ジャーナル: J Mol Biol / 年: 2018 タイトル: Structural Insights into the Role of Diphthamide on Elongation Factor 2 in mRNA Reading-Frame Maintenance. 著者: Simone Pellegrino / Natalia Demeshkina / Eder Mancera-Martinez / Sergey Melnikov / Angelita Simonetti / Alexander Myasnikov / Marat Yusupov / Gulnara Yusupova / Yaser Hashem / 要旨: One of the most critical steps of protein biosynthesis is the coupled movement of mRNA, which encodes genetic information, with tRNAs on the ribosome. In eukaryotes, this process is catalyzed by a ...One of the most critical steps of protein biosynthesis is the coupled movement of mRNA, which encodes genetic information, with tRNAs on the ribosome. In eukaryotes, this process is catalyzed by a conserved G-protein, the elongation factor 2 (eEF2), which carries a unique post-translational modification, called diphthamide, found in all eukaryotic species. Here we present near-atomic resolution cryo-electron microscopy structures of yeast 80S ribosome complexes containing mRNA, tRNA and eEF2 trapped in different GTP-hydrolysis states which provide further structural insights into the role of diphthamide in the mechanism of translation fidelity in eukaryotes.
ダウンロード / ファイル: emd_0055.map.gz / 形式: CCP4 / 大きさ: 178 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
ボクセルのサイズ
X=Y=Z: 1.1 Å
密度
表面レベル
登録者による: 0.035 / ムービー #1: 0.035
最小 - 最大
-0.16047029 - 0.308272
平均 (標準偏差)
0.0010391705 (±0.010803084)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
360
360
360
Spacing
360
360
360
セル
A=B=C: 396.0 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
1.1
1.1
1.1
M x/y/z
360
360
360
origin x/y/z
0.000
0.000
0.000
length x/y/z
396.000
396.000
396.000
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
360
360
360
D min/max/mean
-0.160
0.308
0.001
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添付データ
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試料の構成要素
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全体 : 80S ribosome in complex with mRNA, tRNA and eEF2 in presence of G...
全体
名称: 80S ribosome in complex with mRNA, tRNA and eEF2 in presence of GDP, AlF4 and anti-fungal drug (sordarin)
要素
複合体: 80S ribosome in complex with mRNA, tRNA and eEF2 in presence of GDP, AlF4 and anti-fungal drug (sordarin)
複合体: 80S ribosome
複合体: mRNA
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超分子 #1: 80S ribosome in complex with mRNA, tRNA and eEF2 in presence of G...
超分子
名称: 80S ribosome in complex with mRNA, tRNA and eEF2 in presence of GDP, AlF4 and anti-fungal drug (sordarin) タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1