Cryo-EM structure of a partial yeast 48S preinitiation complex in... -

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Basic information

Entry
Database: EMDB / ID: EMD-0057
TitleCryo-EM structure of a partial yeast 48S preinitiation complex in its open conformation.
Map dataFor optimal visualization of eIF2, and of eIF3 at the 40S subunit interface, gauss-filter the map by 1.34 and display it at 0.04 or 0.03 contour level, respectively.
Sample
  • Complex: Structure of a partial yeast 48S preinitiation complex in open conformation
    • Complex: Ribosome
      • RNA: x 1 types
      • Protein or peptide: x 34 types
    • Complex: tRNATransfer RNA
      • RNA: x 1 types
    • Complex: Initiation factors eIF1 and eIF1A
      • Protein or peptide: x 8 types
    • Complex: Initiation factors eIF2 and eIF3
      • Protein or peptide: x 2 types
    • Complex: mRNAMessenger RNA
      • RNA: x 1 types
  • Ligand: x 4 types
Function / homology
Function and homology information


: / formation of translation initiation ternary complex / Recycling of eIF2:GDP / ABC-family proteins mediated transport / eukaryotic translation initiation factor 3 complex, eIF3e / methionyl-initiator methionine tRNA binding / eukaryotic translation initiation factor 3 complex, eIF3m / translation reinitiation / eukaryotic translation initiation factor 2 complex / incipient cellular bud site ...: / formation of translation initiation ternary complex / Recycling of eIF2:GDP / ABC-family proteins mediated transport / eukaryotic translation initiation factor 3 complex, eIF3e / methionyl-initiator methionine tRNA binding / eukaryotic translation initiation factor 3 complex, eIF3m / translation reinitiation / eukaryotic translation initiation factor 2 complex / incipient cellular bud site / multi-eIF complex / eukaryotic translation initiation factor 3 complex / eukaryotic 43S preinitiation complex / cytoplasmic translational initiation / protein-synthesizing GTPase / formation of cytoplasmic translation initiation complex / formation of translation preinitiation complex / eukaryotic 48S preinitiation complex / positive regulation of translational fidelity / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / Formation of a pool of free 40S subunits / ribosomal small subunit binding / L13a-mediated translational silencing of Ceruloplasmin expression / translation regulator activity / regulation of translational fidelity / translational termination / translation initiation factor binding / translational initiation / translation initiation factor activity / cytosolic ribosome / maintenance of translational fidelity / ribosomal small subunit assembly / rRNA processing / cytoplasmic stress granule / cytosolic small ribosomal subunit / ribosome binding / double-stranded RNA binding / cytoplasmic translation / small ribosomal subunit / cytosolic large ribosomal subunit / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / GTPase activity / GTP binding / protein kinase binding / RNA binding / zinc ion binding / identical protein binding / metal ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 3 subunit G, N-terminal / eIF3G, RNA recognition motif / Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 3 subunit I / Eukaryotic translation initiation factor SUI1 / Eukaryotic translation initiation factor 3 subunit B / eIF3B, RNA recognition motif / SUI1 domain superfamily / Translation initiation factor SUI1 ...Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 3 subunit G, N-terminal / eIF3G, RNA recognition motif / Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 3 subunit I / Eukaryotic translation initiation factor SUI1 / Eukaryotic translation initiation factor 3 subunit B / eIF3B, RNA recognition motif / SUI1 domain superfamily / Translation initiation factor SUI1 / Translation initiation factor SUI1 family profile. / Eukaryotic translation initiation factor 3 subunit C, N-terminal domain / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit 8 N-terminus / Translation initiation factor, beta propellor-like domain / Eukaryotic translation initiation factor 3 subunit A / SUI1 domain / Eukaryotic translation initiation factor eIF2A / Translation initiation factor IF2/IF5 / Translation initiation factor IF2/IF5 domain / Translation initiation factor IF2/IF5, N-terminal / Translation initiation factor IF2/IF5, zinc-binding / Domain found in IF2B/IF5 / domain present in translation initiation factor eIF2B and eIF5 / Translation initiation factor 1A (eIF-1A), conserved site / Eukaryotic initiation factor 1A signature. / eukaryotic translation initiation factor 1A / Translation initiation factor 1A (eIF-1A) / IF2a, S1-like domain / Translation initiation factor 2, alpha subunit / Translation initiation factor 2, alpha subunit, middle domain superfamily / Translation initiation factor 2, alpha subunit, C-terminal / Eukaryotic translation initiation factor 2 alpha subunit / Initiation factor eIF2 gamma, domain 2 / Initiation factor eIF2 gamma, GTP-binding domain / Initiation factor eIF2 gamma, C-terminal / Initiation factor eIF2 gamma, C terminal / RNA-binding domain, S1, IF1 type / Translation initiation factor 1A / IF-1 / S1 domain IF1 type profile. / motif in proteasome subunits, Int-6, Nip-1 and TRIP-15 / PCI domain / Proteasome component (PCI) domain / PCI domain profile. / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / : / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S12e / : / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S21e, conserved site / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S19e, conserved site / S27a-like superfamily / Ribosomal protein S26e / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S25 / Ribosomal protein S26e signature. / S1 domain profile. / Ribosomal protein S17e, conserved site / : / Ribosomal protein S2, eukaryotic / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / S25 ribosomal protein / Ribosomal protein S19A/S15e / Ribosomal protein S21e signature. / Ribosomal protein S30 / Ribosomal protein S3Ae, conserved site / Ribosomal protein S12e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S8e, conserved site / 40S ribosomal protein S11, N-terminal / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S6, eukaryotic / Ribosomal protein S7e / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal S17 / Ribosomal protein S19e signature. / Ribosomal protein S19e / Ribosomal protein S27, zinc-binding domain superfamily / 40S Ribosomal protein S10 / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S27
Similarity search - Domain/homology
Eukaryotic translation initiation factor 3 subunit G / KLLA0B11231p / Eukaryotic translation initiation factor 3 subunit B / Eukaryotic translation initiation factor 2 subunit beta / Small ribosomal subunit protein eS32A / Eukaryotic translation initiation factor 2 subunit alpha / Small ribosomal subunit protein uS11 / Eukaryotic translation initiation factor 2 subunit gamma / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor eIF-1 ...Eukaryotic translation initiation factor 3 subunit G / KLLA0B11231p / Eukaryotic translation initiation factor 3 subunit B / Eukaryotic translation initiation factor 2 subunit beta / Small ribosomal subunit protein eS32A / Eukaryotic translation initiation factor 2 subunit alpha / Small ribosomal subunit protein uS11 / Eukaryotic translation initiation factor 2 subunit gamma / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor eIF-1 / Small ribosomal subunit protein eS28 / Eukaryotic translation initiation factor 3 subunit A / Eukaryotic translation initiation factor 1A / Eukaryotic translation initiation factor 3 subunit I / Ubiquitin-ribosomal protein eS31 fusion protein / Eukaryotic translation initiation factor 3 subunit G / KLLA0F25542p / KLLA0F18040p / KLLA0F09812p / KLLA0F07843p / 40S ribosomal protein S12 / Small ribosomal subunit protein eS6 / KLLA0E23673p / 40S ribosomal protein S8 / Small ribosomal subunit protein uS2 / KLLA0E12277p / 40S ribosomal protein S27 / Small ribosomal subunit protein uS14 / KLLA0D10659p / 40S ribosomal protein S3 / 40S ribosomal protein S26 / 40S ribosomal protein S7 / 40S ribosomal protein S24 / 40S ribosomal protein S30 / KLLA0B08173p / Small ribosomal subunit protein uS8 / 40S ribosomal protein S25 / Small ribosomal subunit protein eS1 / 40S ribosomal protein S4 / KLLA0B01562p / KLLA0B01474p / KLLA0A10483p / KLLA0A07194p / Small ribosomal subunit protein eS21 / RPS23 / Small ribosomal subunit protein uS9
Similarity search - Component
Biological speciesKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast) / Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) / Kluyveromyces lactis (yeast) / Saccharomyces cerevisiae S288C (yeast) / Kluyveromyces lactis NRRL Y-1140 (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 5.15 Å
AuthorsLlacer JL / Hussain T / Gordiyenko Y / Ramakrishnan V
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Wellcome TrustWT096570 United Kingdom
CitationJournal: Mol Cell / Year: 2015
Title: Conformational Differences between Open and Closed States of the Eukaryotic Translation Initiation Complex.
Authors: Jose L Llácer / Tanweer Hussain / Laura Marler / Colin Echeverría Aitken / Anil Thakur / Jon R Lorsch / Alan G Hinnebusch / V Ramakrishnan /
Abstract: Translation initiation in eukaryotes begins with the formation of a pre-initiation complex (PIC) containing the 40S ribosomal subunit, eIF1, eIF1A, eIF3, ternary complex (eIF2-GTP-Met-tRNAi), and ...Translation initiation in eukaryotes begins with the formation of a pre-initiation complex (PIC) containing the 40S ribosomal subunit, eIF1, eIF1A, eIF3, ternary complex (eIF2-GTP-Met-tRNAi), and eIF5. The PIC, in an open conformation, attaches to the 5' end of the mRNA and scans to locate the start codon, whereupon it closes to arrest scanning. We present single particle cryo-electron microscopy (cryo-EM) reconstructions of 48S PICs from yeast in these open and closed states, at 6.0 Å and 4.9 Å, respectively. These reconstructions show eIF2β as well as a configuration of eIF3 that appears to encircle the 40S, occupying part of the subunit interface. Comparison of the complexes reveals a large conformational change in the 40S head from an open mRNA latch conformation to a closed one that constricts the mRNA entry channel and narrows the P site to enclose tRNAi, thus elucidating key events in start codon recognition.
History
Header (metadata) releaseAug 24, 2016-
DepositionJun 14, 2018-
Map releaseJul 31, 2019-
UpdateFeb 22, 2023-
Current statusFeb 22, 2023Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.07
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.07
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6gsm
  • Surface level: 0.07
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_0057.png

Downloads & links

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Map

FileDownload / File: emd_0057.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationFor optimal visualization of eIF2, and of eIF3 at the 40S subunit interface, gauss-filter the map by 1.34 and display it at 0.04 or 0.03 contour level, respectively.
Voxel sizeX=Y=Z: 1.34 Å
Density
Contour LevelBy AUTHOR: 0.07 / Movie #1: 0.07
Minimum - Maximum-0.11824102 - 0.24851649
Average (Standard dev.)0.00020422891 (±0.017586468)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 402.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.341.341.34
M x/y/z300300300
origin x/y/z0.0000.0000.000
length x/y/z402.000402.000402.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS300300300
D min/max/mean-0.1180.2490.000

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Supplemental data

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Half map: #2

Fileemd_0057_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_0057_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Structure of a partial yeast 48S preinitiation complex in open co...

EntireName: Structure of a partial yeast 48S preinitiation complex in open conformation
Components
  • Complex: Structure of a partial yeast 48S preinitiation complex in open conformation
    • Complex: Ribosome
      • RNA: 18S ribosomal RNA
      • Protein or peptide: 40S ribosomal protein S0
      • Protein or peptide: 40S ribosomal protein S1
      • Protein or peptide: KLLA0F09812p
      • Protein or peptide: KLLA0D08305p
      • Protein or peptide: 40S ribosomal protein S4
      • Protein or peptide: KLLA0D10659p
      • Protein or peptide: 40S ribosomal protein S6
      • Protein or peptide: 40S ribosomal protein S7
      • Protein or peptide: 40S ribosomal protein S8
      • Protein or peptide: KLLA0E23673p
      • Protein or peptide: KLLA0B08173p
      • Protein or peptide: KLLA0A10483p
      • Protein or peptide: 40S ribosomal protein S12
      • Protein or peptide: KLLA0F18040p
      • Protein or peptide: 40S ribosomal protein S14
      • Protein or peptide: KLLA0F07843p
      • Protein or peptide: 40S ribosomal protein S16
      • Protein or peptide: KLLA0B01474p
      • Protein or peptide: KLLA0B01562p
      • Protein or peptide: KLLA0A07194p
      • Protein or peptide: KLLA0F25542p
      • Protein or peptide: 40S ribosomal protein S21
      • Protein or peptide: 40S ribosomal protein S22
      • Protein or peptide: KLLA0B11231p
      • Protein or peptide: 40S ribosomal protein S24
      • Protein or peptide: KLLA0B06182p
      • Protein or peptide: 40S ribosomal protein S26
      • Protein or peptide: 40S ribosomal protein S27
      • Protein or peptide: 40S ribosomal protein S28
      • Protein or peptide: 40S ribosomal protein S29
      • Protein or peptide: 40S ribosomal protein S30
      • Protein or peptide: Ubiquitin-40S ribosomal protein S27a
      • Protein or peptide: KLLA0E12277p
      • Protein or peptide: 60S ribosomal protein L41-ARibosome
    • Complex: tRNATransfer RNA
      • RNA: Met-tRNAi
    • Complex: Initiation factors eIF1 and eIF1A
      • Protein or peptide: Eukaryotic translation initiation factor 2 subunit alpha
      • Protein or peptide: Eukaryotic translation initiation factor 2 subunit gamma
      • Protein or peptide: Eukaryotic translation initiation factor 2 subunit beta
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit A,Eukaryotic translation initiation factor 3 subunit A,eIF3aEukaryotic initiation factor 3
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit BEukaryotic initiation factor 3
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit CEukaryotic initiation factor 3
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit IEukaryotic initiation factor 3
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit GEukaryotic initiation factor 3
    • Complex: Initiation factors eIF2 and eIF3
      • Protein or peptide: Eukaryotic translation initiation factor 1A
      • Protein or peptide: Eukaryotic translation initiation factor eIF-1
    • Complex: mRNAMessenger RNA
      • RNA: mRNA (5'-R(P*AP*AP*U)-3')
  • Ligand: [(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-2-(phosphonooxymethyl)oxolan-3-yl] (2~{S})-2-azanyl-4-methylsulfanyl-butanoate
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

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Supramolecule #1: Structure of a partial yeast 48S preinitiation complex in open co...

SupramoleculeName: Structure of a partial yeast 48S preinitiation complex in open conformation
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#47
Molecular weightTheoretical: 1.8 MDa

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Supramolecule #2: Ribosome

SupramoleculeName: Ribosome / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #2-#3, #5-#37
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)

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Supramolecule #3: tRNA

SupramoleculeName: tRNA / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #1
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)

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Supramolecule #5: Initiation factors eIF1 and eIF1A

SupramoleculeName: Initiation factors eIF1 and eIF1A / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #39-#41, #43-#47
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)

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Supramolecule #4: Initiation factors eIF2 and eIF3

SupramoleculeName: Initiation factors eIF2 and eIF3 / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #38, #42
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)

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Supramolecule #6: mRNA

SupramoleculeName: mRNA / type: complex / ID: 6 / Parent: 1 / Macromolecule list: #4
Source (natural)Organism: Kluyveromyces lactis (yeast)

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Macromolecule #1: Met-tRNAi

MacromoleculeName: Met-tRNAi / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 24.799072 KDa
SequenceString:
AGCGCCGU(1MG)(2MG) CGCAG(H2U)GGAA GCGC(M2G)CAGGG CUCAU(T6A)ACCC UGAU(7MG)(H2U)(5MC) (5MC)U CGGAUCG(1MA)AA CCG(RIA)GCGGCG CUACCA(UNK)

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Macromolecule #2: 18S ribosomal RNA

MacromoleculeName: 18S ribosomal RNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 579.239625 KDa
SequenceString: UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGAUAUCU GUGGUAAUUC U AGAGCUAA ...String:
UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGAUAUCU GUGGUAAUUC U AGAGCUAA UACAUGCUUA AAAUCUCGAC CCUUUGGAAG AGAUGUAUUU AUUAGAUAAA AAAUCAAUGU CUUCGGACUC CU UGAUGAU UCAUAAUAAC UUUUCGAAUC GCAUGGCCUU GUGCUGGCGA UGGUUCAUUC AAAUUUCUGC CCUAUCAACU UUC GAUGGU AGGAUAGUGG CCUACCAUGG UUUCAACGGG UAACGGGGAA UAAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAA CGGCU ACCACAUCCA AGGAAGGCAG CAGGCGCGCA AAUUACCCAA UCCUAAUUCA GGGAGGUAGU GACAAUAAAU AACGA UACA GGGCCCAUUC GGGUCUUGUA AUUGGAAUGA GUACAAUGUA AAUACCUUAA CGAGGAACAA CUGGAGGGCA AGUCUG GUG CCAGCAGCCG CGGUAAUUCC AGCUCCAGUA GCGUAUAUUA AAGUUGUUGC AGUUAAAAAG CUCGUAGUUG AACUUUG GG UCUGGUUGUC CGGUCCGACU UUAUGUCGCG CACUGGUUUU CAACCGGAUC UUUCCUUCUG GCUAACCUGU ACUCCUUG U GGGUGCAGGC GAACCAGGAC UUUUACUUUG AAAAAAUUAG AGUGUUCAAA GCAGGCGAAA GCUCGAAUAU AUUAGCAUG GAAUAAUGGA AUAGGACGUU UGGUUCUAUU UUGUUGGUUU CUAGGACCAU CGUAAUGAUU AAUAGGGACG GUCGGGGGCA UCAGUAUUC AAUUGUCAGA GGUGAAAUUC UUGGAUUUAU UGAAGACUAA CUACUGCGAA AGCAUUUGCC AAGGACGUUU U CAUUAAUC AAGAACGAAA GUUAGGGGAU CGAAGAUGAU CAGAUACCGU CGUAGUCUUA ACCAUAAACU AUGCCGACUA GG GAUCGGG UGGUGUUUUU CUUAUGACCC ACUCGGCACC UUACGAGAAA UCAAAGUCUU UGGGUUCUGG GGGGAGUAUG GUC GCAAGG CUGAAACUUA AAGGAAUUGA CGGAAGGGCA CCACCAGGAG UGGAGCCUGC GGCUUAAUUU GACUCAACAC GGGG AAACU CACCAGGUCC AGACACAAUA AGGAUUGACA GAUUGAGAGC UCUUUCUUGA UUUUGUGGGU GGUGGUGCAU GGCCG UUCU UAGUUGGUGG AGUGAUUUGU CUGCUUAAUU GCGAUAACGA ACGAGACCUU AACCUACUAA AUAGGGUUGC UGGCAC UUG CCGGUUGACU CUUCUUAGAG GGACUAUCGG UUUCAAGCCG AUGGAAGUUU GAGGCAAUAA CAGGUCUGUG AUGCCCU UA GACGUUCUGG GCCGCACGCG CGCUACACUG ACGGAGCCAG CGAGUACAAC CUUGGCCGAG AGGUCUGGGU AAUCUUGU G AAACUCCGUC GUGCUGGGGA UAGAGCAUUG UAAUUAUUGC UCUUCAACGA GGAAUUCCUA GUAAGCGCAA GUCAUCAGC UUGCGUUGAU UACGUCCCUG CCCUUUGUAC ACACCGCCCG UCGCUAGUAC CGAUUGAAUG GCUUAGUGAG GCCUCAGGAU UUGCUUAGA GAAGGGGGCA ACUCCAUCUC AGAGCGAAGA AUCUGGUCAA ACUUGGUCAU UUAGAGGAAC UAAAAGUCGU A ACAAGGUU UCCGUAGGUG AACCUGCGGA AGGAUCAUUA

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Macromolecule #4: mRNA (5'-R(P*AP*AP*U)-3')

MacromoleculeName: mRNA (5'-R(P*AP*AP*U)-3') / type: rna / ID: 4 / Number of copies: 1
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 919.62 Da
SequenceString:
AAU

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Macromolecule #3: 40S ribosomal protein S0

MacromoleculeName: 40S ribosomal protein S0 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 23.140398 KDa
SequenceString: SLPSTFDLTS EDAQLLLAAR VHLGAKNVQV HQEPYVYKAR PDGVNVINVG KTWEKIVLAA RIIAAIPNPE DVVAISSRTY GQRAVLKYA AHTGATPIAG RFTPGSFTNY ITRSFKEPRL VIVTDPRSDA QAIKESSYVN IPVIALTDLD SPSEYVDVAI P CNNRGKHS ...String:
SLPSTFDLTS EDAQLLLAAR VHLGAKNVQV HQEPYVYKAR PDGVNVINVG KTWEKIVLAA RIIAAIPNPE DVVAISSRTY GQRAVLKYA AHTGATPIAG RFTPGSFTNY ITRSFKEPRL VIVTDPRSDA QAIKESSYVN IPVIALTDLD SPSEYVDVAI P CNNRGKHS IGLIWYLLAR EVLRLRGALP DRTQPWAIMP DLYFYRNPEE

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Macromolecule #5: 40S ribosomal protein S1

MacromoleculeName: 40S ribosomal protein S1 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 26.404725 KDa
SequenceString: VGKNKRLSKG KKGLKKRVVD PFTRKEWYDI KAPSTFENRN VGKTLVNKSV GLKNASDSLK GRVVEVCLAD LQGSEDHSFR KVKLRVDEV QGKNLLTNFH GMDFTTDKLR SMVRKWQTLI EANVTVKTSD DYVLRIFAIA FTRKQANQVK RTSYAQSSHI R QIRKVISE ...String:
VGKNKRLSKG KKGLKKRVVD PFTRKEWYDI KAPSTFENRN VGKTLVNKSV GLKNASDSLK GRVVEVCLAD LQGSEDHSFR KVKLRVDEV QGKNLLTNFH GMDFTTDKLR SMVRKWQTLI EANVTVKTSD DYVLRIFAIA FTRKQANQVK RTSYAQSSHI R QIRKVISE ILTREVQNST LAQLTSKLIP EVINKEIENA TKDIFPLQNV HIRKVKLLKQ PKFDLGSLLS LHG

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Macromolecule #6: KLLA0F09812p

MacromoleculeName: KLLA0F09812p / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 23.162922 KDa
SequenceString: GWVPVTKLGR LVKAGKISSI EEIFLHSLPV KEFQIIDQLL PNLKDEVMNI KPVQKQTRAG QRTRFKAVVV VGDSNGHVGL GIKTAKEVA GAIRAGIIIA KLSVIPIRRG YWGTNLGQPH SLATKTSGKC GSVSVRLIPA PRGSGIVASP AVKKLMQLAG V EDVYTSST ...String:
GWVPVTKLGR LVKAGKISSI EEIFLHSLPV KEFQIIDQLL PNLKDEVMNI KPVQKQTRAG QRTRFKAVVV VGDSNGHVGL GIKTAKEVA GAIRAGIIIA KLSVIPIRRG YWGTNLGQPH SLATKTSGKC GSVSVRLIPA PRGSGIVASP AVKKLMQLAG V EDVYTSST GSTRTLENTL KAAFVAIGNT YGFLTPNLWE VQALTPSPMD VYADYATAS

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Macromolecule #7: KLLA0D08305p

MacromoleculeName: KLLA0D08305p / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 24.830895 KDa
SequenceString: AIISKKRKLV ADGVFYAELN EFFTRELAEE GYSGVEVRVT PTKTEIIIRA TKVQDVVGEN GRRINELTLL IEKRFKYKRG TIALYAERV HDRGLSAVAQ AESMKFKLLN GLAIRRAAYG VVRYVMESGA KGCEVVISGK LRAARAKSMK FADGFLIHSG Q PVNDFIET ...String:
AIISKKRKLV ADGVFYAELN EFFTRELAEE GYSGVEVRVT PTKTEIIIRA TKVQDVVGEN GRRINELTLL IEKRFKYKRG TIALYAERV HDRGLSAVAQ AESMKFKLLN GLAIRRAAYG VVRYVMESGA KGCEVVISGK LRAARAKSMK FADGFLIHSG Q PVNDFIET ATRHVLLRQG VLGIKVKIMK DPSRNTSGPK ALPDAVTIIE PKEEEPVLEP SVKDY

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Macromolecule #8: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 29.486312 KDa
SequenceString: ARGPKKHLKR LAAPHHWMLD KLSGCYAPRP SAGPHKLRES LPLIVFLRNR LKYALNGREV KAILMQRHVK VDGKVRTDTT FPAGFMDVI TLEATNENFR LVYDVKGRFA VHRITDEEAS YKLAKVKKVQ LGKKGIPYVV THDGRTIRYP DPNIKVNDTV K VDLATGTI ...String:
ARGPKKHLKR LAAPHHWMLD KLSGCYAPRP SAGPHKLRES LPLIVFLRNR LKYALNGREV KAILMQRHVK VDGKVRTDTT FPAGFMDVI TLEATNENFR LVYDVKGRFA VHRITDEEAS YKLAKVKKVQ LGKKGIPYVV THDGRTIRYP DPNIKVNDTV K VDLATGTI TDFIKFDTGK LVYVTGGRNL GRVGTIVHRE RHEGGFDLVH IKDSLENTFV TRLNNVFVIG EPGRPWISLP KG KGIKLTI SEERDRRRAQ HGL

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Macromolecule #9: KLLA0D10659p

MacromoleculeName: KLLA0D10659p / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 22.912369 KDa
SequenceString: FVPVELATTI PVEIQQAQQE IKLFNKWSFE DVEVKDASLV DYIQISKPIY VAHTAGRYAN KRFRKAQCPI VERLTNSLMM NGRNNGKKL KAVRIVKHTL EIINVLTDQN PLQVVVDAII NSGPREDTTR VGGGGAARRQ AVDVSPLRRV NQSIALLTIG A REAAFRNI ...String:
FVPVELATTI PVEIQQAQQE IKLFNKWSFE DVEVKDASLV DYIQISKPIY VAHTAGRYAN KRFRKAQCPI VERLTNSLMM NGRNNGKKL KAVRIVKHTL EIINVLTDQN PLQVVVDAII NSGPREDTTR VGGGGAARRQ AVDVSPLRRV NQSIALLTIG A REAAFRNI KTIAETLAEE LINAAKGSST SYAIKKKDEL ERVAKSNR

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Macromolecule #10: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 25.810977 KDa
SequenceString: MKLNISYPIN GTQKCIEIDD EHRVRVFYDK RIGQEVDGES VGDEFKGYVF KIAGGNDKQG FPMKQGVLLP TRVKLLLAKG HSCYRPRRN GERKRKSVRG AIVGPDLAVL ALIITKKGEQ EIEGITNDTV PKRLGPKRAN NIRKFFGLTK EDDVRDYVIR R EVTKGDKS ...String:
MKLNISYPIN GTQKCIEIDD EHRVRVFYDK RIGQEVDGES VGDEFKGYVF KIAGGNDKQG FPMKQGVLLP TRVKLLLAKG HSCYRPRRN GERKRKSVRG AIVGPDLAVL ALIITKKGEQ EIEGITNDTV PKRLGPKRAN NIRKFFGLTK EDDVRDYVIR R EVTKGDKS YTKAPKIQRL VTPQRLQRKR QQKSLKIKNA QAQREAAAEY AQLLAKRLSE RKAEKAEV

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Macromolecule #11: 40S ribosomal protein S7

MacromoleculeName: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 21.03357 KDa
SequenceString:
PQAKILSQAP TELELQVAQA FIDLENNSPE LKADLRALQF KSIREIEVAG GKKALAVFVP VPSLAAYHKV QIKLTRELEK KFQDRHVIF LAERRILPKP SRKSRQTQKR PRSRTLTAVH DKILEDLVFP TEIVGKRVRY LVGGNKIQKI LLNSKDVQHI D NKLESFQA VYNKLTGKQI VFEIPS

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Macromolecule #12: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 22.511531 KDa
SequenceString: GISRDSRHKR AATGAKRAQF RKKRKFELGR QAANTKIGTK RIHPVRTRGG NQKFRALRIE TGNFSWASEG VARKTRITGV VYHPSNNEL VRTNTLTKAA IVQIDATPFR QWYESHYGQS LGKKKNTKAE EETATTSKNT ERKWAARAAE AKIEHAVDSQ F GAGRLYAA ...String:
GISRDSRHKR AATGAKRAQF RKKRKFELGR QAANTKIGTK RIHPVRTRGG NQKFRALRIE TGNFSWASEG VARKTRITGV VYHPSNNEL VRTNTLTKAA IVQIDATPFR QWYESHYGQS LGKKKNTKAE EETATTSKNT ERKWAARAAE AKIEHAVDSQ F GAGRLYAA ISSRPGQSGR CDGYILEGEE LAFYLRRLTA KK

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Macromolecule #13: KLLA0E23673p

MacromoleculeName: KLLA0E23673p / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 20.882344 KDa
SequenceString:
PRAPRTYSKT YSTPKRPYES ARLDAELKLA GEYGLKNKRE IYRISFQLSK IRRAARDLLT RDEKDPKRLF EGNALIRRLV RIGVLSEDK KKLDYVLALK VEDFLERRLQ TQVYKLGLAK SVHHARVLIS QRHIAVGKQI VNIPSFMVRL ESEKHIDFAR T SPFGGARP GRVARKRAAA AGGE

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Macromolecule #14: KLLA0B08173p

MacromoleculeName: KLLA0B08173p / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 11.423085 KDa
SequenceString:
MLIPKEDRKK IYQHLFQEGV LVAKKDFNQP KHEEIDTKNL FVIKALQSLT SKGFVKTQFS WQYYYYTLTE EGVVYLREYL NLPEHIFPA TYLAGQS

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Macromolecule #15: KLLA0A10483p

MacromoleculeName: KLLA0A10483p / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 17.712734 KDa
SequenceString:
STELTVQSER AFQKQPHIFT NPKAKANRKT KRWYKNVGLG FKTPKTAIEG SYIDKKCPFT GLVSIRGKIL TGTVVSTRMH RTIVIRRDY LHYVPKYNRY EKRHKNVPAH VSPAFRVQVG DIVTVGQCRP ISKTVRFNVL KVASATGKAN KQFAKF

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Macromolecule #16: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 12.710457 KDa
SequenceString:
IEDALKVVLR TSLVHDGLAR GLRESAKALT RGEGQLAVLV ESVTEEAISK LVQGLATENN VPLIKVADAK QLGEWAGLGK IDRDGNARK VVGASVVVVK NWGADTQERE ILLEHFSQQ

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Macromolecule #17: KLLA0F18040p

MacromoleculeName: KLLA0F18040p / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 16.858678 KDa
SequenceString:
GRMHSKGKGM SSSAIPYSRN APAWFKGSSD GVVEQIIKYA RKGLTPSQIG VLLRDAHGVT QAKVITGNKI LRILKSNGLA PEIPEDLYF LIKKAVSVRK HLERNRKDKD AKFRLILIES RIHRLARYYR TVSVLPPNWK YESATASALV N

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Macromolecule #18: 40S ribosomal protein S14

MacromoleculeName: 40S ribosomal protein S14 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 13.458439 KDa
SequenceString:
SQVFGVARIF ASFNDTFVHV TDLSGRETIA RVTGGMKVKA DRDESSPYAA MLAAQDVAAK CKEVGITAVH IKIRATGGTR SKTPGPGGQ AALRALARSG LRIGRIEDVT PVPSDSTRKK GGRRGRRL

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Macromolecule #19: KLLA0F07843p

MacromoleculeName: KLLA0F07843p / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 13.448831 KDa
SequenceString:
KTYSYKGVDL EKLLEMPTED FVKLAPARVR RKFARGLSEK PAGLMKKLRA AKLSAPENEK PAVVRTHLRN MIIVPEMIGS VVGVYNGKV FNQVEIRPEM VGHYLGEFSI TYTPVRHGRA

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Macromolecule #20: 40S ribosomal protein S16

MacromoleculeName: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 15.656257 KDa
SequenceString:
TVPSVQTFGK KKSATAVAHV KAGKGLIKVN GSPITLVQPE ILRFKVYEPL LLVGLDKFAN IDIRVKVTGG GHVSQVYAIR QAIAKGLVA YHQKFVDEQS KNELKKAFTS YDRTLLIADS RRPEPKKFGG RGARSRFQKS YR

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Macromolecule #21: KLLA0B01474p

MacromoleculeName: KLLA0B01474p / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 14.200373 KDa
SequenceString:
GRVRTKTVKR ASKALIEKYY PKLTMDFQTN KRLCDEIATI QSKRLRNKIA GYTTHLMKRI QKGPVRGISF KLQEEERERK DQYVPDVSA LDLSHSNDVL NVDTQTAELV NSLGLKLPLS VSSVSA

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Macromolecule #22: KLLA0B01562p

MacromoleculeName: KLLA0B01562p / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 16.953404 KDa
SequenceString:
SLVVQEQGSF QHILRLLNTN VDGNINVVYA LTTIRGVGRR YANLVCKKAD VDLHKRAGEL TQEELERIVQ IMQNPTHYKI PAWFLNRQK DVNDGKDYHS LANNLESKLR DDLERLKKIR SHRGIRHFWG LRVRGQHTKT TGRRRA

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Macromolecule #23: KLLA0A07194p

MacromoleculeName: KLLA0A07194p / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 15.747814 KDa
SequenceString:
PGVSVRDVPA QDFINNYASF LQRQGKLEVP GYVDIVKTSA GNELPPQDSE GWFYKRAASV ARHIYLRKQV GVGKLNKLYG GAKNRGVRP HKHVDASGSI NRKVLQSLEK LGVVEISPKG GRRISDNGLR DLDRIAAATL EDEE

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Macromolecule #24: KLLA0F25542p

MacromoleculeName: KLLA0F25542p / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 12.032146 KDa
SequenceString:
QEVVIHKIRI NLTSTKVKQL ENVSANIIKN AETFKLVKKG PVRLPTKVLK ISTRKTPNGE GSKTWDTYEM RIHKRYIDLE APAHIVKRI TQITIEPGVD VEVIIAA

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Macromolecule #25: 40S ribosomal protein S21

MacromoleculeName: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 9.797949 KDa
SequenceString:
MENDKGQLVE LYVPRKCSAT NRIIKAKDHS SVQINIAQVD EEGRAIPGEY VTYALSGYIR ARGEADDSLN RLAQQDGLLK NVWSYSR

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Macromolecule #26: 40S ribosomal protein S22

MacromoleculeName: 40S ribosomal protein S22 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 14.513846 KDa
SequenceString:
TRTSVLADAL NAINNAEKTG KRQVLIRPSS KVIIKFLQVM QKHGYIGEFE YIDDHRSGKI VVQLNGRLNK CGVISPRFNV KIADVEKWT ANLLPARQFG YVILTTSAGI MDHEEAHRKH VSGKILGFVY

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Macromolecule #27: KLLA0B11231p

MacromoleculeName: KLLA0B11231p / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 15.916701 KDa
SequenceString:
GKGKPRGLNS ARKLRVHRRN NRWAETTYKK RLLGTAFKSS PFGGSSHAKG IVLEKIGIES KQPNSAIRKC VRVQLIKNGK KVTAFVPND GCLNFVDEND EVLLAGFGRK GKAKGDIPGV RFKVVKVSGV SLLALWKEKK EKPRS

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Macromolecule #28: 40S ribosomal protein S24

MacromoleculeName: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 15.063354 KDa
SequenceString:
SDAITIRTRK VISNPLLARK QFVVDVLHPN RANVSKDELR EKLAEAYKAE KDAVSVFGFR TQYGGGKSTG FGLVYNSVAD AKKFEPAYR LVRYGLAEKV EKASRQQRKQ RKNRGKKIFG TGKSIAKKAA RRNAD

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Macromolecule #29: KLLA0B06182p

MacromoleculeName: KLLA0B06182p / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 7.939246 KDa
SequenceString:
AKHAVVLDQD KFDRIMKEAP TYRYVSVSVL VDRFKLGGSL ARVALRHLEN EGIIKPVSKH SKQAIYTRAT

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Macromolecule #30: 40S ribosomal protein S26

MacromoleculeName: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 11.166179 KDa
SequenceString:
PKKRASNGRN KKGRGHVKPV RCVNCSRSVP KDKAIKRMAI RNIVEAAAIR DLSEASVYAE YALPKTYNKL HYCISCAIHA RIVRVRSRT DRRIRAPPQ

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Macromolecule #31: 40S ribosomal protein S27

MacromoleculeName: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 8.753168 KDa
SequenceString:
VLVQDLLHPT AASEARKHKL KTLVQSPRSH FLDVKCPGCL NITTVFSHAQ TAVTCESCST VLCTPTGGKA KLSEGTSFRR K

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Macromolecule #32: 40S ribosomal protein S28

MacromoleculeName: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 6.972152 KDa
SequenceString:
PVTLAKVIKV LGRTGSRGGV TQVRVEFLED TTRTIVRNVK GPVREGDILV LMESEREARR LR

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Macromolecule #33: 40S ribosomal protein S29

MacromoleculeName: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 6.322149 KDa
SequenceString:
ENVWYSHPRK FGKGSRQCRI SGSHSGLIRK YGLNIDRQSF REKANDIGFY KYR

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Macromolecule #34: 40S ribosomal protein S30

MacromoleculeName: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 6.608788 KDa
SequenceString:
VHGSLARAGK VKSQTPKVEK QEKPKQPKGR AYKRLLYTRR FVNVTLTNGK RKMNPSPS

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Macromolecule #35: Ubiquitin-40S ribosomal protein S27a

MacromoleculeName: Ubiquitin-40S ribosomal protein S27a / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 7.958424 KDa
SequenceString:
KKVYTTPKKI RHKHKKVKLA VLNYYKVDDE GKVAKLRKEC PNCGPGIFLA NHGDRFYCGK CHSTFATQK

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Macromolecule #36: KLLA0E12277p

MacromoleculeName: KLLA0E12277p / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37
Molecular weightTheoretical: 35.612676 KDa
SequenceString: SSNIMLVLRG TLEGHNGWVT SLSTSAAQPN LLVSGSRDKT LISWRLTENE QQFGVPVRSY KGHSHIVQDV VVSADGNYAV SASWDKTLR LWNLATGNSE ARFVGHTGDV LSVAIDANSS KIISASRDKT IRVWNTVGDC AYVLLGHTDW VTKVRVAPKN L EDGEVDDG ...String:
SSNIMLVLRG TLEGHNGWVT SLSTSAAQPN LLVSGSRDKT LISWRLTENE QQFGVPVRSY KGHSHIVQDV VVSADGNYAV SASWDKTLR LWNLATGNSE ARFVGHTGDV LSVAIDANSS KIISASRDKT IRVWNTVGDC AYVLLGHTDW VTKVRVAPKN L EDGEVDDG RITFVSAGMD KIVRSWSLNE DSYRIEADFI GHNNYINVVQ PSPDGSLAAS AGKDGQIYVW NLKHKSAFMN FD AKDEVFA LAFSPSRFWL TAATASGIKI YDLENEVLID ELKPEFAGYT KAQDPHAVSL AWSADGQTLF AGYTDNVIRV WQV MTAN

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Macromolecule #37: 60S ribosomal protein L41-A

MacromoleculeName: 60S ribosomal protein L41-A / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 3.354243 KDa
SequenceString:
MRAKWRKKRT RRLKRKRRKV RARSK

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Macromolecule #38: Eukaryotic translation initiation factor 1A

MacromoleculeName: Eukaryotic translation initiation factor 1A / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 10.953535 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString:
GPKRELIYKE EGQEYAQITK MLGNGRVEAS CFDGNKRMAH IRGKLRKKVW MGQGDIILVS LRDFQDDQCD VVHKYNLDEA RTLKNQGEL PENAKI

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Macromolecule #39: Eukaryotic translation initiation factor 2 subunit alpha

MacromoleculeName: Eukaryotic translation initiation factor 2 subunit alpha
type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 30.221979 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: TSHCRFYENK YPEIDDIVMV NVQQIAEMGA YVKLLEYDNI EGMILLSELS RRRIRSIQKL IRVGKNDVAV VLRVDKEKGY IDLSKRRVS SEDIIKCEEK YQKSKTVHSI LRYCAEKFQI PLEELYKTIA WPLSRKFGHA YEAFKLSIID ETVWEGIEPP S KDVLDELK ...String:
TSHCRFYENK YPEIDDIVMV NVQQIAEMGA YVKLLEYDNI EGMILLSELS RRRIRSIQKL IRVGKNDVAV VLRVDKEKGY IDLSKRRVS SEDIIKCEEK YQKSKTVHSI LRYCAEKFQI PLEELYKTIA WPLSRKFGHA YEAFKLSIID ETVWEGIEPP S KDVLDELK NYISKRLTPQ AVKIRADVEV SCFSYEGIDA IKDALKSAED MSTEQMQVKV KLVAAPLYVL TTQALDKQKG IE QLESAIE KITEVITKYG GVCNIT

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Macromolecule #40: Eukaryotic translation initiation factor 2 subunit gamma

MacromoleculeName: Eukaryotic translation initiation factor 2 subunit gamma
type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 47.230148 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: LSAEIINRQA TINIGTIGHV AHGKSTVVRA ISGVQTVRFK DELERNITIK LGYANAKIYK CQEPTCPEPD CYRSFKSDKE ISPKCQRPG CPGRYKLVRH VSFVDCPGHD ILMSTMLSGA AVMDAALLLI AGNESCPQPQ TSEHLAAIEI MKLKHVIILQ N KVDLMREE ...String:
LSAEIINRQA TINIGTIGHV AHGKSTVVRA ISGVQTVRFK DELERNITIK LGYANAKIYK CQEPTCPEPD CYRSFKSDKE ISPKCQRPG CPGRYKLVRH VSFVDCPGHD ILMSTMLSGA AVMDAALLLI AGNESCPQPQ TSEHLAAIEI MKLKHVIILQ N KVDLMREE SALEHQKSIL KFIRGTIADG APIVPISAQL KYNIDAVNEF IVKTIPVPPR DFMISPRLIV IRSFDVNKPG AE IEDLKGG VAGGSILNGV FKLGDEIEIR PGIVTKDDKG KIQCKPIFSN IVSLFAEQND LKFAVPGGLI GVGTKVDPTL CRA DRLVGQ VVGAKGHLPN IYTDIEINYF LLRRLLGVKT DGQKQAKVRK LEPNEVLMVN IGSTATGARV VAVKADMARL QLTS PACTE INEKIALSRR IEKHWRLIGW ATIKK

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Macromolecule #41: Eukaryotic translation initiation factor 2 subunit beta

MacromoleculeName: Eukaryotic translation initiation factor 2 subunit beta
type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 16.45809 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
VGLPYSELLS RFFNILRTNN PELAGDRSGP KFRIPPPVCL RDGKKTIFSN IQDIAEKLHR SPEHLIQYLF AELGTSGSVD GQKRLVIKG KFQSKQMENV LRRYILEYVT CKTCKSINTE LKREQSNRLF FMVCKSCGST RSVSS

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Macromolecule #42: Eukaryotic translation initiation factor eIF-1

MacromoleculeName: Eukaryotic translation initiation factor eIF-1 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 10.919531 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString:
ADTGDDETAT SNYIHIRIQQ RNGRKTLTTV QGVPEEYDLK RILKVLKKDF ACNGNIVKDP EMGEIIQLQG DQRAKVCEFM ISQLGLQKK NIKIHGF

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Macromolecule #43: Eukaryotic translation initiation factor 3 subunit A,Eukaryotic t...

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit A,Eukaryotic translation initiation factor 3 subunit A,eIF3a
type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 62.822781 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: PFRPENAIKR ADELISVGEK QAALQSLHDF ITARRIRWAT PSTVEPVVFK FLEIGVELKK GKLLKDGLHQ YKKLIQGSTE GLVSVGAVA RKFIDLVESK IASEQTRADE LQKQEIDDDL EGGVTPENLL ISVYESDQSV AGFNDEAITS WLRFTWESYR A VLDLLRNN ...String:
PFRPENAIKR ADELISVGEK QAALQSLHDF ITARRIRWAT PSTVEPVVFK FLEIGVELKK GKLLKDGLHQ YKKLIQGSTE GLVSVGAVA RKFIDLVESK IASEQTRADE LQKQEIDDDL EGGVTPENLL ISVYESDQSV AGFNDEAITS WLRFTWESYR A VLDLLRNN ALLEITYSGV VKKTMHFCLK YQRKNEFKRL AEMLRQHLDA ANYQQSKSGN NLVDLSDADT LQRYLDQRFQ QV DVSVKLE LWHEAYRSIE DVFHLMKISK RAPKPSTLAN YYENLVKVFF VSGDPLLHTT AWKKFYKLYS TNPRATEEEF KTY SSTIFL SAISTQLDEI PSIGYDPHLR MYRLLNLDAK PTRKEMLQSI IEDESIYGKV DEELKELYDI IEVNFDVDTV KQQL ENLLV KLSSKTYFSQ YIAPLRDVIM RRVFVAASQK FTTVSQSELY KLATLPAPLD LSAWDIEKSL LQAAVEDYVS ITIDH ESAK VTFA(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

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Macromolecule #44: Eukaryotic translation initiation factor 3 subunit B

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit B / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 75.014 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: QYIVVNGAPV IPSAKVPVLK KALTSLFSKA GKVVNMEFPI DEATGKTKGF LFVECGSMND AKKIIKSFHG KRLDLKHRLF LYTMKDVER YNSDDFDTEF REPDMPTFVP SSSLKSWLMD DKVRDQFVLQ DDVKTSVFWN SMFNEEDSLV ESRENWSTNY V RFSPKGTY ...String:
QYIVVNGAPV IPSAKVPVLK KALTSLFSKA GKVVNMEFPI DEATGKTKGF LFVECGSMND AKKIIKSFHG KRLDLKHRLF LYTMKDVER YNSDDFDTEF REPDMPTFVP SSSLKSWLMD DKVRDQFVLQ DDVKTSVFWN SMFNEEDSLV ESRENWSTNY V RFSPKGTY LFSYHQQGVT AWGGPNFDRL RRFYHPDVRN SSVSPNEKYL VTFSTEPIIV EEDNEFSPFT KKNEGHQLCI WD IASGLLM ATFPVIKSPY LKWPLVRWSY NDKYCARMVG DSLIVHDATK NFMPLEAKAL KPSGIRDFSF APEGVKLQPF RNG DEPSVL LAYWTPETNN SACTATIAEV PRGRVLKTVN LVQVSNVTLH WQNQAEFLCF NVERHTKSGK TQFSNLQICR LTER DIPVE KVELKDSVFE FGWEPHGNRF VTISVHEVAD MNYAIPANTI RFYAPETKEK TDVIKRWSLV KEIPKTFANT VSWSP AGRF VVVGALVGPN MRRSDLQFYD MDYPGEKNIN DNNDVSASLK DVAHPTYSAA TNITWDPSGR YVTAWSSSLK HKVEHG YKI FNIAGNLVKE DIIAGFKNFA WRPRPLSNAE RKKVRKNLRE WSEEQDAMEA DTAMRHQREL LKQWTEYREK IGQEMEK SM NFKIFDVQP

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Macromolecule #45: Eukaryotic translation initiation factor 3 subunit C

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit C / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 76.96318 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: YDSSDEESDE EDGKKVVKSA KEKLLDEMQD VYNKISQAEN SDWLTISNEF DLISRLLVRA QQQNWGTPNI FIKVVAQVED AVNNTQQAD LKAVARAYNT TKQRVKKVSR ENEDSMAKFR NDQEDFFTRL QTIIDSRGKK TVNQQSLIST LEELLTVAEK P YEFIMAYL ...String:
YDSSDEESDE EDGKKVVKSA KEKLLDEMQD VYNKISQAEN SDWLTISNEF DLISRLLVRA QQQNWGTPNI FIKVVAQVED AVNNTQQAD LKAVARAYNT TKQRVKKVSR ENEDSMAKFR NDQEDFFTRL QTIIDSRGKK TVNQQSLIST LEELLTVAEK P YEFIMAYL TLIPSRFDAS ANLSYQPIDQ WKSSFNDISK LLSILDQTID TYQVNEFADP IDFIEDEPKE DSDGVKRILG SI FSFVERL DDEFMKSLLN IDPHSSDYLI RLRDEQSIYN LILRTQLYFE ATLKDEHDLE RALTRPFVKR LDHIYYKSEN LIK IMETAA WNIIPAQFKS KFTSKDQLDS ADYVDNLIDG LSTILSKQNN IAVQKRAILY NIYYTALNKD FQTAKDMLLT SQVQ TNINQ FDSSLQILFN RVVVQLGLSA FKLCLIEECH QILNDLLSSS HLREILGQQS LHRISLNSSN NASADERARQ CLPYH QHIN LDLIDVVFLT CSLLIEIPRM TAFYSGIKVK RIPYSPKSIR RSLEHYDKLS FQGPPETLRD YVLFAAKSMQ KGNWRD SVK YLREIKSWAL LPNMETVLNS LTERVQVESL KTYFFSFKRF YSSFSVAKLA ELFDLPENKV VEVLQSVIAE LEIPAKL ND EKTIFVVEKG DEITKLEEAM VKL

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Macromolecule #46: Eukaryotic translation initiation factor 3 subunit I

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit I / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 38.229754 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MKAIKLTGHE RPLTQVKYNK EGDLLFSCSK DSSASVWYSL NGERLGTLDG HTGTIWSIDV DCFTKYCVTG SADYSIKLWD VSNGQCVAT WKSPVPVKRV EFSPCGNYFL AILDNVMKNP GSINIYEIER DSATHELTKV SEEPIHKIIT HEGLDAATVA G WSTKGKYI ...String:
MKAIKLTGHE RPLTQVKYNK EGDLLFSCSK DSSASVWYSL NGERLGTLDG HTGTIWSIDV DCFTKYCVTG SADYSIKLWD VSNGQCVAT WKSPVPVKRV EFSPCGNYFL AILDNVMKNP GSINIYEIER DSATHELTKV SEEPIHKIIT HEGLDAATVA G WSTKGKYI IAGHKDGKIS KYDVSNNYEY VDSIDLHEKS ISDMQFSPDL TYFITSSRDT NSFLVDVSTL QVLKKYETDC PL NTAVITP LKEFIILGGG QEAKDVTTTS ANEGKFEARF YHKIFEEEIG RVQGHFGPLN TVAISPQGTS YASGGEDGFI RLH HFEKSY FDFKYDVEKA AEAKEH

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Macromolecule #47: Eukaryotic translation initiation factor 3 subunit G

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit G / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 5.576158 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
SVAERKNWHK YGSEKGSPAG PSAVTARLGE EVELRLSRNW KQAEEERIQ

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Macromolecule #48: [(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-2-(ph...

MacromoleculeName: [(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-2-(phosphonooxymethyl)oxolan-3-yl] (2~{S})-2-azanyl-4-methylsulfanyl-butanoate
type: ligand / ID: 48 / Number of copies: 1 / Formula: 7NO
Molecular weightTheoretical: 478.417 Da
Chemical component information

ChemComp-7NO:
[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-2-(phosphonooxymethyl)oxolan-3-yl] (2~{S})-2-azanyl-4-methylsulfanyl-butanoate

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Macromolecule #49: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 49 / Number of copies: 82 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #50: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 50 / Number of copies: 4 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #51: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

MacromoleculeName: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / type: ligand / ID: 51 / Number of copies: 1 / Formula: GCP
Molecular weightTheoretical: 521.208 Da
Chemical component information

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.15 mg/mL
BufferpH: 6.5
Component:
ConcentrationName
20.0 mMMES
5.0 mMMagnessium acetate
80.0 mMPotassium acetate
10.0 mMAmmonium acetate
2.0 mMDithiothreitol (DTT)
0.001 mMZinc acetate
0.6 mMATPAdenosine triphosphate
0.25 mMGDPCP
GridModel: Quantifoil R2/2 / Support film - Material: CARBON / Support film - topology: CONTINUOUS
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK I

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 59000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
TemperatureMin: 90.0 K / Max: 100.0 K
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Number grids imaged: 2 / Number real images: 2610 / Average exposure time: 1.1 sec. / Average electron dose: 40.0 e/Å2
Details: Images were collected in movie-mode at 40 frames per second
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 360729
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

4v88

Initial angle assignmentType: NOT APPLICABLE / Software - Name: RELION (ver. 1.4)
Final 3D classificationSoftware - Name: RELION (ver. 1.4)
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 1.4)
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 5.15 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.4) / Number images used: 5750
DetailsFEI Falcon III

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Atomic model buiding 1

RefinementSpace: RECIPROCAL / Protocol: OTHER / Target criteria: FSC
Output model

PDB-6gsm:
Structure of a partial yeast 48S preinitiation complex in open conformation.

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