EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into the regulation of human serine palmitoyltransferase complexes.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 28, Issue 3, Page 240-248, Year 2021
掲載日	2021年2月8日
著者	Yingdi Wang / Yiming Niu / Zhe Zhang / Kenneth Gable / Sita D Gupta / Niranjanakumari Somashekarappa / Gongshe Han / Hongtu Zhao / Alexander G Myasnikov / Ravi C Kalathur / Teresa M Dunn / Chia-Hsueh Lee /
PubMed 要旨	Sphingolipids are essential lipids in eukaryotic membranes. In humans, the first and rate-limiting step of sphingolipid synthesis is catalyzed by the serine palmitoyltransferase holocomplex, which ...Sphingolipids are essential lipids in eukaryotic membranes. In humans, the first and rate-limiting step of sphingolipid synthesis is catalyzed by the serine palmitoyltransferase holocomplex, which consists of catalytic components (SPTLC1 and SPTLC2) and regulatory components (ssSPTa and ORMDL3). However, the assembly, substrate processing and regulation of the complex are unclear. Here, we present 8 cryo-electron microscopy structures of the human serine palmitoyltransferase holocomplex in various functional states at resolutions of 2.6-3.4 Å. The structures reveal not only how catalytic components recognize the substrate, but also how regulatory components modulate the substrate-binding tunnel to control enzyme activity: ssSPTa engages SPTLC2 and shapes the tunnel to determine substrate specificity. ORMDL3 blocks the tunnel and competes with substrate binding through its amino terminus. These findings provide mechanistic insights into sphingolipid biogenesis governed by the serine palmitoyltransferase complex.
リンク	Nat Struct Mol Biol / PubMed:33558761 / PubMed Central
手法	EM (単粒子)
解像度	2.6 - 3.4 Å
構造データ	22598 7k0i EMDB-22598, PDB-7k0i: Human serine palmitoyltransferase complex SPTLC1/SPLTC2/ssSPTa 手法: EM (単粒子) / 解像度: 3.3 Å 22599 7k0j EMDB-22599, PDB-7k0j: Human serine palmitoyltransferase complex SPTLC1/SPLTC2/ssSPTa protomer 手法: EM (単粒子) / 解像度: 3.1 Å 22600 7k0k EMDB-22600, PDB-7k0k: Human serine palmitoyltransferase complex SPTLC1/SPLTC2/ssSPTa, 3KS-bound 手法: EM (単粒子) / 解像度: 2.6 Å 22601 7k0l EMDB-22601, PDB-7k0l: Human serine palmitoyltransferase complex SPTLC1/SPLTC2/ssSPTa, myriocin-bound 手法: EM (単粒子) / 解像度: 3.4 Å 22602 7k0m EMDB-22602, PDB-7k0m: Human serine palmitoyltransferase complex SPTLC1/SPLTC2/ssSPTa/ORMDL3, class 1 手法: EM (単粒子) / 解像度: 2.9 Å 22604 7k0n EMDB-22604, PDB-7k0n: Human serine palmitoyltransferase complex SPTLC1/SPLTC2/ssSPTa/ORMDL3, class 2 手法: EM (単粒子) / 解像度: 3.1 Å 22605 7k0o EMDB-22605, PDB-7k0o: Human serine palmitoyltransferase complex SPTLC1/SPLTC2/ssSPTa/ORMDL3, class 3 手法: EM (単粒子) / 解像度: 3.1 Å 22606 7k0p EMDB-22606, PDB-7k0p: Human serine palmitoyltransferase complex SPTLC1/SPLTC2/ssSPTa/ORMDL3, class 4 手法: EM (単粒子) / 解像度: 3.1 Å 22608 7k0q EMDB-22608, PDB-7k0q: Human serine palmitoyltransferase complex SPTLC1/SPLTC2/ssSPTa/ORMDL3, myriocin-bound 手法: EM (単粒子) / 解像度: 3.3 Å
化合物	ChemComp-PLP: PYRIDOXAL-5'-PHOSPHATE / ピリドキサ-ル5′-りん酸 / ピリドキサールリン酸 ChemComp-POV: (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / リン脂質YM / POホスファチジルコリン ChemComp-VSD: 3-Dehydrosphinganine / 3-ケトスフィンガニン ChemComp-VRP: Myriocin / ミリオシン / immunosuppressant, 抗生剤, 阻害剤YM / Myriocin
由来	homo sapiens (ヒト)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / Sphingolipid (スフィンゴ脂質)