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-Structure paper
タイトル | Discovery and structural characterization of a therapeutic antibody against coxsackievirus A10. |
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ジャーナル・号・ページ | Sci Adv, Vol. 4, Issue 9, Page eaat7459, Year 2018 |
掲載日 | 2018年9月19日 |
著者 | Rui Zhu / Longfa Xu / Qingbing Zheng / Yanxiang Cui / Shaowei Li / Maozhou He / Zhichao Yin / Dongxiao Liu / Shuxuan Li / Zizhen Li / Zhenqin Chen / Hai Yu / Yuqiong Que / Che Liu / Zhibo Kong / Jun Zhang / Timothy S Baker / Xiaodong Yan / Z Hong Zhou / Tong Cheng / Ningshao Xia / |
PubMed 要旨 | Coxsackievirus A10 (CVA10) recently emerged as a major pathogen of hand, foot, and mouth disease and herpangina in children worldwide, and lack of a vaccine or a cure against CVA10 infections has ...Coxsackievirus A10 (CVA10) recently emerged as a major pathogen of hand, foot, and mouth disease and herpangina in children worldwide, and lack of a vaccine or a cure against CVA10 infections has made therapeutic antibody identification a public health priority. By targeting a local isolate, CVA10-FJ-01, we obtained a potent antibody, 2G8, against all three capsid forms of CVA10. We show that 2G8 exhibited both 100% preventive and 100% therapeutic efficacy against CVA10 infection in mice. Comparisons of the near-atomic cryo-electron microscopy structures of the three forms of CVA10 capsid and their complexes with 2G8 Fab reveal that a single Fab binds a border region across the three capsid proteins (VP1 to VP3) and explain 2G8's remarkable cross-reactivities against all three capsid forms. The atomic structures of this first neutralizing antibody of CVA10 should inform strategies for designing vaccines and therapeutics against CVA10 infections. |
リンク | Sci Adv / PubMed:30255146 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.4 - 4.3 Å |
構造データ | EMDB-9603, PDB-6acz: EMDB-9604, PDB-6ad0: EMDB-9605, PDB-6ad1: EMDB-9606: |
化合物 | ChemComp-SPH: |
由来 |
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キーワード | VIRUS / CVA10 / Mature virion / Icosahedral / procapsid / A-particle / immune complex / neutralizing antibody / VIRUS/IMMUNE SYSTEM / VIRUS-IMMUNE SYSTEM complex |