+検索条件
-Structure paper
タイトル | Addressing compressive deformation of proteins embedded in crystalline ice. |
---|---|
ジャーナル・号・ページ | Structure, Vol. 31, Issue 2, Page 213-220.e3, Year 2023 |
掲載日 | 2023年2月2日 |
著者 | Huigang Shi / Chunling Wu / Xinzheng Zhang / |
PubMed 要旨 | For cryoelectron microscopy (cryo-EM), high cooling rates have been required for preparation of protein samples to vitrify the surrounding water and avoid formation of damaging crystalline ice. ...For cryoelectron microscopy (cryo-EM), high cooling rates have been required for preparation of protein samples to vitrify the surrounding water and avoid formation of damaging crystalline ice. Whether and how crystalline ice affects single-particle cryo-EM is still unclear. Here, single-particle cryo-EM was used to analyze three-dimensional structures of various proteins and viruses embedded in crystalline ice formed at various cooling rates. Low cooling rates led to shrinkage deformation and density distortions on samples having loose structures. Higher cooling rates reduced deformations. Deformation-free proteins in crystalline ice were obtained by modifying the freezing conditions, and reconstructions from these samples revealed a marked improvement over vitreous ice. This procedure also increased the efficiency of cryo-EM structure determinations and was essential for high-resolution reconstructions. |
リンク | Structure / PubMed:36586403 |
手法 | EM (単粒子) |
解像度 | 1.76 - 3.2 Å |
構造データ | EMDB-28627: Cryo-EM structure of Coxsackievirus A10 (empty) embedded in crystalline ice EMDB-28638: Cryo-EM structure of Coxsackievirus A10 embedded in crystalline ice EMDB-28639, PDB-8ew0: EMDB-28640, PDB-8ew2: EMDB-32695: High resolution of apo-ferritin embedded in crystalline ice EMDB-32703: apo-ferritin embedded in crystalline ice frozen at -183 degree EMDB-32709: EV71 VLP maps embedded in crystalline ice |
由来 |
|
キーワード | VIRUS / Coxsackievirus A10 / empty Coxsackievirus A10 / crystalline ice / LYASE / glutamate dehydrogenase / GDH / Aldolase / METAL BINDING PROTEIN / ferritin / crystal ice / crystal-ice / apo-ferritin / human apo-ferritin / human ferritin / Coxsackievirus |