EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of the nucleosome transition during RNA polymerase II passage.
ジャーナル・号・ページ	Science, Vol. 362, Issue 6414, Page 595-598, Year 2018
掲載日	2018年11月2日
著者	Tomoya Kujirai / Haruhiko Ehara / Yuka Fujino / Mikako Shirouzu / Shun-Ichi Sekine / Hitoshi Kurumizaka /
PubMed 要旨	Genomic DNA forms chromatin, in which the nucleosome is the repeating unit. The mechanism by which RNA polymerase II (RNAPII) transcribes the nucleosomal DNA remains unclear. Here we report the cryo- ...Genomic DNA forms chromatin, in which the nucleosome is the repeating unit. The mechanism by which RNA polymerase II (RNAPII) transcribes the nucleosomal DNA remains unclear. Here we report the cryo-electron microscopy structures of RNAPII-nucleosome complexes in which RNAPII pauses at the superhelical locations SHL(-6), SHL(-5), SHL(-2), and SHL(-1) of the nucleosome. RNAPII pauses at the major histone-DNA contact sites, and the nucleosome interactions with the RNAPII subunits stabilize the pause. These structures reveal snapshots of nucleosomal transcription, in which RNAPII gradually tears DNA from the histone surface while preserving the histone octamer. The nucleosomes in the SHL(-1) complexes are bound to a "foreign" DNA segment, which might explain the histone transfer mechanism. These results provide the foundations for understanding chromatin transcription and epigenetic regulation.
リンク	Science / PubMed:30287617
手法	EM (単粒子) / EM (電子線結晶学)
解像度	5.6 - 9.9 Å
構造データ	6980 6a5l EMDB-6980, PDB-6a5l: RNA polymerase II elongation complex stalled at SHL(-1) of the nucleosome, with foreign DNA 手法: EM (単粒子) / 解像度: 5.6 Å 6981 6a5o EMDB-6981, PDB-6a5o: RNA polymerase II elongation complex stalled at SHL(-6) of the nucleosome 手法: EM (単粒子) / 解像度: 9.9 Å 6982 6a5p EMDB-6982, PDB-6a5p: RNA polymerase II elongation complex stalled at SHL(-5) of the nucleosome 手法: EM (単粒子) / 解像度: 7.0 Å 6983 6a5r EMDB-6983, PDB-6a5r: RNA polymerase II elongation complex stalled at SHL(-2) of the nucleosome 手法: EM (単粒子) / 解像度: 8.7 Å 6984 6a5t EMDB-6984, PDB-6a5t: RNA polymerase II elongation complex stalled at SHL(-1) of the nucleosome 手法: EM (単粒子) / 解像度: 6.7 Å 6985 6a5u EMDB-6985, PDB-6a5u: RNA polymerase II elongation complex stalled at SHL(-1) of the nucleosome, with foreign DNA, tilt conformation 手法: EM (単粒子) / 解像度: 7.6 Å 6986 6inq EMDB-6986, PDB-6inq: RNA polymerase II elongation complex stalled at SHL(-1) of the nucleosome, with foreign DNA (+1 position) 手法: EM (単粒子) / 解像度: 6.9 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	Komagataella phaffii (菌類) komagataella phaffii (strain gs115 / atcc 20864) (菌類) homo sapiens (ヒト) synthetic construct (人工物) Yeast (菌類)
キーワード	TRANSCRIPTION/RNA/DNA / nucleosome (ヌクレオソーム) / chromatin (クロマチン) / RNA polymerase (RNAポリメラーゼ) / TRANSCRIPTION (転写 (生物学)) / TRANSCRIPTION-RNA-DNA complex